【摘要】：目的采用同型半胱氨酸(Hcy)孵育體外培養(yǎng)的人臍靜脈內(nèi)皮細(xì)胞(HUVEC),建立血管內(nèi)皮細(xì)胞炎性損傷模型,后用Diplacone加以干預(yù),觀察Diplacone對HUVEC的影響,探討Diplacone對血管內(nèi)皮細(xì)胞可能的保護(hù)作用。方法先用不同濃度的Hcy預(yù)處理HUVEC,選出Hcy的最適損傷濃度(2 mmol/L)和時間(12 h),在HUVEC培養(yǎng)基中加入不同濃度Diplacone預(yù)處理2 h后加入2 mmol/L Hcy作用12 h,通過MTT法檢測細(xì)胞活性變化,Hoechst33342核染色檢測細(xì)胞核損傷程度,Western blot檢測與細(xì)胞損傷有關(guān)的血管細(xì)胞黏附分子1(VCAM-1)和P-選擇素的表達(dá)水平,以此衡量Diplacone對Hcy誘導(dǎo)的HUVEC損傷的保護(hù)作用。結(jié)果與對照組相比,2 mmol/L Hcy組細(xì)胞活性顯著降低(P0.01),10μmol/L以內(nèi)Diplacone具有明顯的劑量依賴性抑制2 mmol/L Hcy誘導(dǎo)的HUVEC活性損傷并抑制Hcy所致的VCAM-1和P-選擇素的表達(dá)升高(P0.01)。結(jié)論 Diplacone對Hcy所致的HUVEC內(nèi)皮損傷具有保護(hù)作用。
[Abstract]:Objective to establish vascular endothelial cell inflammatory injury model by incubating human umbilical vein endothelial cells (HUVEC),) with homocysteine (Hcy) in vitro, and then to observe the effect of Diplacone on HUVEC by Diplacone intervention. To investigate the possible protective effect of Diplacone on vascular endothelial cells. Methods the optimal damage concentration (2 mmol/L) and time (12 h),) of Hcy were obtained by pretreatment of HUVEC, with different concentrations of Hcy for 2 h and 12 h after pretreatment with different concentrations of Diplacone in HUVEC medium. The changes of cell activity were detected by MTT and the expression of vascular cell adhesion molecule 1 (VCAM-1) and P- selectin were detected by Hoechst33342 nuclear staining, and the degree of nuclear damage was detected by, Western blot. The protective effect of Diplacone on HUVEC damage induced by Hcy was evaluated. Results compared with the control group, the cell activity of 2 mmol/L Hcy group was significantly decreased (P0.01). Within 10 渭 mol/L, Diplacone significantly inhibited HUVEC activity damage induced by 2 mmol/L Hcy and increased VCAM-1 and P- selectin expression induced by Hcy in a dose-dependent manner (P0.01). Conclusion Diplacone has protective effect on HUVEC endothelial injury induced by Hcy.
【作者單位】： 濟(jì)南大學(xué)山東省醫(yī)學(xué)科學(xué)院醫(yī)學(xué)與生命科學(xué)學(xué)院;山東省醫(yī)學(xué)科學(xué)院藥物研究所;國家衛(wèi)生部生物技術(shù)藥物重點實驗室;山東省罕少見病重點實驗室;
【基金】：山東省自主創(chuàng)新及成果轉(zhuǎn)化專項(201402103) 山東省醫(yī)學(xué)科學(xué)院醫(yī)藥衛(wèi)生科技創(chuàng)新工程 山東省醫(yī)學(xué)科學(xué)院2015年計劃項目(2015-08)
【分類號】：R54
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本文編號：2378235