高鋁暴露地區(qū)再生障礙性貧血患者免疫細胞功能的變化
發(fā)布時間：2019-03-08 15:59
【摘要】：目的:研究高鋁暴露地區(qū)再生障礙性貧血(aplastic anemia,AA)患者免疫細胞功能的變化及其臨床特點,分析不同血鋁水平與免疫細胞和免疫因子的關系。方法:選取確診AA病例62例,根據(jù)飲用水鋁含量及病例來源分為2組,飲用水鋁含量300μg/L并長期居住于鋁工業(yè)地區(qū)為高鋁暴露地區(qū)AA組,共33例;飲用水鋁含量300ug/L并長期居住于非鋁工業(yè)地區(qū)為非高鋁暴露地區(qū)AA組,共29例,同時選取高鋁暴露地區(qū)及非高鋁暴露地區(qū)健康人群各30例作為對照組。采用電感藕合等離子體質(zhì)譜(inductivity coupled plasma mass spectrometry,ICP-MS)方法檢測血清鋁水平,酶聯(lián)免疫吸附法(enzyme linked immuno sorbent assay,ELISA)法檢測細胞因子IL-10、IL-12、IL-17及INF-γ表達情況,流式細胞術(flow cytometry,FCM)檢測淋巴細胞亞群比例,同時收集患者免疫球蛋白、補體C3、C4表達水平數(shù)據(jù)及臨床資料。分析高鋁暴露地區(qū)AA患者免疫細胞及免疫因子變化特點,探討血清鋁與免疫指標的關系。結(jié)果:1、高鋁地區(qū)人群(高鋁AA及高鋁健康組)血清鋁水平高于非高鋁地區(qū)人群(非高鋁AA及非高鋁健康)(P0.05)。高鋁暴露地區(qū)AA患者血清鋁水平增高,顯著高于非高鋁暴露地區(qū)AA組及健康對照組(均P0.01)。2、高鋁暴露地區(qū)AA患者的貧血程度、骨髓造血組織減少程度明顯重于非高鋁暴露地區(qū)AA組(均P0.05),但兩組患者的發(fā)病年齡、性別、白細胞、中性粒細胞數(shù)目、網(wǎng)織紅細胞數(shù)量、血小板數(shù)量、骨髓增生程度無明顯差異。3、高鋁暴露地區(qū)AA患者CD4+T淋巴細胞比例、CD4+/CD8+高于非高鋁暴露地區(qū)AA組及健康對照組(均P0.05),CD8+T淋巴細胞比例低于非高鋁地區(qū)AA組及健康對照組(均P0.05),CD3+、CD+19、NK細胞比例兩組比較無明顯統(tǒng)計學差異(均P0.05)。CD4+T、CD8+T淋巴細胞比例、CD4+/CD8+與血清鋁水平未見相關性(r=0.169,P=0.067;r=0.072,P=0.438;r=-0.039,P=0.679)。4、高鋁暴露地區(qū)AA患者IL-10、IFN-γ水平高于非高鋁暴露地區(qū)AA組(均P0.05)。IL-17、IL-12水平兩組比較無統(tǒng)計學差異(均P0.05)。血清鋁水平與細胞因子IL-10(r=0.072,P=0.4290.05)、IFN-γ(r=0.480,P=0.598)間未見相關性。5、IgG、IgA、IgM、IgE水平在高鋁暴露地區(qū)AA組與非高鋁暴露地區(qū)AA組間的表達均無差異(均P0.05)。6、高鋁暴露地區(qū)AA患者補體C4水平顯著低于非高鋁暴露AA組(P0.01),補體C3水平在兩組間比較無明顯統(tǒng)計學差異(P0.05)。血清鋁與補體C4水平(r=0.097,P=0.311)未見相關性。結(jié)論:高鋁暴露地區(qū)人群血清鋁水平高于非高鋁暴露地區(qū)人群。高鋁暴露可能影響AA患者免疫功能。高鋁暴露地區(qū)AA免疫細胞功能特點有別于非高鋁暴露地區(qū)AA,以細胞免疫和免疫因子改變顯著,血清鋁水平與免疫細胞和免疫因子間未見相關性。
[Abstract]:Aim: to study the changes of immune cell function and its clinical characteristics in patients with aplastic anemia (aplastic anemia,AA) exposed to high aluminum, and to analyze the relationship between serum aluminum levels and immune cells and immune factors. Methods: 62 cases of AA were selected and divided into two groups according to the aluminum content of drinking water and the source of the cases. The drinking water aluminum content was 300 渭 g / L and lived for a long time in the high aluminum exposed area of AA group (33 cases). Drinking water aluminum content 300ug/L and long-term living in non-aluminum industrial areas are non-high aluminum exposed areas of AA group, a total of 29 cases, and high aluminum exposure areas and non-high aluminum exposure areas 30 healthy people as the control group. The serum aluminum level was measured by inductively coupled plasma mass spectrometry (inductivity coupled plasma mass spectrometry,ICP-MS), and the expression of cytokines IL-10,IL-12,IL-17 and INF- 緯 was detected by enzyme-linked immunosorbent assay (enzyme linked immuno sorbent assay,ELISA). Flow cytometry (flow cytometry,FCM) was used to detect the proportion of lymphocyte subsets. The data of immunoglobulin, complement C3, C4 expression and clinical data were collected. To analyze the changes of immune cells and immune factors in AA patients exposed to high aluminum, and