【摘要】：目的:觀察心肌梗死患者溶栓前后體內Th17、Treg細胞及相關因子含量,從而探討三者的相關性。方法:提取外周血單個核細胞,流式細胞術檢測Th17及Treg含量,ELISA方法檢測上清IL-17及TGF-β含量變化,實時定量PCR檢測RORγT及Foxp3含量。結果:與對照組相比,實驗組患者在溶栓前、后體內Th17細胞,IL-17及RORγT含量均顯著升高;Treg細胞、TGF-β及Foxp3含量顯著降低(P0.05)。與實驗組患者溶栓前相比,溶栓后7 d體內Th17細胞,IL-17及RORγT含量顯著降低;Treg細胞、TGF-β及Foxp3含量顯著升高(P0.05)。結論:與對照組相比,心肌梗死患者體內中存在Th17及相關因子升高,Treg及相關因子降低,溶栓治療后可使Th17及相關因子降低,Treg及相關因子升高。
[Abstract]:Aim: to investigate the correlation of Th17,Treg cells and related factors before and after thrombolysis in patients with myocardial infarction. Methods: peripheral blood mononuclear cells were extracted, Th17 and Treg contents were detected by flow cytometry, IL-17 and TGF- 尾 contents in supernatant were detected by Elisa, ROR 緯 T and Foxp3 contents were detected by real-time quantitative PCR. Results: compared with the control group, the contents of IL-17 and ROR 緯 T of Th17 cells in the experimental group were significantly increased before and after thrombolytic therapy (P0.05). The levels of IL-17 and ROR 緯 T in Th17 cells were significantly decreased after 7 days of thrombolysis compared with those before thrombolysis in the experimental group (P0.05). Conclusion: compared with the control group, Th17 and related factors increased and Treg decreased in patients with myocardial infarction. After thrombolytic therapy, Th17 and related factors decreased and Treg and related factors increased after thrombolytic therapy.
【作者單位】： 廈門大學附屬第一醫(yī)院杏林院區(qū)內科;廈門大學附屬第一醫(yī)院檢驗科;廈門大學附屬第一醫(yī)院普外科;廈門大學附屬第一醫(yī)院超聲影像科;
【分類號】：R542.22

【相似文獻】

相關期刊論文 前10條

1 李鴻佳;王淑娟;董亮;;Th17與慢性氣道炎癥性疾病[J];國際內科學雜志;2007年10期

2 余嫻;程翔;廖玉華;丁英俊;付卿卿;謝江嬌;姚瑞;陳勇;唐婷婷;;急性冠狀動脈綜合征患者Th17細胞檢測及意義[J];臨床心血管病雜志;2008年02期

3 袁t，

本文編號：2200263