發(fā)布時間：2018-06-23 06:49

  本文選題：潰瘍性結腸炎 + 糞菌移植��； 參考：《第三軍醫(yī)大學學報》2017年12期

【摘要】：目的研究糞菌移植(fecal microbiota transplantation,FMT)對硫酸葡聚糖(dextran sulfate sodium,DSS)誘導的潰瘍性結腸炎小鼠的治療作用。方法將92只7周齡雌性C57BL/6小鼠,按照隨機數(shù)字表法分為4組,每組23只:對照組正常飲水,模型組飲用2%DSS 7 d,在第8天時換成不含DSS的滅菌純凈水飲用7 d,FMT預防組在飲用DSS建模的同時給予FMT灌胃處理,FMT治療組在飲用7 d DSS模型建成后進行FMT灌胃處理7 d。記錄各組小鼠體質量變化和疾病活動度,并收集每日糞便進行腸道菌群分析。在第7、14天末分別處死10只小鼠,采集血液標本進行TNF-α和D-乳酸測量,留取結腸組織分別進行HE染色、透射電鏡觀察,免疫組化染色檢測緊密連接相關蛋白ZO-1和Occludin的表達。結果 FMT治療組小鼠體質量、疾病活動程度和結腸長度較DSS誘導結腸炎小鼠模型組顯著改善(P0.01)。FMT治療組血清D-乳酸和TNF-α水平較模型組明顯降低(P0.05,0.01)。透射電鏡可見FMT治療組結腸上皮的損害及細胞間緊密連接結構和上皮完整性較模型組顯著改善。FMT治療及預防處理后ZO-1和Occludin的表達較模型組明顯增高(P0.05,P0.01)。FMT預防及治療都能顯著改善DSS引起的腸道菌群改變,促進乳桿菌屬增殖而抑制擬桿菌屬和顫螺菌屬的生長。結論FMT預防及治療都能顯著改善硫酸葡聚糖引起的腸道菌群改變,促進乳桿菌屬增殖而抑制擬桿菌屬和顫螺菌屬的生長。
[Abstract]:Objective to study the therapeutic effect of fecal bacteria transplantation of (fecal microbiota transplants on mice with ulcerative colitis induced by dextran sulfate (dextran sulfate sodium (DSS). Methods Ninety-two 7-week-old female C57BL / 6 mice were randomly divided into 4 groups, 23 in each group. The model group drank 2 DSS for 7 days. On the 8th day, the model group was replaced with sterilized water without DSS for 7 days. The prevention group was treated with FMT for 7 days after drinking DSS. Changes in body mass and disease activity were recorded and daily feces were collected for intestinal flora analysis. At the end of the 7th day, 10 mice were killed. TNF- 偽 and D- lactic acid were measured in blood samples, HE staining, transmission electron microscopy and immunohistochemical staining were used to detect the expression of tight junction protein ZO-1 and Occludin. Results the body weight, disease activity and colonic length in FMT group were significantly improved than those in DSS induced colitis model group (P0.01). The serum levels of D- lactic acid and TNF- 偽 in FMT group were significantly lower than those in model group (P0.05 + 0.01). Transmission electron microscope showed that the lesion of colonic epithelium, the structure of intercellular tight junction and the integrity of epithelium in FMT group were significantly improved compared with model group. The expression of ZO-1 and Occludin in FMT treatment group was significantly higher than that in model group (P0.05, P0.01). FMT prevention and treatment. Can significantly improve the intestinal flora changes caused by DSS, Promote the proliferation of Lactobacillus and inhibit the growth of Bacteroides and Helicobacterium. Conclusion both prevention and treatment of FMT can significantly improve the intestinal flora changes induced by dextran sulfate, promote the proliferation of Lactobacillus and inhibit the growth of Bacteroides and Helicobacterium.
【作者單位】： 第三軍醫(yī)大學大坪醫(yī)院野戰(zhàn)外科研究所消化內科;
【基金】：第三軍醫(yī)大學大坪醫(yī)院野戰(zhàn)外科研究所臨床科研基金(2014YLC05) 重慶市科委社會民生基金(CSTC2015shmszx120047)~~
【分類號】：R574.62

【相似文獻】

相關期刊論文 前10條

1 李琪毅;舒建昌;;腸黏膜屏障損傷機制研究進展[J];臨床薈萃;2006年06期

2 楊書良;席豐;郝志芳;;腸黏膜屏障損傷的診斷和治療研究進展[J];臨床薈萃;2009年04期

3 高金生;楊書良;;腸黏膜屏障損傷的原因與機制研究進展[J];世界華人消化雜志;2009年15期

4 田景華;;腸黏膜屏障的研究進展[J];醫(yī)學理論與實踐;2013年15期

5 李琴;劉立新;;腸黏膜屏障與腸源性內毒素血癥的關系研究進展[J];中華消化病與影像雜志(電子版);2012年04期

6 邱明鏈,陳思曾;免疫營養(yǎng)素在腸黏膜屏障功能障礙中的作用[J];福建醫(yī)科大學學報;2005年S1期

7 鄭盛;劉海;;腸黏膜屏障功能障礙研究進展[J];實用肝臟病雜志;2008年02期

8 李世榮;;腸黏膜屏障與抗生素相關性腸炎[J];中國醫(yī)藥導刊;2008年02期

9 席豐;高金生;楊書良;;組胺對腸黏膜屏障的保護作用[J];新醫(yī)學;2009年04期

10 徐璇;吳堅;;危重癥腸黏膜屏障功能障礙的機制研究[J];醫(yī)學綜述;2009年13期

相關會議論文 前2條

1 樊春華;呂永慧;;腸黏膜屏障功能障礙的中西醫(yī)診治研究進展[A];中華中醫(yī)藥學會脾胃病分會第二十三次全國脾胃病學術交流會論文匯編[C];2011年

2 王裴;王鳳君;;腸黏膜屏障功能的評估[A];第八屆西南五省一市燒傷整形學術會議暨貴州省醫(yī)學會燒傷整形分會2010年學術年會論文匯編[C];2010年

相關重要報紙文章 前1條

1 解放軍153醫(yī)院感染科主任 王全楚;理念更新 治肝先治腸[N];健康報;2011年

相關博士學位論文 前3條

1 劉圣p，

本文編號：2056253