糖尿病視網(wǎng)膜病變與外周血內(nèi)皮祖細胞關(guān)系的研究及糖尿病視網(wǎng)膜病變神經(jīng)纖維層厚度的研究
發(fā)布時間：2018-12-14 04:54
【摘要】：第一部分糖尿病視網(wǎng)膜病變與外周血內(nèi)皮祖細胞關(guān)系的研究 目的:探討不同時期糖尿病視網(wǎng)膜病變患者外周血中內(nèi)皮祖細胞(EPCs)的數(shù)量變化,分析EPCs在DR的發(fā)展過程中的作用。 方法:20例健康對照組(Control組),22例無視網(wǎng)膜病變糖尿病患者(DM組),10例非增殖性糖尿病視網(wǎng)膜病變患者(NPDR組),12例增殖性糖尿病視網(wǎng)膜病變患者(PDR組),均采空腹肘前靜脈血10ml,分別使用外周全血和單核細胞密度梯度離心法(Ficoll離心)分離單核細胞后,使用CD34+和CD309+雙熒光抗體標記,通過流式細胞儀檢測EPCs的數(shù)量,比較各組間EPCs數(shù)量的差異,實驗結(jié)果采用SPSS 17.0統(tǒng)計軟件處理。 結(jié)果:外周血中EPCs占淋巴細胞群的比例PDR組較其他三組顯著增高,P=0.000,DM組與NPDR組相比,P=0.882,差異沒有統(tǒng)計學意義。正常對照組高于DM組與NPDR組,差異有統(tǒng)計學意義。 結(jié)論:糖尿病視網(wǎng)膜病變組(DM組)和NPDR組患者外周血中EPCs的數(shù)量明顯少于正常對照組;PDR組患者外周血中EPCs數(shù)量較Control組顯著增加,EPCs可能與PDR時期眼底新生血管的發(fā)生有關(guān)。 第二部分糖尿病視網(wǎng)膜病變神經(jīng)纖維層厚度的研究 目的:研究糖尿病視網(wǎng)膜病變(Diabetic Retinopathy;DR)及全視網(wǎng)膜激光光凝(Panretinal Photocoagulation;PRP)對視乳頭旁視網(wǎng)膜神經(jīng)纖維層(Retinal Nerve Fiber Layer;RNFL)厚度及黃斑中心凹視網(wǎng)膜厚度的影響。 方法:正常對照組(Control)34例66只眼,2型糖尿病無糖尿病視網(wǎng)膜病變(Diabetes Mellitus;DM)患者組20例32只眼,非增殖性糖尿病視網(wǎng)膜病變(NPDR)患者組20例26只眼,增殖性糖尿病視網(wǎng)膜病變(PDR)患者組19例20只眼,DR行PRP治療組19例20只眼被納入研究,應(yīng)用光學相干斷層掃描儀(Optical Coherence Tomography;OCT)測量視乳頭旁神經(jīng)纖維層厚度及黃斑中心凹視網(wǎng)膜厚度,比較各組間的差異。 結(jié)果: (1)PRP治療后視乳頭旁RNFL厚度與PDR組對比明顯變薄,P=0.001;(2)黃斑區(qū)視網(wǎng)膜厚度明顯增厚主要見于增殖性糖尿病視網(wǎng)膜病變(p0.05),PRP治療后沒有明顯改變。 結(jié)論:糖尿病視網(wǎng)膜病變行PRP治療后RNFL有所變薄,黃斑區(qū)視網(wǎng)膜厚度無明顯改變。
[Abstract]:Part one the relationship between diabetic retinopathy and peripheral blood endothelial progenitor cells objective: to investigate the changes of (EPCs) in peripheral blood of patients with diabetic retinopathy at different stages. The role of EPCs in the development of DR is analyzed. Methods: twenty healthy controls (Control group), 22 patients without retinopathy diabetes mellitus (DM group), 10 patients with non-proliferative diabetic retinopathy (NPDR group), 12 patients with proliferative diabetic retinopathy (PDR group), 10 patients with non-proliferative diabetic retinopathy (NPDR group), 12 patients with proliferative diabetic retinopathy (PDR group) were enrolled in this study. 10 ml of fasting anterior cubital venous blood was collected. Monocytes were separated by peripheral blood and monocyte density gradient centrifugation (Ficoll) respectively. CD34 and CD309 double fluorescent antibodies were used to detect the number of EPCs by flow cytometry. The results of the experiment were processed by SPSS 17.0 software. Results: the ratio of EPCs in peripheral blood to lymphocyte group in PDR group was significantly higher than that in the other three groups. There was no significant difference between PDR group and NPDR group. Normal control group was higher than DM group and NPDR group, the difference was statistically significant. Conclusion: the number of EPCs in peripheral blood of diabetic retinopathy group (DM group) and NPDR group is significantly lower than that of normal control group, and that of PDR group is significantly higher than that of Control group. EPCs may be related to the occurrence of fundus neovascularization in PDR. The second part of the study on the thickness of nerve fiber layer in diabetic retinopathy objective: to study diabetic retinopathy (Diabetic Retinopathy;DR) and total retinal laser photocoagulation (Panretinal Photocoagulation;) The effect of PRP on the retinal nerve fiber layer (Retinal Nerve Fiber Layer;RNFL) thickness and macular fovea retinal thickness. Methods: in normal control group, there were 34 cases (66 eyes) of (Control), type 2 diabetes without diabetic retinopathy (Diabetes Mellitus;). DM (n = 20, n = 32), (NPDR) (n = 20, n = 26), (PDR) (n = 19, n = 20) and PRP (n = 19, n = 20). Applied optical coherence tomography (Optical Coherence Tomography; The thickness of parapapillary nerve fiber layer and macular fovea retina were measured by OCT. Results: (1) the thickness of parapapillary RNFL in PRP group was significantly thinner than that in PDR group. (2) Retinal thickening of macular area was mainly seen in proliferative diabetic retinopathy (p0. 