發(fā)布時間：2018-06-27 10:35

  本文選題：慢性鼻-鼻竇炎 + 天然免疫�。� 參考：《臨床耳鼻咽喉頭頸外科雜志》2014年17期

【摘要】：目的:通過檢測天然免疫因子Toll樣受體2(TLR2)和Toll樣受體4(TLR4)在慢性鼻-鼻竇炎不同臨床分型以及對照組中的表達情況,探討TLR在慢性鼻-鼻竇炎發(fā)病機制中的作用。方法:采用免疫組織化學(xué)方法分別檢測TLR2和TLR4在慢性鼻-鼻竇炎伴鼻息肉組(A組,21例),慢性鼻-鼻竇炎無鼻息肉組(B組,15例),復(fù)發(fā)性慢性鼻-鼻竇炎伴鼻息肉組(C組,11例)及對照組(D組,13例)中的表達情況。顯微鏡下計數(shù)陽性細(xì)胞表達情況,等級資料采用Mann-Whitney U進行統(tǒng)計分析,組間用單因素的方差進行統(tǒng)計分析。結(jié)果:1TLR2和TLR4有相同的表達特點,主要表達部位集中在上皮基膜全層、腺體細(xì)胞的細(xì)胞質(zhì)及極少數(shù)炎性細(xì)胞,如單核細(xì)胞和漿細(xì)胞的細(xì)胞質(zhì),偶有不明細(xì)胞的細(xì)胞核陽性表達;2A、B、C、D組鼻腔黏膜組織中TLR2的表達分別為10.18±16.80、27.00±12.07、7.55±14.69、9.40±17.83,A、C、D組與B組比較,均差異有統(tǒng)計學(xué)意義(均P0.05);3A、B、C、D組鼻腔黏膜組織中TLR4的表達分別為11.30±19.22、36.02±17.75、8.51±9.22、7.75±4.49,A、C、D組與B組比較,均差異有統(tǒng)計學(xué)意義(均P0.05)。結(jié)論:鼻腔黏膜上皮能產(chǎn)生天然免疫因子TLR2和TLR4;TLR2和TLR4在慢性鼻-鼻竇炎各臨床表型中的差異性表達提示其在慢性鼻-鼻竇炎的發(fā)病中發(fā)揮了一定作用。
[Abstract]:Objective: to investigate the role of TLR in the pathogenesis of chronic rhinosinusitis by detecting the expression of Toll-like receptor 2 (TLR2) and Toll-like receptor 4 (TLR4) in different clinical types of chronic rhinosinusitis and control group. Methods: immunohistochemical method was used to detect TLR2 and TLR4 in 21 patients with chronic rhinosinusitis with nasal polyps (group A), 15 patients with chronic rhinosinusitis without nasal polyps (group B) and 15 patients with recurrent chronic rhinosinusitis with nasal polyps. In group C (n = 11) and control group (group D, n = 13). The expression of positive cells was counted under microscope. Mann-Whitney U was used to analyze the grade data and the variance of single factor was used for statistical analysis among groups. Results the expression of TLR2 and TLR4 was the same, mainly in the whole layer of epithelial basement membrane, the cytoplasm of glandular cells and the cytoplasm of a few inflammatory cells, such as monocytes and plasma cells. The positive expression of TLR2 in nasal mucosa of group D was 10.18 鹵16.80 鹵27.00 鹵12.07 鹵7.55 鹵14.69 鹵9.40 鹵17.83C (P 0.05). The expression of TLR4 in nasal mucosa of group D was 11.30 鹵19.225.02 鹵17.77.51 鹵9.227.75 鹵4.49.75 鹵4.49.9. the expression of TLR4 in nasal mucosa of group D was significantly higher than that of group B (P 0.05). The difference was statistically significant (P0.05). Conclusion: the differential expression of TLR2 and TLR4TLR2 and TLR4 in the clinical phenotypes of chronic rhinosinusitis suggests that TLR2 and TLR4 may play a role in the pathogenesis of chronic rhinosinusitis.
【作者單位】： 哈爾濱醫(yī)科大學(xué)附屬第二醫(yī)院耳鼻咽喉頭頸外科;海林市人民醫(yī)院耳鼻咽喉科;
【基金】：黑龍江省教育廳科技面上項目(No:12541272)
【分類號】：R765.41

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