本文關(guān)鍵詞： 甲狀腺相關(guān)性眼病 PPAR-γ 活動性　出處：《山西醫(yī)科大學》2017年碩士論文　論文類型：學位論文

【摘要】：目的:檢測TAO患者眼眶脂肪組織以及血清中PPAR-γ的表達水平,初步探討PPAR-γ在TAO發(fā)病過程中的作用。方法:選取TAO患者4例,健康對照者4例,用RT-PCR法檢測兩組眶脂肪PPAR-γmRNA的表達水平以及用Western blot法檢測兩組眶脂肪PPAR-γ蛋白的表達水平。選取TAO患者33例(男14例,女19例),按臨床活動性評分(CAS)及99Tcm-DTPA眼眶顯像將TAO患者分為兩大組:活動期(CAS≥3,99Tcm-DTPA眼眶顯像陽性)18例(男7例,女11例)和非活動期(CAS3,99Tcm-DTPA眼眶顯像陰性)15例(男7例,女8例)。單純Graves病(GD)患者16例(男6例,女10例),健康對照者(CON)15例(男7例,女8例)。用Elisa法檢測四組血清PPAR-γ表達水平。采用SPSS19.0進行統(tǒng)計分析,計量資料采用x±s表示,計數(shù)資料采用例數(shù)(構(gòu)成比)表示,正態(tài)分布數(shù)據(jù)均數(shù)的比較采用方差分析,非正態(tài)分布數(shù)據(jù)均數(shù)的比較采用秩和檢驗,構(gòu)成比的比較采用卡方檢驗,檢驗水準α=0.05。結(jié)果:1.兩組患者眶脂肪中PPAR-γmRNA的表達水平:TAO組2.640±0.525,CON組1.182±0.272,TAO組明顯高于CON組,差異有統(tǒng)計學意義(P0.05)。2.眶脂肪中兩組患者PPAR-γ蛋白的表達水平:TAO組0.992±0.104,CON組0.634±0.0291,T AO組明顯高于CON組,差異有統(tǒng)計學意義(P0.05)。3.血清中四組患者PPAR-γ的表達水平:活動組0.0133±0.0031pg/ml,非活動組0.00903±0.00421bpg/ml,GD組0.0190±0.00323pg/ml,CON組0.00813±0.00774 pg/ml。四組比較,差異有統(tǒng)計學意義(F=16.199,P0.001),其中活動組與非活動組比較,活動組明顯高于非活動組,差異有統(tǒng)計學意義(P=0.0150.05);活動組與GD組比較,活動組明顯低于GD組,差異有統(tǒng)計學意義(P=0.001);活動組與CON組比較,活動組明顯高于CON組,差異有統(tǒng)計學意義(P=0.0030.05);非活動組與GD組比較,非活動組明顯低于G D組,差異有統(tǒng)計學意義(P0.001);非活動組與CON組比較,差異無統(tǒng)計學意義(P=0.6100.05);GD組與CON組比較,GD組明顯高于CON組,差異有統(tǒng)計學意義(P0.001)。男女患者血清中PPAR-γ表達水平無統(tǒng)計學差異(P0.05),不同年齡患者血清中PPAR-γ表達水平亦無統(tǒng)計學差異(P0.05)。結(jié)論:1.TAO患者眶脂肪PPAR-γ基因轉(zhuǎn)錄水平及蛋白表達水平均顯著增加。2.活動期TAO患者血清中PPAR-γ表達水平顯著增高。3.甲狀腺功能亢進患者血清中PPAR-γ表達水平顯著增高。4.年齡和性別對TAO患者血清中PPAR-γ的表達無顯著影響。
[Abstract]:Objective: to detect the expression of PPAR- 緯 in orbital adipose tissue and serum of TAO patients, and to explore the role of PPAR- 緯 in the pathogenesis of TAO. The expression of PPAR- 緯 mRNA in orbital fat and the expression of PPAR- 緯 protein in orbital fat were detected by RT-PCR method and Western blot method respectively. 19 female patients were divided into two groups according to the clinical activity score (CAS) and 99Tcm-DTPA orbital imaging: 18 cases (male 7 cases, female 11 cases) with CAS399Tcm-DTPA orbital imaging positive and 15 cases (male 7 cases) with CAS399Tcm-DTPA negative orbital imaging (male 7 cases), with CAS399Tcm-DTPA imaging negative (7 cases), the patients with CAS399Tcm-DTPA orbital imaging were divided into two groups: CAS399Tcm-DTPA imaging was positive in 18 cases (male 7 cases, female 11 cases). There were 16 patients (6 males, 10 females) with Graves disease and 15 healthy controls (7 males and 8 females). The expression of PPAR- 緯 in serum of the four groups was detected by Elisa method. SPSS19.0 was used for statistical analysis, and the measured data were expressed as x 鹵s. The counting data are expressed by examples (composition ratio), the mean of normal distribution data is compared by ANOVA, the non-normal distribution data mean is compared by rank sum test, and the composition ratio is compared by chi-square test. Results the expression level of PPAR- 緯 mRNA in the orbital fat of the two groups was significantly higher than that in the CON group (2.640 鹵0.525) and 1.182 鹵0.272 respectively. The expression level of PPAR- 緯 protein in the two groups was significantly higher than that in the CON group (0.634 鹵0.0291T AO, 0.992 鹵0.104CON, 0.634 鹵0.0291T). The expression level of PPAR- 緯 in the four groups was 0.0133 鹵0.0031 PG / ml in the active group, 0.00903 鹵0.00421 bpg / ml in the inactive group, 0.0190 鹵0.00323pg / ml / ml group in the GD group, 0.00813 鹵0.00774 pg / ml / ml in the four groups. There was significant difference in the expression of PPAR- 緯 between the active group and the inactive group, and the difference between the active group and the inactive group was significantly higher than that in the inactive group, and the expression of PPAR- 緯 in the active group was significantly higher than that in the inactive group. The difference between active group and GD group was significantly lower than that in GD group, the difference was statistically significant, and the difference between active group and CON group was significantly higher than that in CON group (P 0.0030.05), the difference between active group and GD group was statistically significant, and the difference between inactive group and GD group was significantly higher than that in active group and GD group. There was no significant difference between the inactive group and the CON group, and the difference between the GD group and the CON group was higher than that in the CON group. There was no significant difference in the expression of PPAR- 緯 between male and female patients and there was no significant difference in the expression of PPAR- 緯 between male and female patients with different ages. Conclusion the transcription level and protein expression of PPAR- 緯 gene in orbital fat of patients with TAO are not significantly different from those of patients of different ages. The expression of PPAR- 緯 in serum of patients with hyperthyroidism was significantly higher than that of patients with hyperthyroidism. 4. Age and sex had no significant effect on the expression of PPAR- 緯 in serum of patients with TAO.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R581;R771.3

【參考文獻】

相關(guān)期刊論文 前2條

1 劉紅霞;吳中耀;;甲狀腺相關(guān)眼病患者血清中抵抗素水平的測定[J];眼科研究;2010年06期

2 鮑縵夕;鄒大進;魯謹;;不同甲狀腺功能狀態(tài)下人體血清脂聯(lián)素水平的變化[J];中國臨床康復;2006年36期

，

本文編號：1497630