本文關(guān)鍵詞： 原發(fā)性高血壓 利鈉肽清除受體基因 單核苷酸多態(tài)性 體重指數(shù)　出處：《寧波大學》2015年碩士論文　論文類型：學位論文

【摘要】：目的探討利鈉肽清除受體(natriuretic peptide clearance receptor,NPRC)基因(NPR3)多態(tài)性與原發(fā)性高血壓(essential hypertension,EH)的關(guān)系。方法本研按照病例-對照方法設(shè)計,收集原發(fā)性高血壓患者組452例和年齡、性別相仿的血壓正常者為對照組434例作為研究對象。對吸煙史、飲酒史、2型糖尿病病史、高血壓病病史和家族史等進行記錄。檢測血生化項目:包括總膽固醇、甘油三酯、低密度脂蛋白膽固醇、高密度脂蛋白膽固醇、空腹血糖、尿酸、肌酐、尿素氮、鈉離子和鉀離子等指標。使用標簽SNP(Tag SNP)策略選擇9個NPR3基因SNPs位點(rs3792758、rs1173773、rs13436831、rs11750438、rs1173747、rs2292026、rs976576、rs696831、rs3811953)。采用多聚酶鏈反應(yīng)(polymerase chain reaction,PCR)技術(shù)擴增NPR3基因片段DNA,經(jīng)多重SNa Pshot平臺技術(shù)SNPs基因高效擴增,利用ABI 3130基因分析儀分析基因型,運用Peak Scanner 1.1軟件分析結(jié)果。NPR3基因SNPs位點基因型在病例和對照組中的分布頻率使用Hardy-Weinberg平衡檢驗。統(tǒng)計軟件為SPSS18.0軟件,t檢驗、卡方檢驗和二元Logistic回歸分析用于數(shù)據(jù)統(tǒng)計分析,雙側(cè)檢驗,P0.05為具有統(tǒng)計學意義。應(yīng)用Haploview4.2程序作連鎖不平衡分析。結(jié)果Hardy-Weinberg平衡檢驗采用卡方檢驗,所選9個SNPs在病例組和對照組人群中P值均0.05,符合Hardy-Weinberg平衡定律。病例組與對照組NPR3基因SNPs基因型經(jīng)二元Logistic回歸分析,rs1173773GG基因型攜帶者患高血壓病的風險增加,OR值95%CI 3.164(1.011-9.903),P值0.037,具有統(tǒng)計學意義,經(jīng)調(diào)整年齡、性別、體重指數(shù)、腰圍、吸煙史、飲酒史和高血壓病家族史后,OR值95%CI 3.012(1.002-9.728),P值0.041,仍具有統(tǒng)計學意義。其他8個SNPs在兩組之間比較未發(fā)現(xiàn)患高血壓病風險,P值均0.05。兩組等位基因型比較經(jīng)卡方檢驗,P值均0.05,差異無統(tǒng)計學意義。進一步在高血壓病組內(nèi)比較,BMI"g25Kg/m2與BMI25Kg/m2相比,GG基因型攜帶者超重或者肥胖的風險更高,OR值95%CI3.509(0.935-13.176),P值0.049,具有統(tǒng)計學意義;G/A等位基因型攜帶比較,P0.05,無統(tǒng)計學意義。另外,在高血壓病組內(nèi)是否合并2型糖尿病各113名比較,G/A等位基因型攜帶分布差異無統(tǒng)計學意義。結(jié)論本研究發(fā)現(xiàn)在中國漢族人群中,NPR3基因rs1173773 GG基因攜帶者患原發(fā)性高血壓風險增加;且原發(fā)性高血壓合并超重或肥胖的風險亦增加。
[Abstract]:Objective to investigate natriuretic peptide clearance receptor. NPRC3) polymorphism was associated with essential hypertensionin essential hypertension (EH). Methods the study was designed with a case-control method. A total of 452 patients with essential hypertension and patients with normal blood pressure of similar age and sex were collected as control group 434 cases as control group. The history of smoking and drinking were compared with the history of type 2 diabetes mellitus. The history of hypertension and family history were recorded. The blood biochemical items included total cholesterol, triglyceride, low density lipoprotein cholesterol, high density lipoprotein cholesterol, fasting blood glucose, uric acid, creatinine. Urea nitrogen, sodium ion and potassium ion were used to select 9 SNPs loci of NPR3 gene, rs3792758 and rs1173773. Rs13436831,rs11750438,rs1173747,rs2292026,rs976576,rs696831. NPR3 gene fragment DNA was amplified by polymerase chain reaction (PCR) and polymerase chain reaction- PCR (PCR) technique. The SNPs gene was amplified efficiently by multiplex SNa Pshot platform, and genotypes were analyzed by ABI 3130 gene analyzer. Using Peak Scanner. 1.1 results of software analysis. The distribution frequency of SNPs locus genotype of NPR3 gene in case and control group was tested by Hardy-Weinberg equilibrium test. The statistical software was SPSS18. .0 software. T test, chi-square test and binary Logistic regression analysis were used for statistical analysis and bilateral test. P0.05 was statistically significant. Haploview4.2 program was used for linkage disequilibrium analysis. Results the Hardy-Weinberg balance test was chi-square test. The 9 SNPs were all P 0. 05 in the case group and the control group. The SNPs genotype of NPR3 gene in case group and control group was analyzed by binary Logistic regression analysis. The risk of hypertension in rs1173773GG genotype carriers was increased by 95 CI 3.164U 1.011-9.903 P value 0.037. After adjusting for age, sex, body mass index, waist circumference, smoking history, drinking history and family history of hypertension, OR was 95 CI 3.0121.002-9.728). P value was 0. 041, which was still statistically significant. The other 8 SNPs were not found in the two groups. The allele genotypes of the two groups were compared with each other by chi-square test. There was no significant difference in P value (P = 0.05). Further comparison was made between BMI "g _ 25kg / m ~ 2 and BMI25Kg/m2 in hypertension group." The risk of overweight or obesity in GG genotype carriers was higher than that in GG genotype carriers. The OR value of GG genotype carriers was 95CI3.509 ~ 0.935-13.176 (P = 0.049), which was statistically significant. There was no significant difference in the G / A allele carrying rate (P 0.05). In addition, 113 patients with type 2 diabetes were compared in the hypertension group whether or not they were associated with type 2 diabetes. There was no significant difference in the distribution of G / A allele genotypes. Conclusion this study found that G- A alleles were found in Chinese Han population. The risk of essential hypertension was increased in NPR3 rs1173773 GG carriers. The risk of essential hypertension associated with overweight or obesity also increased.
【學位授予單位】：寧波大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R544.11

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