【摘要】：目的：探討鼻腔滴入抗白介素5多克隆抗體（IL-5抗體）治療大鼠變應(yīng)性鼻炎（AR）的機(jī)制。 方法：采用卵白蛋白（OVA）及氫氧化鋁建立大鼠變應(yīng)性鼻炎（AR）模型，分為模型組（B組），激素組即丙酸氟替卡松組（C組），抗IL-5抗體組（D組），每組6只大鼠。模型建立后C組大鼠鼻腔噴入丙酸氟替卡松，每只每日每側(cè)鼻孔一噴,持續(xù)7天；D組大鼠每只每日每側(cè)鼻孔滴入IL-5抗體30L，持續(xù)一周；B組大鼠處死；另設(shè)陰性對(duì)照組（A組）6只，取鼻腔灌洗液及骨髓細(xì)胞懸液測(cè)定嗜酸性粒細(xì)胞百分比，黏膜切片觀察鼻黏膜組織病理學(xué)改變及GATA-3的表達(dá)。 結(jié)果：D組大鼠鼻腔灌洗液及骨髓腔沖洗液中嗜酸性粒細(xì)胞百分比明顯低于B組（P 0.01；P 0.01）；病理切片結(jié)果顯示：B組大鼠的鼻腔黏膜層增厚，并伴有大量炎性細(xì)胞浸潤(rùn)，主要以嗜酸細(xì)胞為主，且粘膜充血、水腫，纖毛稀疏。而D組大鼠的炎性癥狀改變明顯較B組減輕；免疫組化結(jié)果顯示B組大鼠鼻腔粘膜組織中GATA-3表達(dá)增加，而D組的表達(dá)減少，，D組大鼠鼻粘膜免疫組化的平均光密度值明顯低于B組（P 0.01），差異有統(tǒng)計(jì)學(xué)意義。 結(jié)論： 1.抗IL-5抗體對(duì)大鼠變應(yīng)性鼻炎具有明顯治療作用 2.抗IL-5抗體對(duì)大鼠變應(yīng)性鼻炎的治療作用在細(xì)胞水平上表現(xiàn)為保護(hù)大鼠鼻粘膜纖毛結(jié)構(gòu)，降低嗜酸性粒細(xì)胞在鼻和骨髓中的浸潤(rùn)或分布。 3.抗IL-5抗體降低嗜酸性粒細(xì)胞在鼻黏膜中的浸潤(rùn)與其顯著抑制大鼠鼻粘膜GATA-3轉(zhuǎn)錄因子的蛋白表達(dá)有關(guān)。
[Abstract]:Objective: to investigate the mechanism of anti-interleukin-5 polyclonal antibody (IL-5) in the treatment of allergic rhinitis in rats with (AR). Methods: the (AR) model of allergic rhinitis in rats was established by using ovalbumin (OVA) and aluminum hydroxide. They were divided into three groups: model group (group B), corticosteroid group (group C) and anti-IL-5 antibody group (group D) with 6 rats in each group. After the establishment of the model, fluticasone propionate was injected into nasal cavity of rats in group C for 7 days, while rats in group D were injected with IL-5 antibody 30L every day for one week, and rats in group B were killed. The percentage of eosinophils in nasal lavage fluid and bone marrow cell suspension were measured. Histopathological changes of nasal mucosa and the expression of GATA-3 were observed by mucosal sections. Results: the percentage of eosinophils in nasal lavage fluid and bone marrow lavage fluid in group D was significantly lower than that in group B (P 0.01? P 0.01). Pathological sections showed that the nasal mucosa of group B was thickened, accompanied by a large number of inflammatory cell infiltration, mainly eosinophils, mucosal congestion, edema, sparse cilia. The changes of inflammatory symptoms in group D were significantly less than those in group B; The results of immunohistochemistry showed that the expression of GATA-3 in nasal mucosa of group B was increased while that of group D was decreased. The average optical density of nasal mucosa of group D was significantly lower than that of group B (P 0.01). Conclusions: 1. Anti-IL-5 antibody has obvious therapeutic effect on allergic rhinitis in rats. The therapeutic effect of anti-IL-5 antibody on allergic rhinitis in rats was shown to protect the ciliated structure of the nasal mucosa and decrease the infiltration or distribution of eosinophils in the nasal and bone marrow at the cellular level. 3. The decrease of eosinophils infiltration in nasal mucosa by anti-IL-5 antibody is related to its significant inhibition of the protein expression of GATA-3 transcription factor in nasal mucosa of rats.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R765.21

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