【摘要】：目的探討緩激肽對(duì)體外培養(yǎng)大鼠血視網(wǎng)膜屏障通透性的影響。方法將緩激肽作用于Transwell小室建立體外培養(yǎng)的大鼠視網(wǎng)膜血管內(nèi)皮細(xì)胞(REVC)間緊密連接屏障模型,通過跨上皮細(xì)胞電阻(TER)值觀察緩激肽對(duì)大鼠REVC間緊密連接結(jié)構(gòu)的影響;RT-PCR法檢測緊密連接相關(guān)蛋白ZO-1和occludin mRNA表達(dá)水平的變化。結(jié)果實(shí)驗(yàn)組作用2 h的TER值小于測量前、作用4 h組和對(duì)照組(P0.01);實(shí)驗(yàn)組作用4 h組和測量前、對(duì)照組TER值無明顯變化(P0.05)。結(jié)論緩激肽可在轉(zhuǎn)錄水平上減少緊密連接相關(guān)蛋白ZO-1和occludin mRNA表達(dá),能夠開放大鼠REVC間的緊密連接,增加血視網(wǎng)膜屏障的通透性。
[Abstract]:Objective to investigate the effect of bradykinin on the permeability of blood-retinal barrier in vitro. Methods bradykinin was applied to Transwell chamber to establish a rat model of tight junction barrier between (REVC) and vascular endothelial cells (RECs) cultured in vitro. The effect of bradykinin on the tight junction structure of rat REVC was observed by (TER) value of transepithelial cells. The expression levels of ZO-1 and occludin mRNA were detected by RT-PCR. Results the TER value of the experimental group at 2 h was lower than that of the control group (P0.01), and the TER value of the experimental group was not changed significantly (P0.05). Conclusion bradykinin can reduce the expression of ZO-1 and occludin mRNA at the transcriptional level, open the tight connection between REVC and increase the permeability of blood-retinal barrier.
【作者單位】： 牡丹江醫(yī)學(xué)院組胚教研室;
【基金】：黑龍江省自然科學(xué)基金資助項(xiàng)目(No.H2015078)
【分類號(hào)】：R774.1
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本文編號(hào)：2186636