本文選題：硫化氫 + 脂解　； 參考：《中國(guó)病理生理雜志》2017年10期

【摘要】：目的:研究硫化氫(H2S)供體GYY4137釋放的外源性H2S對(duì)小鼠原代肝細(xì)胞內(nèi)脂質(zhì)自噬分解的影響。方法:采用2步原位灌流法分離C57BL/6小鼠原代肝細(xì)胞并分為4組:用正常培養(yǎng)基培養(yǎng)正常組細(xì)胞;模型組用含1.2 mmol/L油酸(溶于10%的BSA)的培養(yǎng)基培養(yǎng)48 h;H2S組和炔丙基甘氨酸(PAG)組則用1.2 mmol/L油酸(溶于10%的BSA)的培養(yǎng)液培養(yǎng)48 h,然后換無(wú)血清無(wú)酚紅的RPMI-1640培養(yǎng)液(同時(shí)分別給予1 mmol/L GYY4137和200μmol/L PAG)處理6 h。收集各組細(xì)胞做LC3免疫熒光染色,拍攝熒光和相差圖片;Western blot檢測(cè)肝細(xì)胞中LC3-Ⅰ/Ⅱ的蛋白表達(dá)量;透射電鏡觀(guān)察肝細(xì)胞超微結(jié)構(gòu)。結(jié)果:和模型組相比,H2S組肝細(xì)胞內(nèi)LC3熒光顆粒和蛋白表達(dá)量增加,自噬溶酶體數(shù)量增加,細(xì)胞內(nèi)空泡增多。結(jié)論:外源性H2S可促進(jìn)脂肪變性肝細(xì)胞內(nèi)脂質(zhì)自噬分解。
[Abstract]:Aim: to study the effect of exogenous H _ 2S released by hydrogen sulfide (H _ 2S) donor GYY4137 on lipid autophagy in primary hepatocytes of mice.Methods: the primary hepatocytes of C57BL/6 mice were isolated by two-step in situ perfusion and divided into 4 groups: normal cells were cultured in normal culture medium;The model group was cultured with 1.2 mmol/L oleic acid (10% BSA-soluble medium) for 48 h in H2S group and propargyl glycine group (PAG) in 1.2 mmol/L oleic acid (10% BSA-soluble medium) for 48 h, and then replaced with serum-free RPMI-1640 medium (at the same time).They were treated with 1 mmol/L GYY4137 and 200 渭 mol/L PAG for 6 h, respectively.The expression of LC3- 鈪，

本文編號(hào)：1735349