本文選題：Periostin + 蛋氨酸膽堿缺乏的飼料��； 參考：《廈門大學》2014年碩士論文

【摘要】：非酒精性脂肪肝炎是指除酒精、病毒和遺傳性等因素之外所導致的脂肪性肝炎,即肝臟中超過5%～10%的肝細胞中發(fā)生脂質累積的現(xiàn)象,并且還伴隨有炎癥和纖維化現(xiàn)象。非酒精性脂肪肝炎可以進一步惡化形成肝硬化,甚至肝癌。雖然對非酒精性脂肪肝炎的病理機制已有大量的研究,但是其發(fā)生機制仍然沒有得到確切闡明。Periostin作為一種細胞外基質蛋白,不僅在發(fā)育、組織修復、腫瘤發(fā)生發(fā)展過程中起著重要作用,而且與一些炎癥性疾病如哮喘、肺炎等也具有密切關系,但是有關Periostin在肝炎當中的作用目前還未有報道。本研究主要探討了Periostin在非酒精性脂肪肝炎中的作用。我們發(fā)現(xiàn)在MCD飼料誘導C57BL/6小鼠發(fā)生非酒精脂肪肝炎后,小鼠肝臟中Periostin的表達量升高,但Periostin基因敲除小鼠與同窩對照組相比,NASH現(xiàn)象明顯減輕。0.2mM棕櫚酸鹽處理人正常肝細胞系QSG7701發(fā)生脂質堆積現(xiàn)象；其條件培養(yǎng)基可誘導人肝臟星形細胞系LX-2表達Periostin。另外,體外過表達Periostin的QSG7701細胞在0.2mM棕櫚酸鹽處理后,其PPARy表達水平相對于對照組明顯上升。因此,這些結果表明Periostin參與了MCD飼料模型誘導的非灑精性脂肪肝炎的發(fā)生發(fā)展過程。
[Abstract]:Non-alcoholic fatty liver disease is a kind of fatty hepatitis caused by alcohol, virus and heredity, that is, lipid accumulation occurs in more than 5% of liver cells and accompanied by inflammation and fibrosis. Non-alcoholic fatty liver disease can further worsen the formation of liver cirrhosis, and even liver cancer. Although a great deal of research has been done on the pathological mechanism of non-alcoholic fatty liver disease, the pathogenesis of nonalcoholic fatty liver disease has not been clarified exactly. Periostin, as an extracellular matrix protein, is not only in development, but also in tissue repair. Tumor plays an important role in tumorigenesis and development, and is closely related to some inflammatory diseases such as asthma and pneumonia. However, the role of Periostin in hepatitis has not been reported. The purpose of this study was to investigate the role of Periostin in non-alcoholic fatty liver disease. We found that the expression of Periostin in the liver of C57BL / 6 mice increased after MCD diet induced non-alcoholic fatty liver inflammation. Compared with the control group, Periostin gene knockout mice significantly reduced lipid accumulation in human normal liver cell line QSG7701 treated with 0.2mm palmitate, and its conditioned medium could induce the expression of Periostinin in human hepatic stellate cell line LX-2. In addition, the expression level of PPARy in QSG7701 cells treated with 0.2 mm palmitate was significantly higher than that in control group. Therefore, these results suggested that Periostin was involved in the development of non-spermatogenic fatty hepatitis induced by MCD feed model.
【學位授予單位】：廈門大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R575.5

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本文編號：2075930