本文選題：豚鼠　切入點(diǎn)：動(dòng)物模型　出處：《復(fù)旦大學(xué)》2011年碩士論文　論文類型：學(xué)位論文

【摘要】：第一部分豚鼠內(nèi)淋巴積水模型的建立 目的：探討豚鼠內(nèi)淋巴積水模型的制作方法,為后續(xù)實(shí)驗(yàn)奠定基礎(chǔ)。方法：健康豚鼠30只,隨機(jī)分為對照組、實(shí)驗(yàn)組1、實(shí)驗(yàn)組2,各10只,實(shí)驗(yàn)組1、實(shí)驗(yàn)組2行右側(cè)內(nèi)淋巴囊切除及部分內(nèi)淋巴囊阻塞以建立內(nèi)淋巴積水動(dòng)物模型,對照組右側(cè)施行假手術(shù),于術(shù)后四周、術(shù)后八周分別處死實(shí)驗(yàn)組1、實(shí)驗(yàn)組2,術(shù)后八周處死對照組,取雙側(cè)聽泡進(jìn)行切片并染色。結(jié)果：對照組及左側(cè)耳蝸均未出現(xiàn)膜迷路積水,實(shí)驗(yàn)組1、實(shí)驗(yàn)組2出現(xiàn)程度不同膜迷路積水,前庭膜向前庭階膨出。實(shí)驗(yàn)組2較實(shí)驗(yàn)組1顯示前庭膜膨出更嚴(yán)重。結(jié)論：內(nèi)淋巴囊切除及部分內(nèi)淋巴囊阻塞術(shù)可以導(dǎo)致豚鼠內(nèi)淋巴積水,有效建立了膜迷路積水的動(dòng)物模型。 第二部分豚鼠眼震電圖的記錄 目的：探討豚鼠的眼震電圖的記錄方法,從而了解內(nèi)淋巴積水對豚鼠外周前庭頻率感受功能的損傷特點(diǎn)。方法：健康豚鼠30只,隨機(jī)分為正常對照組、前庭破壞組、實(shí)驗(yàn)組,各10只,實(shí)驗(yàn)組行右側(cè)內(nèi)淋巴囊切除及部分內(nèi)淋巴囊阻塞以建立內(nèi)淋巴積水動(dòng)物模型,前庭破壞組行前庭破壞術(shù)。用冰水試驗(yàn)和旋轉(zhuǎn)試驗(yàn)誘發(fā)并記錄正常對照組、前庭破壞組的眼震電圖,于術(shù)后四周用冰水試驗(yàn)和旋轉(zhuǎn)試驗(yàn)誘發(fā)并記錄實(shí)驗(yàn)組的眼震電圖。眼震電圖記錄眼震持續(xù)時(shí)間和頻率。結(jié)果：冰水試驗(yàn)正常對照組可引出明顯眼震,并持續(xù)較長時(shí)間。實(shí)驗(yàn)組術(shù)后四周冰水刺激未引出眼震,前庭破壞組冰水試驗(yàn)無眼震。在旋轉(zhuǎn)試驗(yàn)中,前庭破壞組未引出明顯眼震。正常對照組和實(shí)驗(yàn)組術(shù)后四周時(shí)左、右眼震慢相速度無統(tǒng)計(jì)學(xué)差異(P0.05)。在甩頭試驗(yàn)中,前庭破壞組無明顯眼動(dòng),正常對照組和實(shí)驗(yàn)組術(shù)后四周時(shí)左、右眼動(dòng)速度無統(tǒng)計(jì)學(xué)差異(P0.05)。結(jié)論：內(nèi)淋巴積水主要影響前庭器官的低頻旋轉(zhuǎn)感受功能,高頻、高速旋轉(zhuǎn)無明顯影響。 第三部分內(nèi)淋巴積水豚鼠各時(shí)期眼震變化的記錄 目的：探討內(nèi)淋巴豚鼠不同時(shí)期眼震的變化情況,從而了解內(nèi)淋巴積水不同時(shí)期對前庭功能的損傷特點(diǎn)。方法：健康豚鼠40只,隨機(jī)分為對照組、術(shù)后兩周組、術(shù)后四周組、術(shù)后八周組4組,術(shù)后組均行右側(cè)內(nèi)淋巴囊切除及部分內(nèi)淋巴囊阻塞以建立內(nèi)淋巴積水動(dòng)物模型,于相應(yīng)時(shí)間段用冰水試驗(yàn)和旋轉(zhuǎn)試驗(yàn)誘發(fā)并記錄眼震電圖。并同期處死豚鼠,取水平半規(guī)管、上半規(guī)管、后半規(guī)管、橢圓 囊、球囊標(biāo)本,進(jìn)行掃描、透射電鏡觀察。結(jié)果：冰水試驗(yàn)時(shí)術(shù)后兩周組有4只豚鼠(40%)引出眼震,但是眼震較微弱,持續(xù)時(shí)間較對照組短、眼震速度較慢。其余術(shù)后各組均未引出明顯眼震。旋轉(zhuǎn)試驗(yàn)時(shí)對照組、術(shù)后兩周組、術(shù)后四周組、術(shù)后八周組左、右眼震慢相速度無明顯差異(P0.05)。甩頭試驗(yàn)時(shí),對照組、術(shù)后兩周組、術(shù)后四周組、術(shù)后八周組左、右眼動(dòng)速度無明顯差異(P0.05)。電鏡中可見術(shù)后兩周時(shí)毛細(xì)胞無明顯改變,四周時(shí)部分區(qū)域毛細(xì)胞排列不規(guī)整,纖毛變短、倒伏,Ⅱ型毛細(xì)胞腫脹、空泡,Ⅰ型毛細(xì)胞形態(tài)較完整。八周時(shí)部分區(qū)域出現(xiàn)毛細(xì)胞的缺失,Ⅱ型毛細(xì)胞出現(xiàn)變形、皺縮,Ⅰ型毛細(xì)胞形態(tài)無明顯變形。結(jié)論：內(nèi)淋巴積水主要影響前庭器官的低頻旋轉(zhuǎn)感受功能,對前庭毛細(xì)胞的損傷多以Ⅱ型毛細(xì)胞為主。Ⅱ型毛細(xì)胞的損傷和缺失可能是低頻旋轉(zhuǎn)感受功能損害和缺失的主要細(xì)胞學(xué)基礎(chǔ)。
[Abstract]:The first part of the model of endolymphatic hydrops in guinea pigs
Objective: To explore the method of making guinea pig endolymphatic hydrops model, lay the foundation for the subsequent experiments. Methods: 30 guinea pigs were randomly divided into control group, experimental group 1, experimental group 2, 10 rats in each experimental group 1, experimental group 2 underwent right hepatectomy and partial endolymphatic sac endolymphatic sac occlusion to establish endolymphatic hydrops animal model, the control group underwent sham operation on the right side, four weeks after surgery, eight weeks after operation respectively. The experimental group 1, experimental group 2, control group were sacrificed eight weeks after operation, the bilateral auditory vesicle were sliced and stained. Results: the control group and the left cochlea were not hydrolabyrinth, experiment group 1, experimental group 2 different degrees of hydrolabyrinth, vestibular membrane forward bulging. The court order 2 in experimental group than in the experimental group 1 showed more severe vestibular meningocele. Conclusion: endolymphatic sac excision and endolymphatic sac occlusion can lead to guinea pig endolymphatic hydrops, established a membranous labyrinth An animal model of water accumulation.
