本文選題：組織激肽釋放酶結合蛋白 + 肝星狀細胞�。� 參考：《藥學學報》2017年09期

【摘要】：為揭示kallistatin對肝星狀細胞氧化損傷的保護作用及相關分子機制,本文以過氧化氫和鐵過載為氧化模型,以大鼠原代肝星狀細胞及完全活化的人肝星狀細胞系LX-2為對象,研究了kallistatin在對肝星狀細胞活力、超氧化物、NADPH和Akt、e NOS等分子的影響。實驗結果顯示,kallistatin既能避免肝星狀細胞受氧化損傷,還能修復氧化應激造成的細胞損傷;其主要機制為kallistatin能清除細胞內(nèi)的超氧化物、降低細胞內(nèi)NADPH活力;另外,kallistatin還能激活Akt和e NOS等分子發(fā)揮抗氧化作用。本研究為加速肝纖維化藥物新靶點開發(fā)提供了理論依據(jù)。
[Abstract]:In order to reveal the protective effect of kallistatin on oxidative damage of hepatic stellate cells and its related molecular mechanisms, hydrogen peroxide and iron overload were used as oxidation models, and rat primary hepatic stellate cells and fully activated human hepatic stellate cell line LX-2 were used as subjects.The effects of kallistatin on the activity of hepatic stellate cells, superoxide NADPH and NOS were studied.The results showed that kallistatin could not only avoid oxidative damage of hepatic stellate cells, but also repair the oxidative stress, and its main mechanism was that kallistatin could scavenge the superoxide and decrease the activity of NADPH in the cells.In addition, kallistatin can also activate Akt, e NOS and other molecules to play an antioxidant role.This study provides a theoretical basis for accelerating the development of new drug targets for liver fibrosis.
【作者單位】： 泉州師范學院化工與材料學院;華僑大學分子藥物學研究院;
【基金】：國家自然科學基金資助項目(81502687,81371669) 福建省自然科學基金資助項目(2017J01548) 泉州市科技計劃項目(2016Z070,2016N006) 華僑大學中青年教師科技創(chuàng)新資助計劃(ZQN-PY319)
【分類號】：R575.2

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