05), PRP). Conclusion: RNFL became thinner and retinal thickness of macular area did not change after PRP treatment for diabetic retinopathy.
【學位授予單位】：南京醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R774.1
本文編號：2377961
[Abstract]:Part one the relationship between diabetic retinopathy and peripheral blood endothelial progenitor cells objective: to investigate the changes of (EPCs) in peripheral blood of patients with diabetic retinopathy at different stages. The role of EPCs in the development of DR is analyzed. Methods: twenty healthy controls (Control group), 22 patients without retinopathy diabetes mellitus (DM group), 10 patients with non-proliferative diabetic retinopathy (NPDR group), 12 patients with proliferative diabetic retinopathy (PDR group), 10 patients with non-proliferative diabetic retinopathy (NPDR group), 12 patients with proliferative diabetic retinopathy (PDR group) were enrolled in this study. 10 ml of fasting anterior cubital venous blood was collected. Monocytes were separated by peripheral blood and monocyte density gradient centrifugation (Ficoll) respectively. CD34 and CD309 double fluorescent antibodies were used to detect the number of EPCs by flow cytometry. The results of the experiment were processed by SPSS 17.0 software. Results: the ratio of EPCs in peripheral blood to lymphocyte group in PDR group was significantly higher than that in the other three groups. There was no significant difference between PDR group and NPDR group. Normal control group was higher than DM group and NPDR group, the difference was statistically significant. Conclusion: the number of EPCs in peripheral blood of diabetic retinopathy group (DM group) and NPDR group is significantly lower than that of normal control group, and that of PDR group is significantly higher than that of Control group. EPCs may be related to the occurrence of fundus neovascularization in PDR. The second part of the study on the thickness of nerve fiber layer in diabetic retinopathy objective: to study diabetic retinopathy (Diabetic Retinopathy;DR) and total retinal laser photocoagulation (Panretinal Photocoagulation;) The effect of PRP on the retinal nerve fiber layer (Retinal Nerve Fiber Layer;RNFL) thickness and macular fovea retinal thickness. Methods: in normal control group, there were 34 cases (66 eyes) of (Control), type 2 diabetes without diabetic retinopathy (Diabetes Mellitus;). DM (n = 20, n = 32), (NPDR) (n = 20, n = 26), (PDR) (n = 19, n = 20) and PRP (n = 19, n = 20). Applied optical coherence tomography (Optical Coherence Tomography; The thickness of parapapillary nerve fiber layer and macular fovea retina were measured by OCT. Results: (1) the thickness of parapapillary RNFL in PRP group was significantly thinner than that in PDR group. (2) Retinal thickening of macular area was mainly seen in proliferative diabetic retinopathy (p0. 05), PRP). Conclusion: RNFL became thinner and retinal thickness of macular area did not change after PRP treatment for diabetic retinopathy.
【學位授予單位】：南京醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R774.1
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