COX-2、iNOS在原發(fā)性翼狀胬肉中的表達及其相關研究
發(fā)布時間：2018-07-20 17:56
【摘要】：目的：①比較不同時期原發(fā)性翼狀胬肉上皮細胞中環(huán)氧合酶-2(cyclooxygenase-2、COX-2)、誘導型一氧化氮合酶(induce-nitricoxide synthase、iNOS)的表達；②探討環(huán)氧合酶2 (COX-2)和誘導型一氧化氮合酶(iNOS)對原發(fā)性翼狀胬肉發(fā)生、發(fā)展的影響及其作用機制；探討細胞凋亡在原發(fā)性翼狀胬肉組織中的表達及其在發(fā)生發(fā)展過程中的作用。 方法：選取2008年至2009年石河子大學第一附屬醫(yī)院眼科行手術切除的56例原發(fā)性翼狀胬肉患者(56只眼)。按病變進行情況分為進行期和靜止期,進行期40例(40只眼),靜止期16例(16只眼)各組兼顧年齡、地區(qū)、種族及性別差異,所有患者排除眼部及身體其他部位疾病,排除其它眼科及全身疾病,其中男性19例,女性37例；年齡29～85歲,平均59歲；病程2-30年。正常結膜組織取自同時期行其他眼科手術并排除結膜疾病的20例患者術中所切除的少許正常結膜組織(均為手術中廢棄不要的組織),男性12例,女性8例；年齡40-60歲,平均56歲。兩組年齡比較,無顯著性差異。取材前均經(jīng)患者知情同意。全部標本均經(jīng)石河子大學第一附屬醫(yī)院病理科統(tǒng)一切片確診。 病理標本做連續(xù)切片,厚3μm,分別進行HE染色和免疫組織化學Elivision染色。免疫組化結果判定綜合染色強度及陽性細胞數(shù)兩方面進行分析。同時采用脫氧核苷酸末端轉移酶介導的脫氧尿苷三磷酸末端標記法(terminal deoxynucleotidyl transferase mediated 2'-deoxyuridine 5'-triphosphate-biotin nick end labeling, TUNEL),檢測不同時期原發(fā)性翼狀胬肉細胞中凋亡細胞的表達。 結果：①COX-2在原發(fā)性翼狀胬肉組織中表達上調(diào),靜止期、進行期與正常結膜之間比較差異有顯著性意義,P0.001；靜止期與正常結膜,進行期與正常結膜,以及靜止期與進行期之間比較差異有意義,P0.05。iNOS在原發(fā)性翼狀胬肉組織中表達上調(diào),靜止期、進行期與正常結膜之間比較差異有顯著性意義,P0.001；進行期與正常結膜,以及靜止期與進行期之間比較差異均有顯著意義；靜止期與正常結膜之間比較差異無意義,P0.05。②不同時期的原發(fā)性翼狀胬肉組織中均發(fā)現(xiàn)凋亡細胞的表達。 結論：一、COX-2和iNOS在原發(fā)性翼狀胬肉發(fā)生發(fā)展中起一定的促進作用。二、COX-2和iNOS對原發(fā)性翼狀胬肉發(fā)生發(fā)展起協(xié)同作用。三、原發(fā)性翼狀胬肉中COX-2和iNOS引起的細胞凋亡與病程發(fā)展有一定關系。
[Abstract]:Objective to compare the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (induce-nitricoxide) in primary pterygium cells at different stages of primary pterygium. To investigate the role of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in the pathogenesis of primary pterygium. To investigate the expression of apoptosis in primary pterygium and its role in the development of pterygium. Methods: 56 patients (56 eyes) with primary pterygium were selected from 2008 to 2009 in the first affiliated Hospital of Shihezi University. There were 40 cases (40 eyes) in the progressive stage and 16 eyes (16 eyes) in the stationary stage according to the condition of the disease. All the patients were excluded from the eye and other parts of the body by taking into account the differences of age, region, race and sex. Other ophthalmic and systemic diseases were excluded, including 19 males and 37 females; age 2985 years, mean 59 years; course of disease 2-30 years. Normal conjunctival tissue was obtained from 20 patients who underwent other ophthalmic operations at the same time and excluded conjunctival diseases. They were 12 males and 8 females aged 40-60 years. The average age is 56. There was no significant difference in age between the two groups. Informed consent of the patient was obtained before the material was taken. All specimens were confirmed by unified section of Department of Pathology, first affiliated Hospital of Shihezi University. The pathological specimens were stained by HE staining and immunohistochemical Elivision staining respectively with 3 渭 m thick sections. The immunohistochemical results were used to determine the comprehensive staining intensity and the number of positive cells. The expression of apoptotic cells in primary pterygium cells at different stages was detected by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate end labeling (terminal deoxynucleotidyl transferase mediated 2'-deoxyuridine 5'-triphosphate-biotin nick end labeling, Tunel). Results the expression of COX-2 in primary pterygium was significantly higher than that in normal conjunctiva (P 0.001), the expression of COX-2 in primary pterygium was significantly higher than that in normal conjunctiva (P 0.001), the expression of COX-2 in primary pterygium was significantly higher than that in normal conjunctiva (P 0.001). The expression of P0.05.iNOS was up-regulated in primary pterygium tissue, and there was significant difference between resting stage, progressive stage and normal conjunctiva (P0.001). The expression of apoptotic cells was found in pterygium tissues at different stages of pterygium with no significant difference between resting phase and normal conjunctiva. Conclusion: COX-2 and iNOS play an important role in the development of primary pterygium. COX-2 and iNOS play a synergistic role in the development of primary pterygium. Thirdly, the apoptosis induced by COX-2 and iNOS in primary pterygium is related to the progression of pterygium.
