【摘要】：目的：氣道炎癥是涉及多種炎癥細胞和炎癥介質(zhì)的慢性免疫性疾病,目前對氣道炎癥的治療不理想,因此對其發(fā)病機制的進一步研究以及尋找新的藥物治療、預(yù)防疾病是非常有意義的。CRF在外周炎癥中發(fā)揮重要的作用,本試驗的目的是通過研究CRF在上氣道炎癥患者鼻組織中的表達情況以及與嗜酸性粒細胞、肥大細胞的相關(guān)性,探討CRF在氣道炎癥性疾病中的作用。方法：標本均來自住院行鼻內(nèi)鏡手術(shù)的患者：變應(yīng)性鼻炎合并鼻息肉組10例、變應(yīng)性鼻炎組9例和正常對照組7例,鼻息肉組收集鼻息肉標本,變應(yīng)性鼻炎組和正常對照組則收集下鼻甲標本。行免疫組化染色和圖像分析,檢測CRF在組織中的表達情況；行HE染色、甲苯胺藍改良染色以計數(shù)嗜酸性粒細胞、肥大細胞；分析CRF在各組中的的表達以及與肥大細胞、嗜酸性粒細胞的相關(guān)性。結(jié)果：嗜酸性粒細胞、肥大細胞的數(shù)量在變應(yīng)性鼻炎組(13.90±3.08,10.22±2.96)及鼻息肉組(12.59±2.69,6.23±3.23)明顯高于對照組(7.33±4.18,4.48±2.38),差異具有統(tǒng)計學(xué)意義(P0.05)；CRF的光密度值在變應(yīng)性鼻炎組(0.273±0.028)及鼻息肉組(0.264±0.019)中明顯高于正常對照組(0.229±0.015),差異具有統(tǒng)計學(xué)意義(P0.01),然而CRF在變應(yīng)性鼻炎組和變應(yīng)性鼻炎合并鼻息肉組兩組間差異無顯著性；CRF在鼻部的表達與肥大細胞、嗜酸性粒細胞的數(shù)量均呈正相關(guān)(r分別為0.732,0.582)。結(jié)論：(1).CRF在鼻部粘膜表達,可能參與了變應(yīng)性鼻炎與鼻息肉的鼻黏膜慢性炎癥反應(yīng)過程；(2).肥大細胞、嗜酸性粒細胞可能是鼻部CRF的主要來源；(3).CRF在上氣道炎癥性疾病中的表達及其與肥大細胞、嗜酸性粒細胞之間的聯(lián)系,為今后尋找新的治療方法提供了理論思路。
[Abstract]:Objective: airway inflammation is a chronic immune disease involving many inflammatory cells and inflammatory mediators. At present, the treatment of airway inflammation is not ideal. CRF plays an important role in peripheral inflammation. The purpose of this study was to investigate the expression of CRF in nasal tissues of patients with upper airway inflammation and its association with eosinophils and mast cells. To investigate the role of CRF in airway inflammatory diseases. Methods: the specimens were collected from 10 cases of allergic rhinitis complicated with nasal polyps, 9 cases of allergic rhinitis group and 7 cases of normal control group. Specimens of inferior turbinate were collected from allergic rhinitis group and normal control group. Immunohistochemical staining and image analysis were performed to detect the expression of CRF in tissues, HE staining and toluidine blue modified staining were performed to count eosinophils and mast cells. The expression of CRF in each group and its correlation with mast cells and eosinophils were analyzed. Results: the number of eosinophilic granulocytes and mast cells in allergic rhinitis group (13.90 鹵3.08 鹵10.22 鹵2.96) and nasal polyp group (12.59 鹵2.69 鹵6.23 鹵3.23) was significantly higher than that in control group (7.33 鹵4.184.48 鹵2.38). The difference was statistically significant (P0.05). The optical density of CRF in allergic rhinitis group (0.273 鹵0.028) and nasal polyp group (0.264 鹵0.019) was significantly higher than that in normal control group (0.229 鹵0.015). However, there was no significant difference in CRF between allergic rhinitis group and allergic rhinitis with nasal polyp group. The expression of CRF in the nose was positively correlated with the number of mast cells and eosinophils (r = 0.732 鹵0.582, respectively). Conclusion: (1) the expression of). CRF in nasal mucosa may be involved in the process of chronic inflammation of nasal mucosa in allergic rhinitis and nasal polyps; (2). Mast cells and eosinophilic granulocytes may be the main sources of nasal CRF. (3) the expression of). CRF in inflammatory diseases of upper airway and its relationship with mast cells and eosinophils, which provides a theoretical idea for finding new treatment methods in the future.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R765

【參考文獻】

相關(guān)期刊論文 前3條

1 王秋萍;季俊峰;江滿杰;李澤卿;;548例慢性鼻竇炎和鼻息肉患者下呼吸道相關(guān)疾病資料分析[J];臨床耳鼻咽喉科雜志;2006年20期

2 肖淑華;劉陽陽;魏連海;郭義;;肥大細胞的研究進展[J];生理科學(xué)進展;2011年02期

3 鮮墨;李靖;;合并鼻炎 鼻竇炎和鼻息肉的支氣管哮喘[J];中國實用內(nèi)科雜志;2009年04期

相關(guān)博士學(xué)位論文 前1條

1 武玉清;CRH & Urocortin與外周炎癥性疾病相關(guān)性的研究[D];南京醫(yī)科大學(xué);2007年

，

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