本文選題：鼻息肉　切入點：MIF　出處：《貴州醫(yī)科大學》2017年碩士論文

【摘要】：目的:通過檢測鼻息肉組織中巨噬細胞移動抑制因子(MIF)、白細胞介素-5(IL-5)的表達和嗜酸性粒細胞(Eos)的浸潤情況,并采用視覺模擬量表(VAS)和Lund-Mackay評分法評估鼻息肉患者病情嚴重程度,分析MIF、IL-5表達與Eos浸潤程度間的相關(guān)性及兩因子表達與疾病嚴重程度間的關(guān)系,探討MIF和IL-5在鼻息肉發(fā)生過程中的作用,并分析其可能作用途徑。方法:采用VAS評分和Lund-Mackay評分評估鼻息肉患者病情嚴重程度;應(yīng)用HE染色(蘇木精-伊紅染色法)檢測鼻息肉組織、正常中鼻甲粘膜組織中EOS的浸潤程度并計數(shù);采用免疫組織化學法(SP法)檢測鼻息肉組織、正常中鼻甲組織中MIF、IL-5的表達并進行計數(shù),分析二者與疾病嚴重程度及EOS浸潤程度的相關(guān)性。結(jié)果:1.EOS在鼻息肉組織中的浸潤程度(39.30±4.49)個/HP明顯高于在對照組中的浸潤數(shù)量(7.70±3.19)個/HP,p0.05;2.MIF在鼻息肉組織中的表達(0.3693±0.0535)明顯高于對照組中的表達量(0.1557±0.0395),p0.05;3.IL-5在鼻息肉中的表達量(0.7378±0.2262)明顯高于在對照組中的表達量(0.2253±0.1061),p0.05;4.MIF、IL-5表達與Eos浸潤程度間呈正向相關(guān)性(r分別為0.793、0.843,p均0.05);5.鼻息肉患者VAS評分與MIF、IL-5表達量間呈正相關(guān)性(r分別為0.598、0.769,p均0.05),鼻竇CT Lund-Mackay評分與二者表達同樣呈正相關(guān)性(r分別為0.613、0.626,p均0.05)。結(jié)論:1.MIF、IL-5在鼻息肉組織中的高表達,說明二者共同參與了鼻息肉的發(fā)生發(fā)展;2.MIF、IL-5表達與EOS浸潤程度間呈正相關(guān),說明二者可能通過增加EOS浸潤程度促使鼻息肉的形成;3.MIF、IL-5表達與患者病情嚴重程度間呈正相關(guān),表明二者的表達可在一定程度上評估患者疾病的嚴重程度。
[Abstract]:Objective: to detect the expression of macrophage migration inhibitory factor (MIFN) and interleukin-5 (IL-5) and the infiltration of eosinophil in nasal polyps, and to evaluate the severity of nasal polyps by visual analogue scale (VAS) and Lund-Mackay score. To analyze the relationship between the expression of IL-5 and the degree of Eos invasion, and the relationship between the expression of two factors and the severity of the disease, and to explore the role of MIF and IL-5 in the pathogenesis of nasal polyps. Methods: VAS and Lund-Mackay scores were used to evaluate the severity of nasal polyps, and HE staining (hematoxylin eosin staining) was used to detect nasal polyps. The infiltration degree and count of EOS in normal mucosa of middle turbinate, the expression of MIFIL-5 in nasal polyp tissue and normal middle turbinate tissue were detected and counted by immunohistochemical method. Results 1. The infiltration degree of EOS in nasal polyps was 39.30 鹵4.49) / HP was significantly higher than that in control group (7.70 鹵3.19) and the expression of EOS in nasal polyps was 0.3693 鹵0.0535). The expression of IL-5 in nasal polyps was 0.7378 鹵0.2262), which was significantly higher than that in control group (0.2253 鹵0.1061). The positive correlation between the expression of IL-5 and the degree of Eos infiltration was 0.7930.843 and 0.05 respectively. The positive correlation between the VAS score and the expression of IL-5 in nasal polyps was 0.05% and 0.2253 鹵0.1061 respectively, and the positive correlation between the expression of IL-5 and the degree of Eos infiltration was 0.7930.843, P = 0.05, respectively. The positive correlation between the expression of IL-5 in nasal polyps and the expression of IL-5 in nasal polyps was significantly higher than that in the control group (0.2253 鹵0.1061). There was a positive correlation between 0. 5988 and 0. 769 (p = 0. 05), and there was a positive correlation between CT Lund-Mackay score of paranasal sinus and the expression of both. Conclusion: 1. MIF- IL-5 is highly expressed in nasal polyps, and the positive correlation is 0. 613 ~ 0. 626p, P = 0. 05 respectively. 2. There was a positive correlation between the expression of IL-5 in nasal polyps and the degree of EOS infiltration. It was suggested that the expression of IL-5 in nasal polyps was positively correlated with the severity of the disease by increasing the degree of EOS infiltration. These results suggest that the expression of both can be used to evaluate the severity of the disease to some extent.
【學位授予單位】：貴州醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R765.25

