【摘要】：目的:檢測大鼠滿性心肌梗死所致心絞痛之后,內(nèi)側(cè)前額葉皮質(zhì)(medial prefrontal cortex,mPFC)錐體細胞突觸傳遞活性的變化,探討調(diào)節(jié)慢性心絞痛可能的中樞機制。方法:雄性成年SD大鼠隨機分為假手術(shù)組和慢性心肌梗死(chronic myocardial infarction,CMI)手術(shù)組,每組5只。手術(shù)2周后,制備mPFC區(qū)域急性腦片,通過全細胞膜片鉗方法記錄錐體細胞的興奮性和抑制性突觸電流,比較假手術(shù)組和手術(shù)組的突觸電流活性變化。結(jié)果:相較于假手術(shù)組動物,手術(shù)組動物mPFC內(nèi)興奮性突觸電流傳遞明顯增強,抑制性突觸電流傳遞明顯減弱。同時抑制性中間神經(jīng)元動作電位釋放下降。結(jié)論:CMI之后mPFC內(nèi)錐體細胞的興奮性突觸信號傳遞增強,可能是由于局部抑制性神經(jīng)元活性降低所致。鑒于mPFC內(nèi)錐體細胞活性對于鎮(zhèn)痛具有正向效應(yīng),這種變化對于mPFC對慢性心絞痛的中樞抑制可能具有積極的意義。
[Abstract]:Aim: to investigate the changes of synaptic transmission activity of pyramidal cells in medial prefrontal cortex (medial prefrontal cortex,mPFC) after angina pectoris induced by full myocardial infarction in rats, and to explore the possible central mechanism of regulating chronic angina pectoris. Methods: male adult SD rats were randomly divided into sham-operated group and chronic myocardial infarction (chronic myocardial infarction,CMI) group with 5 rats in each group. After 2 weeks of operation, acute brain slices of mPFC region were prepared. Excitatory and inhibitory synaptic currents of pyramidal cells were recorded by whole-cell patch clamp method, and the changes of synaptic current activity in sham operation group and operation group were compared. Results: compared with sham operation group, excitatory synaptic current transmission in mPFC was significantly increased and inhibitory synaptic current transmission was significantly decreased in operation group. At the same time, inhibitory action potential release of intermediate neurons decreased. Conclusion: the increase of excitatory synaptic signal transduction in pyramidal cells of mPFC after CMI may be due to the decreased activity of local inhibitory neurons. In view of the positive effect of pyramidal cell activity in mPFC on analgesia, this change may have a positive effect on the central inhibition of chronic angina pectoris by mPFC.
【作者單位】： 西安醫(yī)學(xué)院公共衛(wèi)生系心理學(xué)教研室;
【基金】：陜西省自然科學(xué)基礎(chǔ)研究計劃-青年項目(2017JQ8058) 陜西省教育廳科學(xué)研究項目(2017) 陜西省大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計劃平臺(201711840024) 西安醫(yī)學(xué)院博士科研啟動基金項目(2016DOC10)
【分類號】：R542.22
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本文編號：2317739