本文選題：去整合素 + 基質金屬蛋白酶��； 參考：《廣西醫(yī)科大學》2011年碩士論文

【摘要】：目的:研究去整合素Kistrin作用下,晶狀體摘取術后兔晶狀體上皮細胞( Lens epithelium cells , LECs )中基質金屬蛋白酶2 ( Matrix metalloproteinases 2,MMP-2)mRNA和其抑制因子組織性金屬蛋白酶抑制物2(Tissue inhibitor of metalloproteinase 2,TIMP-2)mRNA表達的變化,探討kistrin作用的分子機制。 方法:將12只新西蘭大白兔隨機分為實驗組和對照組,每組6只,均行右眼ECLE建立后V⒛せ熳莵\汀J醣鮮笛樽檳掖閬謐⑷肱ǘ任?0μg·L ~(-1)的Kistrin 0.2 mL,對照組注入等量生理鹽水,隨機選6只左眼為空白組。術后1 d、2d、3 d、7 d、14 d在裂隙燈下觀察實驗動物眼部情況。觀察14天后取后V⒛ば惺凳庇舛縋CR檢測MMP-2mRNA和TIMP-2mRNA的表達量。 結果:正常的兔后V⒛ど希ǹ瞻鬃椋㎝MP-2mRNA和TIMP-2mRNA表達量少,分別為0.127±0.039,2.485±0.507。白內障術后14天對照組兩個基因表達量分別為3.785±0.934,19.903±10.499,均較空白組明顯升高(p0.05)。實驗組MMP-2mRNA的表達量為0.401±0.253,較對照組明顯降低(p0.05),而與空白組比較無明顯差異(p0.05)。實驗組TIMP-2mRNA的表達量為16.119±3.773,與對照組比較無明顯差異(p0.05),較空白組明顯升高(p0.05)。 結論:晶狀體囊外摘除術后使用Kistrin,MMP-2mRNA表達降低,TIMP-2mRNA的表達沒有明顯改變,可能是晶狀體上皮細胞增殖受抑制的關鍵因素。
[Abstract]:Objective: to study the expression of matrix metalloproteinase 2 (Matrix metalloproteinases 2 mali-2md2 and tissue inhibitor of metalloproteinase (2(Tissue inhibitor of metalloproteinase 2) TIMP 2 in rabbit lens epithelial cells (LECs) after lens extraction (LECs) with disintegrin Kistrin, and to investigate the expression of MMP-2 mRNA and tissue inhibitor of metalloproteinase (2(Tissue inhibitor of metalloproteinase 2) in rabbit lens epithelial cells after lens extraction. To study the molecular mechanism of kistrin action. Methods: twelve New Zealand white rabbits were randomly divided into experimental group (n = 6) and control group (n = 6). After the establishment of right eye ECLE, Kistrin 0.2 mL was injected into the control group. Six left eyes were randomly selected as blank group. The eyes of experimental animals were observed under slit lamp at 1 day, 2 days, 3 days, 7 days and 14 days after operation. After 14 days of observation, V20 was removed. Ill-fated? The expression of MMP-2mRNA and TIMP-2mRNA was detected by lower CR. Results: normal rabbits after V20? The expression of MP-2mRNA and TIMP-2mRNA were 0.127 鹵0.039 鹵0.507 and 2.485 鹵0.507 respectively. On the 14th day after cataract operation, the expression of two genes in the control group was 3.785 鹵0.934 鹵19.903 鹵10.499, which was significantly higher than that in the blank group. The expression of MMP-2mRNA in the experimental group was 0.401 鹵0.253, which was significantly lower than that in the control group, but there was no significant difference between the control group and the control group. The expression of TIMP-2mRNA in the experimental group was 16.119 鹵3.773. There was no significant difference between the experimental group and the control group (P 0.05), but it was significantly higher than that in the blank group. Conclusion: after extracapsular lens excision, the expression of Kistrin MMP 2 mRNA decreased and TIMP-2 mRNA expression did not change significantly, which may be the key factor to inhibit the proliferation of lens epithelial cells.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R779.6
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本文編號：1851963