本文選題：糖尿病心肌病　切入點(diǎn)：肥大細(xì)胞　出處：《中國(guó)老年學(xué)雜志》2017年18期 　論文類型：期刊論文

【摘要】：目的研究肥大細(xì)胞膜穩(wěn)定劑酮替芬對(duì)大鼠糖尿病心肌病(DCM)的影響及可能機(jī)制。方法將45只雄性Wistar大鼠隨機(jī)分為正常對(duì)照組(NC組)、DCM組和肥大細(xì)胞膜穩(wěn)定劑組(Ket組)。NC組給予普通飲食,DCM組予以高脂高糖飲食加一次性腹腔注射鏈脲佐菌素(STZ)建立DCM大鼠模型,Ket組為DCM大鼠模型建模成功后給予0.025%的酮替芬溶液1 mg/kg腹膜腔內(nèi)注射,2次/d,連續(xù)注射8 w。同時(shí),NC組和DCM組給予等體積的生理鹽水空白對(duì)照注射。實(shí)驗(yàn)完成后處死全部大鼠,剪下左心室,稱取左心室重量計(jì)算全心指數(shù)和左心室重量指數(shù),在電鏡下對(duì)各組心肌組織進(jìn)行觀察分析,應(yīng)用酶聯(lián)免疫吸附法(ELISA)檢測(cè)各組大鼠血清腫瘤壞死因子(TNF)-α、活性氧簇(ROS)表達(dá),甲苯胺藍(lán)改良染色法比較肥大細(xì)胞數(shù)量分布。結(jié)果 DCM組全心指數(shù)顯著高于NC組和Ket組(均P0.05);DCM組左心室重量指數(shù)顯著高于NC組和Ket組(均P0.05)。電鏡下NC組大鼠心肌細(xì)胞核橢圓形清晰可見,閏盤連續(xù),肌絲致密,胞內(nèi)可見大量形態(tài)結(jié)構(gòu)正常的線粒體;DCM組大鼠心肌細(xì)胞核固縮,線粒體腫脹,顯示不清晰,閏盤扭曲斷裂,肌絲溶解;Ket組大鼠心肌細(xì)胞核無明顯腫脹,核膜尚光滑、完整,無核固縮表現(xiàn),線粒體完整清晰。DCM組大鼠血清TNF-α、ROS顯著高于NC組和Ket組(均P0.05)。DCM組大鼠心肌組織中肥大細(xì)胞分布密度顯著高于NC組和Ket組。結(jié)論糖尿病大鼠心肌組織存在肥大細(xì)胞的表達(dá)增強(qiáng),肥大細(xì)胞膜穩(wěn)定劑酮替芬可減輕DCM。肥大細(xì)胞表達(dá)的增強(qiáng)可促進(jìn)TNF-α和ROS的表達(dá),部分參與DCM的發(fā)生發(fā)展。
[Abstract]:Objective to study the effect of ketotifen, a hypertrophic cell membrane stabilizer, on diabetic cardiomyopathy in rats and its possible mechanism. Methods 45 male Wistar rats were randomly divided into normal control group (NC group) and mast cell membrane stabilizer group (Ket group). DCM rat model was established by high fat and high glucose diet plus single intraperitoneal injection of streptozotocin (STZ). The Ket group was given 0.025% ketotifen solution 1 mg/kg intraperitoneal injection twice after the successful establishment of DCM rat model. At the same time, the NC group and the DCM group were given the same volume of normal saline blank control injection. After the experiment, all the rats were killed. The left ventricle was cut off, and the left ventricular weight was taken to calculate the whole heart index and the left ventricular mass index. The myocardial tissues of each group were observed and analyzed under electron microscope. The expression of tumor necrosis factor TNF- 偽 (Ros) in serum of rats in each group was detected by enzyme linked immunosorbent assay (Elisa). Results the total cardiac index in DCM group was significantly higher than that in NC group and Ket group (P 0.05). The left ventricular mass index in DCM group was significantly higher than that in NC group and Ket group (all P 0.05). The nucleus is clearly oval, The intercalated disc was continuous, the myofilament was dense, and a large number of mitochondria with normal morphology were found in DCM group. The myocardial nuclei of DCM group were constricted, mitochondria swelling, unclear, intercalated disc twisted and broken, and no obvious swelling of myocardial nucleus in Ket group. The nuclear film is still smooth, complete, nucleoless pyknosis, Compared with NC group and Ket group, the density of mast cells in myocardial tissue in DCM group was significantly higher than that in NC group and Ket group. Conclusion the expression of mast cells in myocardial tissue of diabetic rats is enhanced. Ketotifen, the stabilizer of mast cell membrane, can reduce the expression of DCM. The increased expression of mast cells can promote the expression of TNF- 偽 and ROS, and partly participate in the development of DCM.
【作者單位】： 瀘州醫(yī)學(xué)院附屬醫(yī)院內(nèi)分泌科;
【分類號(hào)】：R542.2;R587.2

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本文編號(hào)：1589128