【參考文獻】

相關(guān)期刊論文 前10條

1 白銀;奧彥云;徐叢;邱昕;張悅;王洪田;;慢性鼻-鼻竇炎臨床表型及炎性特征的初步分析[J];臨床耳鼻咽喉頭頸外科雜志;2017年02期

2 宋鍇;龔正鵬;;糖皮質(zhì)激素對鼻息肉組織中巨噬細胞移動抑制因子水平的影響[J];貴州醫(yī)科大學學報;2016年08期

3 李春苗;馬輝娟;巴云鵬;;MIF和NF-κB及IL-1β在鼻息肉組織中的表達及意義[J];臨床耳鼻咽喉頭頸外科雜志;2016年03期

4 杜志宏;于亞峰;;NLRP3炎性小體在嗜酸粒細胞性鼻息肉發(fā)病及復(fù)發(fā)中的作用[J];山東大學耳鼻喉眼學報;2016年01期

5 張輝;鄭成彩;馮慧偉;郭成浩;;變應(yīng)性鼻炎患者外周血EOS-CSF、IL-5水平及EOS數(shù)目對局部皮質(zhì)類固醇激素治療效果的評價[J];山東大學耳鼻喉眼學報;2015年05期

6 陳旭央;張冰;胡莉蔓;;IL-17、IL-5、IL-4、IL-33等細胞因子在哮喘發(fā)病機制中的作用[J];中國醫(yī)藥導(dǎo)報;2015年14期

7 李陽陽;趙明;文連姬;徐艷萍;湯翠華;黃芳林;何曉;孟粹達;;白細胞介素-5和白細胞介素-16在變應(yīng)性鼻炎患者血清中的表達及臨床意義[J];中國耳鼻咽喉頭頸外科;2015年03期

8 胡志紅;張海軍;李健;王晶華;;嗜酸性粒細胞和IL-5在鼻息肉中的表達及在發(fā)病中的作用[J];臨床和實驗醫(yī)學雜志;2014年19期

9 劉屹;趙勇;靳元嶸;張亞寧;鄭偉紅;;VEGF、GM-CSF在初發(fā)和復(fù)發(fā)性鼻息肉中表達的關(guān)系[J];河北醫(yī)藥;2014年09期

10 鐘誠;姜振東;張學淵;魏運軍;袁偉;李琪;;鼻息肉發(fā)生部位及其與竇口鼻道復(fù)合體關(guān)系研究[J];人民軍醫(yī);2014年03期

相關(guān)博士學位論文 前1條

1 張超;IL-18和巨噬細胞移動抑制因子（MIF）與心房顫動的關(guān)系研究[D];第四軍醫(yī)大學;2010年

相關(guān)碩士學位論文 前3條

1 李賢;巨噬細胞微粒通過激活MAPK信號通路誘導(dǎo)THP-1細胞產(chǎn)生IL-8[D];南華大學;2015年

2 宋建春;鼻息肉組織中IL-5表達與Eos計數(shù)的關(guān)聯(lián)性研究[D];山西醫(yī)科大學;2014年

3 趙勁松;巨噬細胞移動抑制因子（MIF）在人食管鱗癌組織中表達的研究[D];第三軍醫(yī)大學;2007年

，

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