The record of second parts of guinea pig nystagmus
Objective: To explore the method of recording electronystagmogram of guinea pigs, so as to understand the injury characteristics of endolymphatic hydrops in guinea pig vestibular function of frequency. Methods: 30 guinea pigs were randomly divided into normal control group, vestibular damage group, experimental group, 10 rats in each experimental group underwent right hepatectomy and partial in endolymphatic sac in order to establish the obstruction of endolymphatic sac endolymphatic hydrops animal model of vestibular damage group underwent vestibular destruction. With ice water test and rotation test induced and recorded in normal control group, damage group vestibular nystagmus, at four weeks after surgery with ice water test and rotation test induced and electronystagmography recording experimental group electronystagmogram duration were recorded. And the frequency of ice water test. Results: the normal control group can lead to obvious nystagmus, and lasting a long time. After the experimental group around the ice water stimulation did not elicit vestibular nystagmus, nystagmus. No damage group ice test In the rotarod test, vestibular damage were not elicited obvious nystagmus. Normal control group and experimental group four weeks after surgery when left, there was no significant difference in right nystagmus slow phase velocity (P0.05). In the head test, no obvious damage to vestibular eye group, normal control group and experimental group after four weeks left, no significant difference right eye velocity (P0.05). Conclusion: endolymphatic hydrops mainly affects the vestibular organ low-frequency rotation sensation function, high frequency, high rotating speed has no obvious effect.
Changes of nystagmus in third parts of endolymphatic hydrops in guinea pigs
Objective: To investigate the changes of guinea pig endolymphatic nystagmus, so as to understand the endolymphatic hydrops damage characteristics of vestibular function in different period. Methods: 40 guinea pigs were randomly divided into control group, two weeks after surgery group, postoperative 4 weeks group, eight weeks after the operation of 4 groups, postoperative groups the right part of the endolymphatic sac excision and endolymphatic sac occlusion to establish endolymphatic hydrops animal model, in the corresponding time period with ice water test and rotation test induced and recording electronystagmogram and over the same period. The guinea pigs were sacrificed, the horizontal semicircular canal, anterior semicircular canal, posterior semicircular canal, oval
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本文編號：1606464