本文選題：血管內皮生長因子 + 對氧磷酶　； 參考：《重慶醫(yī)學》2017年30期

【摘要】：目的探討血管內皮生長因子(VEGF)、對氧磷酶1(PON1)基因多態(tài)性與糖尿病視網膜病變(DR)的相關性。方法選取2013年1月至2015年12月西寧市第二人民醫(yī)院收治的糖尿病(DM)患者168例,將其分為DM組(48例)、增殖性DR(PDR)組(66例)和非增殖性DR(NPDR)組(54例);另選取同期體檢健康者104例作為對照組(NC組)。通過聚合酶鏈反應-限制性片段長度多態(tài)性(PCR-RFLP)測定血管內皮生長因子(VEGF)和對氧磷酶1(PON1)基因多態(tài)性,采用紫外分光光度計檢測所有受試者超氧化歧化酶(SOD)和丙二醛(MDA)水平。結果與NC組比較,DM組、NPDR組及PDR組收縮壓、糖化血紅蛋白水平明顯升高,差異均有統(tǒng)計學意義(P0.05);而舒張壓、膽固醇、三酰甘油、高密度脂蛋白水平比較,差異均無統(tǒng)計學意義(P0.05)。PDR組和NPDR組收縮壓比較,差異有統(tǒng)計學意義(P0.05),而舒張壓、膽固醇、三酰甘油、高密度脂蛋白、糖化血紅蛋白水平比較,差異均無統(tǒng)計學意義(P0.05)。CC、CT、TT基因型患者DR發(fā)生率分別為71.05%、56.27%、38.64%,CC基因型患者DR發(fā)生率明顯高于CT和TT基因型患者,差異有統(tǒng)計學意義(P0.05)。PON1 192位點不同基因型健康體檢者SOD、MDA水平比較,差異均無統(tǒng)計學意義(P0.05),QQ基因型DR患者體內SOD水平低于QR、RR基因型患者,MDA水平高于QR、RR基因型患者,差異均有統(tǒng)計學意義(P0.05)。結論 VEGF、PON1基因的表達影響DR的發(fā)生和進展,PON1基因可能通過影響DR患者體內氧化應激酶的表達控制疾病的發(fā)展。
[Abstract]:Objective to investigate the association between the polymorphism of vascular endothelial growth factor (VEGF) VEGFN, paraoxonase 1 (PON1) gene and diabetic retinopathy (DRN). Methods 168 patients with DM were selected from January 2013 to December 2015 in Xining second people's Hospital. They were divided into DM group (n = 48), proliferative DRN group (n = 66) and non-proliferative DRN DRN group (n = 54), and 104 healthy subjects as control group. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of vascular endothelial growth factor (VEGF) and paraoxonase 1 (PON1) gene, and the levels of superoxide dismutase (Sod) and malondialdehyde (MDAs) were detected by ultraviolet spectrophotometer. Results compared with NC group, systolic blood pressure (SBP) and glycosylated hemoglobin (HbA1c) were significantly increased in DM group (NPDR group) and PDR group (P 0.05), while diastolic blood pressure (DBP), cholesterol (TC), triglyceride (TG) and high density lipoprotein (HDL) levels were compared. There was no significant difference in systolic blood pressure (SBP) between PDR group and NPDR group, but diastolic blood pressure, cholesterol, triacylglycerol, high density lipoprotein and glycosylated hemoglobin were compared. The incidence of Dr in patients with CC genotype was significantly higher than that in patients with CT and TT genotypes, and there was a significant difference in the levels of MDA between patients with CC genotype and those with genotype CT and TT genotypes. There was significant difference in the levels of MDA in patients with different genotypic health controls at P0.05N. PON1 192 locus. The incidence of Dr in patients with CC genotype was 71.05 and 56.27, respectively, which was significantly higher than that in patients with CT and TT genotypes. The level of SOD in patients with QQ genotype Dr was lower than that in patients with QRNRR genotype, and the level of MDA in patients with QRNRR genotype was higher than that in patients with QRNRR genotype, and the difference was statistically significant (P 0.05). Conclusion the expression of VEGFG PON1 gene may affect the pathogenesis and progression of Dr. The expression of PON1 gene may control the development of disease by affecting the expression of ERK in Dr patients.
【作者單位】： 西寧市第二人民醫(yī)院眼科;青海大學醫(yī)學院藏醫(yī)學院藏醫(yī)系;
【基金】：國家自然科學基金資助項目(81560806)
【分類號】：R587.2;R774.1

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本文編號：2037094