【學位授予單位】：石河子大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R777.33
本文編號：2134302
[Abstract]:Objective to compare the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (induce-nitricoxide) in primary pterygium cells at different stages of primary pterygium. To investigate the role of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in the pathogenesis of primary pterygium. To investigate the expression of apoptosis in primary pterygium and its role in the development of pterygium. Methods: 56 patients (56 eyes) with primary pterygium were selected from 2008 to 2009 in the first affiliated Hospital of Shihezi University. There were 40 cases (40 eyes) in the progressive stage and 16 eyes (16 eyes) in the stationary stage according to the condition of the disease. All the patients were excluded from the eye and other parts of the body by taking into account the differences of age, region, race and sex. Other ophthalmic and systemic diseases were excluded, including 19 males and 37 females; age 2985 years, mean 59 years; course of disease 2-30 years. Normal conjunctival tissue was obtained from 20 patients who underwent other ophthalmic operations at the same time and excluded conjunctival diseases. They were 12 males and 8 females aged 40-60 years. The average age is 56. There was no significant difference in age between the two groups. Informed consent of the patient was obtained before the material was taken. All specimens were confirmed by unified section of Department of Pathology, first affiliated Hospital of Shihezi University. The pathological specimens were stained by HE staining and immunohistochemical Elivision staining respectively with 3 渭 m thick sections. The immunohistochemical results were used to determine the comprehensive staining intensity and the number of positive cells. The expression of apoptotic cells in primary pterygium cells at different stages was detected by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate end labeling (terminal deoxynucleotidyl transferase mediated 2'-deoxyuridine 5'-triphosphate-biotin nick end labeling, Tunel). Results the expression of COX-2 in primary pterygium was significantly higher than that in normal conjunctiva (P 0.001), the expression of COX-2 in primary pterygium was significantly higher than that in normal conjunctiva (P 0.001), the expression of COX-2 in primary pterygium was significantly higher than that in normal conjunctiva (P 0.001). The expression of P0.05.iNOS was up-regulated in primary pterygium tissue, and there was significant difference between resting stage, progressive stage and normal conjunctiva (P0.001). The expression of apoptotic cells was found in pterygium tissues at different stages of pterygium with no significant difference between resting phase and normal conjunctiva. Conclusion: COX-2 and iNOS play an important role in the development of primary pterygium. COX-2 and iNOS play a synergistic role in the development of primary pterygium. Thirdly, the apoptosis induced by COX-2 and iNOS in primary pterygium is related to the progression of pterygium.
【學位授予單位】：石河子大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R777.33
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