to explore the relationship between serum aluminum and immune indexes. Results: 1. The level of serum aluminum in high aluminum area (high aluminum AA and high aluminum healthy group) was higher than that in non high aluminum area population (non high aluminum AA and non high aluminum health group) (P0.05). The level of serum aluminum in AA patients in high aluminum exposure area was significantly higher than that in non high aluminum exposed area AA group and healthy control group (P0.01). 2. The anemia degree of AA patients in high aluminum exposed area was significantly higher than that in non high aluminum exposed area (P < 0 01). The degree of bone marrow hemopoietic tissue decrease was more severe than that in AA group (P0.05), but the age, sex, leukocyte, neutrophil, reticulocyte and platelet count in the two groups were significantly higher than those in the control group (P0.05). There was no significant difference in myeloproliferative degree. 3. The ratio of CD4 T lymphocytes and CD4 / CD8 in AA patients exposed to high aluminum were higher than those in AA group and healthy control group in non-aluminum exposed area (P 0.05). The percentage of CD8 T lymphocyte was lower than that of AA group and healthy control group (all P0.05), and there was no significant difference in CD3, CD 19, NK cell ratio between the two groups (P0.05). CD4 T, CD8 T lymphocyte ratio was not significantly different between the two groups (P 0.05). There was no correlation between CD4 / CD8 and serum aluminum level (r = 0.169, P = 0.067). 0. 072, 0. 438, 0. 072, 0. 438; (4) the level of IL-10,IFN- 緯 in AA patients exposed to high aluminum was higher than that in AA patients without high aluminum exposure (P 0.05). There was no significant difference in IL-17,IL-12 level between the two groups (P 0.05). There was no correlation between serum Al level and cytokines IL-10 (r = 0.072, P = 0.4290.05) and IFN- 緯 (r = 0.480, P = 0.598). There was no significant difference in the expression of IgE between the AA group and the AA group in the high aluminum exposure area (P0.05). 6. The complement C4 level of the AA patients in the high aluminum exposed area was significantly lower than that in the non high aluminum exposed AA group (P0.01). There was no significant difference in complement C3 level between the two groups (P0.05). There was no correlation between serum aluminum and complement C4 (r = 0.097, P = 0.311). Conclusion: the level of serum aluminum in high aluminum exposed area is higher than that in non high aluminum exposed area. High aluminum exposure may affect immune function in patients with AA. The functional characteristics of AA immune cells in high aluminum exposed areas were significantly different from those in non high aluminum exposed areas. The changes of cellular immunity and immune factors were significant in AA, exposed to high aluminum. There was no correlation between serum aluminum levels and immune cells and immune factors.
