發(fā)布時(shí)間：2018-10-25 11:01

【摘要】：目的:探討維生素D受體(VDR)及其基因多態(tài)性與鼻咽癌的關(guān)聯(lián)。方法:采用熒光定量PCR檢測(cè)48例鼻咽癌患者(鼻咽癌組)和48例對(duì)照者(對(duì)照組)外周血單個(gè)核細(xì)胞中的VDR mRNA。運(yùn)用TaqMan-MGB熒光探針PCR法檢測(cè)所有研究對(duì)象的FokI和ApaI位點(diǎn)基因型。結(jié)果:鼻咽癌組VDR mRNA△Ct值為9.32±0.91,對(duì)照組為7.31±1.04,鼻咽癌組VDR mRNA表達(dá)量顯著低于對(duì)照組(t=10.08,P0.01)。采用多因素Logistic回歸分析,結(jié)果顯示FokI點(diǎn)Ff基因型在鼻咽癌組中的分布顯著高于對(duì)照組(調(diào)整OR=1.97,95%CI=1.33~2.91)。未發(fā)現(xiàn)ApaI位點(diǎn)各基因型在對(duì)照組和鼻咽癌組中分布存在差異。進(jìn)一步采用單倍型分析,結(jié)果顯示,與fa單倍型相比,Fa單倍型在對(duì)照組中的分布顯著高于鼻咽癌組(調(diào)整OR=0.65,95%CI=0.48~0.87)。2組FokI位點(diǎn)不同基因型中VDR mRNA表達(dá)水平差異有統(tǒng)計(jì)學(xué)意義(F=194.5,P0.01)。結(jié)論:VDR基因遺傳變異與鼻咽癌有密切關(guān)聯(lián)。
[Abstract]:Objective: to investigate the association of vitamin D receptor (VDR) and its gene polymorphism with nasopharyngeal carcinoma. Methods: VDR mRNA. in peripheral blood mononuclear cells of 48 patients with nasopharyngeal carcinoma (NPC group) and 48 controls (control group) was detected by fluorescence quantitative PCR. The genotypes of FokI and ApaI loci in all subjects were detected by TaqMan-MGB fluorescence probe PCR method. Results: VDR mRNA Ct was 9.32 鹵0.91in nasopharyngeal carcinoma group and 7.31 鹵1.04in control group. The expression of VDR mRNA in nasopharyngeal carcinoma group was significantly lower than that in control group (t = 10.08, P0.01). Multivariate Logistic regression analysis showed that the distribution of FokI point Ff genotype in nasopharyngeal carcinoma was significantly higher than that in control group (adjusted OR=1.97,95%CI=1.33~2.91). There was no significant difference in the distribution of ApaI locus genotypes between control group and nasopharyngeal carcinoma group. The results of haplotype analysis showed that the distribution of Fa haplotype in control group was significantly higher than that in nasopharyngeal carcinoma group (adjusted OR=0.65,95%CI=0.48~0.87). There were significant differences in VDR mRNA expression in different genotypes of FokI locus between two groups (FF194.5, P0.01). Conclusion: the genetic variation of VDR gene is closely related to nasopharyngeal carcinoma.
【作者單位】： 揚(yáng)州大學(xué)附屬醫(yī)院腫瘤科;寧波市第一人民醫(yī)院耳鼻咽喉科;揚(yáng)州大學(xué)附屬醫(yī)院耳鼻咽喉科;
【分類號(hào)】：R739.63

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