【摘要】：目的觀察鹽酸小檗堿對高脂飲食誘導的非酒精性脂肪性肝病(NAFLD)大鼠腸道菌群的影響。方法 18只雄性SD大鼠隨機分為普通飼料喂養(yǎng)組(正常對照組)、高脂飲食誘導NAFLD組(NAFLD組)和高脂飲食誘導NAFLD小檗堿干預組(小檗堿干預組),每組6只。采用16SrRNA序列分析法研究3組大鼠腸道菌群的變化;觀察3組大鼠肝臟和回腸組織形態(tài)學改變。結果 3組大鼠的腸道菌群主要分布在擬桿菌門和厚壁菌門;與正常對照組比較,NAFLD組的厚壁菌門比例顯著增高(P0.05),擬桿菌門比例及有益菌乳酸菌屬比例則顯著降低(P0.01);與NAFLD組比較,小檗堿干預組的厚壁菌門比例顯著下降(P0.01),擬桿菌門比例和有益菌乳酸菌屬比例顯著增高(P0.01)。肝臟組織學檢查發(fā)現(xiàn):正常對照組大鼠肝臟細胞排列正常,未見脂肪滴和炎癥細胞浸潤;NAFLD組大鼠肝臟組織出現(xiàn)大量的脂肪變性,肝細胞排列較正常對照組紊亂;小檗堿干預組大鼠肝臟組織仍有脂肪變性,但脂肪變性程度較NAFLD組減輕�；啬c組織學檢查發(fā)現(xiàn):正常對照組大鼠回腸絨毛排列整齊、緊湊,表面結構完整;NAFLD組大鼠回腸絨毛出現(xiàn)斷裂、缺失,間隙增寬;小檗堿干預組大鼠回腸絨毛排列較為整齊,斷裂、缺失較NAFLD組少。結論小檗堿可以改善高脂飲食所誘導的NAFLD,其機制可能與改變腸道菌群的結構及減輕高脂飲食導致的腸道黏膜損傷有關。
[Abstract]:Aim to observe the effect of berberine hydrochloride on intestinal flora in (NAFLD) rats with nonalcoholic fatty liver disease induced by high fat diet. Methods 18 male SD rats were randomly divided into three groups: normal diet group (normal control group), high fat diet induced NAFLD group (NAFLD group) and high fat diet induced NAFLD berberine intervention group (berberine intervention group), with 6 rats in each group. 16SrRNA sequence analysis was used to study the changes of intestinal microflora and histological changes of liver and ileum in three groups. Results the intestinal microflora of the three groups of rats was mainly distributed in the myxomycetes and the thick-walled fungi. Compared with the normal control group, the proportion of thick-walled bacteria in NAFLD group was significantly higher (P0.05), while the proportion of mycorrhizal bacteria and the proportion of beneficial lactic acid bacteria were significantly decreased (P0.01). Compared with NAFLD group, the percentage of thick-walled bacteria in berberine intervention group decreased significantly (P0.01), and the proportion of mycorrhizal bacteria and the proportion of lactic acid bacteria of beneficial bacteria increased significantly in berberine intervention group (P0.01). Liver histological examination showed that normal liver cells were arranged in normal control group, fat droplets and inflammatory cells were not seen in NAFLD group, fatty degeneration appeared in liver tissue of normal control group, hepatic cell arrangement was disordered compared with normal control group, and there was no fat drop and inflammatory cell infiltration in liver tissue of normal control group. Steatosis was found in the liver tissue of the berberine intervention group, but the degree of steatosis was less than that of the NAFLD group. The histological examination of the ileum showed that the villi of the ileum of the normal control group were arranged orderly, compact and the surface structure was intact, and the villi of the ileum of the NAFLD group were broken, missing and the gap widened. The villi of ileum of berberine intervention group were neatly arranged and broken, and the deletion was less than that of NAFLD group. Conclusion berberine can improve NAFLD, induced by high fat diet and its mechanism may be related to changing the structure of intestinal flora and reducing intestinal mucosal damage induced by high fat diet.
【作者單位】： 上海交通大學醫(yī)學院附屬第九人民醫(yī)院內分泌科;
【基金】：國家重點基礎研究發(fā)展計劃(“973”計劃)(2012CB524906)~~
【分類號】：R575.5

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