發(fā)布時(shí)間：2018-04-21 02:39

  本文選題：超聲波 + 輻照　； 參考：《遵義醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:觀察不同劑量超聲波輻照對(duì)蛙腿骨骼肌力學(xué)狀態(tài)的影響;量化評(píng)價(jià)超聲波輻照誘導(dǎo)蛙腿骨骼肌力學(xué)變化的特征;應(yīng)用組織多普勒超聲成像觀察超聲波輻照對(duì)蛙腿骨骼肌電機(jī)械興奮性及收縮性的影響。方法:(1)制備60個(gè)蛙離體坐骨神經(jīng)-腓腸肌標(biāo)本,分為A、A’、B、B’四組(15只/組),其中A、B為實(shí)驗(yàn)組,A’、B’分別為A、B的空白對(duì)照組。實(shí)驗(yàn)組接受不同超聲波輻照劑量(A組輻照劑量為3.0W/cm2、90s,B組輻照劑量為1.0W/cm2、60s)。將Powerlab多道生理信號(hào)采集處理系統(tǒng)與標(biāo)本連接,發(fā)放電刺激坐骨神經(jīng)誘導(dǎo)腓腸肌收縮,在基礎(chǔ)狀態(tài)張力數(shù)據(jù)采集后進(jìn)行超聲波輻照,在輻照后第0、第5、第10及第20分鐘重復(fù)進(jìn)行張力數(shù)據(jù)采集。評(píng)價(jià)以上各組張力測(cè)值的時(shí)間變化規(guī)律以及組間差異。(2)另行制備36個(gè)蛙離體坐骨神經(jīng)-腓腸肌標(biāo)本,分組(A組和B組各10只/組,A’組和B’組各8只/組)、干預(yù)措施及觀察時(shí)間點(diǎn)同上。所有標(biāo)本連接Powerlab多道生理信號(hào)采集處理系統(tǒng),同時(shí)通過(guò)導(dǎo)聯(lián)線與彩超儀心電通道連接以輸入同步電刺激信號(hào)。Powerlab發(fā)放電脈沖刺激坐骨神經(jīng)誘導(dǎo)腓腸肌收縮。應(yīng)用M型彩色組織多普勒及頻譜多普勒組織顯像同步觀察標(biāo)本腓腸肌在干預(yù)前后各個(gè)觀察時(shí)間點(diǎn)的電機(jī)械延遲時(shí)間(t1)、機(jī)械收縮持續(xù)時(shí)間(t2)及收縮速度(v)。分析評(píng)價(jià)以上各組三個(gè)參數(shù)的時(shí)間變化規(guī)律及組間差異。結(jié)果:(1)A組和A’組張力測(cè)值差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);但交互效應(yīng)顯著(P0.05),對(duì)照組A’張力隨著時(shí)間延長(zhǎng)而減低,實(shí)驗(yàn)組A張力隨著時(shí)間延長(zhǎng)呈增加趨勢(shì)。組內(nèi)比較,A組第5分鐘張力較基礎(chǔ)狀態(tài)增高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。B組和B’組各觀察時(shí)間點(diǎn)張力測(cè)值差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);B組與B’組張力均隨時(shí)間延長(zhǎng)而減低。(2)○1 t1:A組和A’組時(shí)間效應(yīng)、交互效應(yīng)及分組效應(yīng)差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);B組和B’組時(shí)間效應(yīng)差異有統(tǒng)計(jì)學(xué)意義(P0.05),交互效應(yīng)及分組效應(yīng)差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。○2 t2:A組和A’組間交互效應(yīng)及時(shí)間效應(yīng)差異有統(tǒng)計(jì)學(xué)意義,A’組較A組隨時(shí)間延長(zhǎng)t2呈明顯增加趨勢(shì)(P0.05),分組效應(yīng)差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);B組和B’組時(shí)間效應(yīng)、交互效應(yīng)及分組效應(yīng)差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)�！�3 v:A組和A’組間時(shí)間效應(yīng)差異有統(tǒng)計(jì)學(xué)意義,交互效應(yīng)及分組效應(yīng)差異無(wú)統(tǒng)計(jì)學(xué)意義;B組和B’組時(shí)間效應(yīng)、交互效應(yīng)及分組效應(yīng)均差異無(wú)統(tǒng)計(jì)學(xué)意義。所有組別t1、t2測(cè)值均隨時(shí)間延長(zhǎng)呈增加趨勢(shì),v測(cè)值隨時(shí)間延長(zhǎng)呈降低趨勢(shì)。結(jié)論:當(dāng)超聲波輻照強(qiáng)度為3.0W/cm2、輻照時(shí)間90s時(shí),對(duì)蛙腿骨骼肌張力有一定的正性肌力作用,輻照后第5分鐘肌力明顯增高。本研究采用的超聲波輻照條件對(duì)電機(jī)械延遲時(shí)間、機(jī)械收縮持續(xù)時(shí)間和收縮速度無(wú)顯著影響,空白對(duì)照組(A’組)較實(shí)驗(yàn)組(A組,輻照強(qiáng)度為3.0W/cm2、輻照時(shí)間90s)t2增加趨勢(shì)更明顯,間接表明超聲波輻照對(duì)標(biāo)本可產(chǎn)生一定正性作用。
[Abstract]:Objective: to observe the effects of different doses of ultrasonic irradiation on the mechanical state of frog leg skeletal muscle, and to evaluate quantitatively the characteristics of the mechanical changes of frog leg skeletal muscle induced by ultrasonic irradiation. The effects of ultrasonic irradiation on the electromechanical excitability and contraction of frog leg skeletal muscle were observed by tissue Doppler ultrasound imaging. Methods 60 isolated Frog sciatic nerve and gastrocnemius muscle specimens were prepared. The experimental group received different doses of ultrasound irradiation. The radiation dose of group A was 3.0 W / cm ~ (2) ~ (90) s ~ (-1). The dose of group B was 1.0 W / cm ~ (2 +) ~ (60) s ~ (-1). The Powerlab multi-channel physiological signal acquisition and processing system was connected with the specimen, and the sciatic nerve was stimulated to induce the contraction of gastrocnemius muscle. Tension data acquisition was repeated at 0, 5, 10 and 20 minutes after irradiation. To evaluate the time variation of tension values and the differences between groups, 36 isolated frog sciatic nerve