本文選題：乙醛脫氫酶2 + 基因多態(tài)性��； 參考：《山東大學(xué)》2015年碩士論文

【摘要】：研究目的1、研究中國漢族人群乙醛脫氫酶2(aldehydedehydrogenase2, ALDH2)基因多態(tài)性與主動脈夾層的相關(guān)性。2、探討影響主動脈夾層發(fā)病以及住院期間主動脈夾層患者死亡的危險因素。研究方法采用病例-對照研究方法。入選研究對象中病例組為2014年5月～2015年5月間山東大學(xué)齊魯醫(yī)院收治的主動脈夾層患者51例,根據(jù)年齡、性別構(gòu)成比以1：2比例選取非主動脈夾層對照組102例,根據(jù)住院病歷獲得研究對象的臨床基線資料,包括年齡、性別、吸煙史、飲酒史、高血壓病史、糖尿病病史、D-Di(D-二聚體)水平、血脂水平、主動脈綜合征家族史、臨床結(jié)局等項目,血脂水平包括總膽固醇(total cholesterol, TC)、甘油三酯(triglyceride, TG)、低密度脂蛋白膽固醇(low-density lipoprotein cholesterol, LDL-C)、高密度脂蛋白膽固醇(high-density lipoprotein cholesterol, HDL-C)。抽取主動脈夾層患者及對照組清晨空腹外周肘靜脈血2m1,提取DNA,以PCR方法擴(kuò)增目的基因,所得產(chǎn)物純化后以基因測序的方法確定ALDH2基因型。根據(jù)臨床結(jié)局將病例組分為死亡組、及非死亡(好轉(zhuǎn)出院或轉(zhuǎn)院)組。SPSS18軟件進(jìn)行數(shù)據(jù)處理,對兩組間臨床基線資料、ALDH2基因多態(tài)性等進(jìn)行比較分析,采用Logistic多元回歸分析校正混雜因素,計算比值比(odds ratio, OR)及其95%置信區(qū)間(95%Confidence Interval,95%CI)。結(jié)果1.主動脈夾層組與對照組比較,性別、年齡、吸煙史無明顯統(tǒng)計學(xué)差異,而ALDH2野生型基因、高血壓病病史水平均高于對照組(p0.05),糖尿病病史、血脂異常水平低于對照組(p0.05)。2.Logistic多元回歸校正年齡、性別、糖尿病、高血壓、吸煙史、飲酒史、血脂異常等因素后,野生型ALDH2基因患者發(fā)生主動脈夾層的OR值為2.72(95%置信區(qū)間為1.16～6.41)。3.死亡亞組與非死亡亞組間性別糖尿病、吸煙史、飲酒史、心血管病家族史、血脂等反面差異均無統(tǒng)計學(xué)意義(p0.05),而D-Di、主動脈夾層類型存在顯著差異(p0.05)。結(jié)論1、野生型ALDH2基因(ALDH2*1/*1)是中國漢族人群主動脈夾層發(fā)病的危險因素。2、D-Di、主動脈夾層類型與主動脈夾層臨床結(jié)局相關(guān)。
[Abstract]:Objective 1 to study the association of aldehydes dehydrogenase 2 (ALDH2) gene polymorphism with aortic dissection in Chinese Han population, and to explore the risk factors influencing the incidence of aortic dissection and the death of patients with aortic dissection during hospitalization. Methods A case-control study was used. Fifty-one patients with aortic dissection were selected from Qilu Hospital of Shandong University from May 2014 to May 2015. 102 cases of non-aortic dissection control group were selected according to age and sex composition ratio of 1:2. The clinical baseline data including age, sex, smoking history, alcohol consumption history, hypertension history, diabetes history (D-dimer) level, blood lipid level, family history of aortic syndrome were obtained. For clinical outcomes, blood lipid levels included total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), high density lipoprotein cholesterol (HDL-C). Two m1s of fasting peripheral venous blood were extracted from patients with aortic dissection and control group in the morning. The target gene was amplified by PCR. The ALDH2 genotypes were determined by gene sequencing. According to the clinical outcome, the patients were divided into death group and non-dead group. SPSS18 software was used to process the data. The clinical baseline data of the two groups were compared and analyzed, such as the polymorphism of ALDH2 gene. Logistic multivariate regression analysis was used to correct the confounding factors and to calculate odds of odds (ORs) and 95% confidence interval (95% confidence interval). Result 1. There was no significant difference in sex, age, smoking history between aortic dissection group and control group, but ALDH2 wild type gene and hypertension history were higher than those in control group (P 0.05). The level of dyslipidemia was lower than that of control group (P < 0.05). 2. After adjusted age, sex, diabetes, hypertension, smoking history, drinking history and dyslipidemia, the OR value of aortic dissection in patients with wild-type ALDH2 gene was 2.7295% confidence interval was 1.16 / 6.41n.3. There was no significant difference in gender diabetes, smoking history, drinking history, family history of cardiovascular disease and blood lipid between the death subgroup and the non-dead subgroup, but there was significant difference in D-Diand aortic dissection type. Conclusion 1. Wild type ALDH2 gene ALDH2 1 / 1) is a risk factor for aortic dissection in Chinese Han population. The type of aortic dissection is related to the clinical outcome of aortic dissection.
【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R543.1

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本文編號：2017925