發(fā)布時(shí)間：2018-06-10 09:12

  本文選題：芒果苷 + 果糖��； 參考：《第三軍醫(yī)大學(xué)學(xué)報(bào)》2015年20期

【摘要】：目的研究芒果苷改善高果糖誘導(dǎo)的Hep G2細(xì)胞內(nèi)甘油三酯沉積的可能機(jī)制。方法低糖(1 g/L葡萄糖)條件下培養(yǎng)的Hep G2細(xì)胞分為對(duì)照組(1 g/L葡萄糖,無(wú)果糖)、果糖組(1 g/L葡萄糖+30 mmol/L果糖)、果糖+芒果苷組(同時(shí)加入葡萄糖濃度1 g/L+30 mmol/L果糖+不同劑量的芒果苷,使其藥物終濃度分別為6.25、12.5、25、50μmol/L),藥物干預(yù)24 h之后,油紅O染色觀察細(xì)胞內(nèi)脂滴的沉積情況,酶法測(cè)定細(xì)胞內(nèi)甘油三酯(TG)的含量,Real-time PCR檢測(cè)碳水化合物反應(yīng)元件結(jié)合蛋白(ChREBP)、固醇調(diào)節(jié)元件結(jié)合蛋白1c(SREBP-1c)、肝型丙酮酸激酶(LPK)、二酯酰甘油�；D(zhuǎn)移酶2(DGAT-2)mRNA表達(dá)的變化。結(jié)果油紅O染色結(jié)果顯示,與對(duì)照組相比,果糖組的Hep G2細(xì)胞脂滴顯著增多,細(xì)胞內(nèi)TG含量也明顯升高(P0.05)。與果糖組對(duì)比,低劑量的芒果苷對(duì)果糖導(dǎo)致的細(xì)胞內(nèi)脂滴的沉積無(wú)影響,較高劑量的芒果苷(25μmol/L和50μmol/L)使細(xì)胞內(nèi)的脂滴數(shù)量明顯減少,細(xì)胞內(nèi)TG含量顯著降低(P0.05),以50μmol/L的芒果苷干預(yù)效果最好。Real-time PCR結(jié)果顯示,與對(duì)照組相比,果糖組Hep G2細(xì)胞ChREBP、SREBP-1c、LPK、DGAT-2 mRNA明顯升高,50μmol/L芒果苷能夠下調(diào)Hep G2細(xì)胞ChREBP、LPK、DGAT-2 mRNA的高表達(dá)(P0.05),但對(duì)SREBP-1c mRNA表達(dá)影響不明顯。結(jié)論芒果苷能夠改善高果糖誘導(dǎo)的Hep G2細(xì)胞內(nèi)TG的沉積,可能與抑制脂質(zhì)合成相關(guān)基因ChREBP、LPK、DGAT-2 mRNA的表達(dá)密切相關(guān)。
[Abstract]:Objective to study the possible mechanism of mangiferin in improving triglyceride deposition in Hep G2 cells induced by high fructose. Methods HepG2 cells cultured with low glucose (1 g / L glucose) were divided into control group (1 g / L glucose). Without fructose, the fructose group with 1 g / L glucose 30 mmol / L fructose, fructose mangiferin group (1 g / L 30 mmol / L fructose with different doses of mangiferin was added at the same time, the final drug concentration was 6.25% 12.55% 25 渭 mol / L, respectively. After 24 hours of drug intervention, Oil red O staining was used to observe the deposition of lipid droplets in cells. Real-time PCR was used to detect the expression of carbohydrate response element binding protein (CHREBPN), steroid regulatory element binding protein (1cctbbp1), hepatic pyruvate kinase (LPKK) and diester acylglyceryl transferase (2DGAT-2). Results the results of oil red O staining showed that the lipid droplets of Hep G2 cells in fructose group were significantly higher than those of the control group, and the content of TG in the cells was also significantly higher than that in the control group. Compared with fructose group, low dose mangiferin had no effect on lipid droplet deposition in cells induced by fructose, and high dose mangiferin 25 渭 mol / L and 50 渭 mol / L) significantly reduced the number of lipid droplets in cells. The content of TG in cells decreased significantly (P 0.05). Compared with the control group, 50 渭 mol / L mangiferin had the best effect on real-time PCR. Fructose treated Hep G2 cell line ChREBPnSREBP-1 / LPKP-1 / LPKDGAT-2mRNA increased by 50 渭 mol / L mangiferin could down-regulate the high expression of P0.05mRNA in Hep G2 cells, but had no significant effect on the expression of SREBP-1c mRNA. Conclusion mangiferin can improve the deposition of TG in Hep G2 cells induced by high fructose, which may be related to the inhibition of the expression of LPKN DGAT-2 mRNA in Hep G2 cells induced by high fructose.
【作者單位】： 重慶醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院干細(xì)胞與組織工程研究室;重慶醫(yī)科大學(xué)生命科學(xué)院脂糖代謝重點(diǎn)實(shí)驗(yàn)室;重慶醫(yī)科大學(xué)中醫(yī)藥學(xué)院中醫(yī)藥研究室;
【基金】：國(guó)家自然科學(xué)基金面上項(xiàng)目(81374033)~~
【分類號(hào)】：R575.5

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本文編號(hào)：2002701