and gastrocnemius muscle specimens were prepared. Group A and group B were divided into two groups: group A (n = 10) and group B (n = 8). All the specimens were connected to the Powerlab multi-channel physiological signal acquisition and processing system. At the same time, the synchronous electrical stimulation signal .Powerlab was used to stimulate the sciatic nerve to induce gastrocnemius muscle contraction by using the lead wire and the ECG channel of the color ultrasound instrument. M-color tissue Doppler and spectral Doppler tissue imaging were used to observe the electromechanical delay time (t _ 1), mechanical contraction duration (t _ 2) and contraction velocity of gastrocnemius muscle before and after intervention. To analyze and evaluate the time variation of the three parameters and the differences between the three groups. Results there was no significant difference in tension between group A and group A (P 0.05), but the interaction effect was significant (P 0.05). The tension of group A decreased with time, and tension of group A increased with time. The tension in group A at 5 minutes was higher than that in basic state. There was no significant difference in the observed point tension values between group B and group B (P 0.05). The tension of group B and group B 'decreased with time, and the time effect of group A and group A' decreased with the prolongation of time. There was no significant difference in the interaction effect and the group effect. There was no significant difference in the time effect between the B group and the B 'group. There was no significant difference in the interaction effect and the group effect. There was no significant difference in the interaction effect between group A and group A and the interaction effect between group A and group A' was timely. There was significant difference in the effect between group A and group A (P 0.05), but there was no significant difference between group A and group B (P 0.05), and there was no significant difference in group B and group B'in time effect between group A 'and group A (P < 0.05), but there was no significant difference between group A and group A (P < 0.05). There was no significant difference in the time effect between group A and group A, but there was no significant difference in time effect between group B and group A, but there was no significant difference in time effect between group A and group A, but there was no significant difference in time effect between group B and group B', but there was no significant difference in time effect between group A and group A. There was no significant difference in interaction effect and group effect. The values of T1 and T2 in all groups showed an increasing trend with time prolonging and the values of v showed a decreasing trend with time prolonging. Conclusion: when the intensity of ultrasound irradiation is 3.0 W / cm ~ 2 and irradiation time is 90 s, it has a positive effect on the skeletal muscle tension of frog leg, and the muscle strength of frog leg increases obviously at 5 minutes after irradiation. The ultrasonic irradiation conditions adopted in this study had no significant effect on the electromechanical delay time, mechanical contraction duration and contraction velocity. The intensity of irradiation was 3.0W / cm ~ 2, and the increasing trend of irradiation time 90s)t2 was more obvious than that of group A (blank control group). The results showed that ultrasonic irradiation had positive effect on the specimen.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R541.6;R445.1

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