【學位授予單位】：右江民族醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R556.5
[Abstract]:Aim: to study the changes of immune cell function and its clinical characteristics in patients with aplastic anemia (aplastic anemia,AA) exposed to high aluminum, and to analyze the relationship between serum aluminum levels and immune cells and immune factors. Methods: 62 cases of AA were selected and divided into two groups according to the aluminum content of drinking water and the source of the cases. The drinking water aluminum content was 300 渭 g / L and lived for a long time in the high aluminum exposed area of AA group (33 cases). Drinking water aluminum content 300ug/L and long-term living in non-aluminum industrial areas are non-high aluminum exposed areas of AA group, a total of 29 cases, and high aluminum exposure areas and non-high aluminum exposure areas 30 healthy people as the control group. The serum aluminum level was measured by inductively coupled plasma mass spectrometry (inductivity coupled plasma mass spectrometry,ICP-MS), and the expression of cytokines IL-10,IL-12,IL-17 and INF- 緯 was detected by enzyme-linked immunosorbent assay (enzyme linked immuno sorbent assay,ELISA). Flow cytometry (flow cytometry,FCM) was used to detect the proportion of lymphocyte subsets. The data of immunoglobulin, complement C3, C4 expression and clinical data were collected. To analyze the changes of immune cells and immune factors in AA patients exposed to high aluminum, and to explore the relationship between serum aluminum and immune indexes. Results: 1. The level of serum aluminum in high aluminum area (high aluminum AA and high aluminum healthy group) was higher than that in non high aluminum area population (non high aluminum AA and non high aluminum health group) (P0.05). The level of serum aluminum in AA patients in high aluminum exposure area was significantly higher than that in non high aluminum exposed area AA group and healthy control group (P0.01). 2. The anemia degree of AA patients in high aluminum exposed area was significantly higher than that in non high aluminum exposed area (P < 0 01). The degree of bone marrow hemopoietic tissue decrease was more severe than that in AA group (P0.05), but the age, sex, leukocyte, neutrophil, reticulocyte and platelet count in the two groups were significantly higher than those in the control group (P0.05). There was no significant difference in myeloproliferative degree. 3. The ratio of CD4 T lymphocytes and CD4 / CD8 in AA patients exposed to high aluminum were higher than those in AA group and healthy control group in non-aluminum exposed area (P 0.05). The percentage of CD8 T lymphocyte was lower than that of AA group and healthy control group (all P0.05), and there was no significant difference in CD3, CD 19, NK cell ratio between the two groups (P0.05). CD4 T, CD8 T lymphocyte ratio was not significantly different between the two groups (P 0.05). There was no correlation between CD4 / CD8 and serum aluminum level (r = 0.169, P = 0.067). 0. 072, 0. 438, 0. 072, 0. 438; (4) the level of IL-10,IFN- 緯 in AA patients exposed to high aluminum was higher than that in AA patients without high aluminum exposure (P 0.05). There was no significant difference in IL-17,IL-12 level between the two groups (P 0.05). There was no correlation between serum Al level and cytokines IL-10 (r = 0.072, P = 0.4290.05) and IFN- 緯 (r = 0.480, P = 0.598). There was no significant difference in the expression of IgE between the AA group and the AA group in the high aluminum exposure area (P0.05). 6. The complement C4 level of the AA patients in the high aluminum exposed area was significantly lower than that in the non high aluminum exposed AA group (P0.01). There was no significant difference in complement C3 level between the two groups (P0.05). There was no correlation between serum aluminum and complement C4 (r = 0.097, P = 0.311). Conclusion: the level of serum aluminum in high aluminum exposed area is higher than that in non high aluminum exposed area. High aluminum exposure may affect immune function in patients with AA. The functional characteristics of AA immune cells in high aluminum exposed areas were significantly different from those in non high aluminum exposed areas. The changes of cellular immunity and immune factors were significant in AA, exposed to high aluminum. There was no correlation between serum aluminum levels and immune cells and immune factors.
【學位授予單位】：右江民族醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R556.5
【參考文獻】
相關期刊論文 前10條
1 王文松;錢美華;王曼玲;楊天新;藍建平;;再生障礙性貧血與T細胞亞群及調(diào)節(jié)性T細胞的相關性分析[J];中華全科醫(yī)學;2016年09期
2 劉春燕;邵宗鴻;;再生障礙性貧血發(fā)病機制研究進展[J];中國實用內(nèi)科雜志;2016年05期
3 張紅霞;吳廣勝;郭蔚玲;;再生障礙性貧血患者血清Th1/Th2細胞及相關細胞因子和T細胞亞群變化及意義[J];海南醫(yī)學院學報;2016年10期
4 沈英英;王博;林圣云;;再生障礙性貧血免疫損傷機制及相關淋巴細胞的研究[J];黑龍江中醫(yī)藥;2016年01期
5 王利民;王平;劉善鳳;常盼盼;胡麗華;;SLE患者血清IL-17、IL-6相關性研究[J];臨床血液學雜志(輸血與檢驗);2015年06期
6 張立;王麗;李建平;楊曉;王蒙蒙;蔡國旗;徐彬;徐勝前;劉萍;潘發(fā)明;;IL-12和IL-17強直性脊柱炎患者的外周血表達水平及兩者之間相關性研究[J];安徽醫(yī)科大學學報;2015年12期
7 陸翔;唐乾利;王小超;姜艷;邱麗芳;李，
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