【摘要】：目的研究髓源性抑制細(xì)胞(myeloid derived suppressor cells,MDSC)與鼻咽癌細(xì)胞上皮-間質(zhì)轉(zhuǎn)化(epithelialmesenchymal transition,EMT)的關(guān)系及對(duì)鼻咽癌細(xì)胞侵襲轉(zhuǎn)移的影響。方法用流式細(xì)胞技術(shù)檢測(cè)16例鼻咽癌患者以及9例健康對(duì)照者骨髓中MDSC所占比例,并分離鼻咽癌組織中MDSC;將MDSC與鼻咽癌細(xì)胞株CNE-2共培養(yǎng),觀察CNE-2細(xì)胞形態(tài)學(xué)的改變;應(yīng)用q RT-PCR技術(shù)從RNA水平檢測(cè)共培養(yǎng)前后CNE-2細(xì)胞上皮-間質(zhì)轉(zhuǎn)化相關(guān)基因(E-cadherin,N-cadherin,Vimentin,Snail)的改變;進(jìn)一步采用Western blot技術(shù)從蛋白水平檢測(cè)其表達(dá)變化;繼而通過transwell小室及劃痕實(shí)驗(yàn)檢測(cè)MDSC對(duì)CNE-2細(xì)胞侵襲轉(zhuǎn)移能力的影響。結(jié)果鼻咽癌患者骨髓中MDSC所占比例(10.37%)明顯高于健康對(duì)照者(1.65%);MDSC與CNE-2細(xì)胞共培養(yǎng)后,CNE-2細(xì)胞在形態(tài)學(xué)上發(fā)生EMT;q RT-PCR和Western blot分別從RNA和蛋白水平證實(shí)CNE-2細(xì)胞上皮表型的相關(guān)基因(E-cadherin)表達(dá)下調(diào),間質(zhì)表型的相關(guān)基因(N-cadherin,Vimentin,Snail)表達(dá)上調(diào);transwell小室侵襲實(shí)驗(yàn)證實(shí)共培養(yǎng)后CNE-2細(xì)胞的侵襲能力增強(qiáng);劃痕實(shí)驗(yàn)證實(shí)共培養(yǎng)后CNE-2細(xì)胞的遷移能力增強(qiáng)。結(jié)論 MDSC通過誘導(dǎo)EMT促進(jìn)鼻咽癌細(xì)胞的侵襲轉(zhuǎn)移能力。
[Abstract]:Objective to study the relationship between myeloid derived suppressor cells (MDSC) and epithelialmesenchymal transition (EMT) in nasopharyngeal carcinoma cells and the influence on the invasion and metastasis of nasopharyngeal carcinoma cells. Methods flow cytometry was used to detect the proportion of MDSC in 16 cases of nasopharyngeal carcinoma and 9 healthy controls. MDSC was isolated and isolated from nasopharyngeal carcinoma tissue. MDSC was co cultured with nasopharyngeal carcinoma cell line CNE-2 to observe the morphological changes of CNE-2 cells. The changes of CNE-2 cell epithelial mesenchymal transformation related genes (E-cadherin, N-cadherin, Vimentin, Snail) before and after co culture were detected by Q RT-PCR technology. The effect of MDSC on the invasion and metastasis of CNE-2 cells was detected by the Transwell chamber and scratch test. Results the proportion of MDSC in the bone marrow of the patients with nasopharyngeal carcinoma (10.37%) was significantly higher than that of the healthy controls (1.65%). After co culture of MDSC and CNE-2 cells, the CNE-2 cells were morphologically EMT; Q RT-PCR and Western. Blot confirmed that the expression of CNE-2 cell epithelial phenotype related genes (E-cadherin) was down regulated from RNA and protein levels, and the expression of interstitial phenotype related genes (N-cadherin, Vimentin, Snail) was up-regulated, and Transwell chamber invasion experiment confirmed that the invasion ability of CNE-2 cells increased after co culture, and the scratch test confirmed the migration energy of CNE-2 cells after co culture. Conclusion MDSC can promote the invasion and metastasis of nasopharyngeal carcinoma cells by inducing EMT.
【作者單位】： 解放軍第324
【分類號(hào)】：R739.63

【參考文獻(xiàn)】

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1 馬吉春;林江;姚冬明;鄧兆群;錢煒;錢軍;陳芹;楊靜;李云;陳星星;聞向梅;郭z，

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