本文關鍵詞：噪聲暴露后大鼠聽皮層Parvalbumin表達水平的變化　出處：《安徽醫(yī)科大學》2015年碩士論文　論文類型：學位論文

  更多相關文章： 噪聲暴露 聽閾 聽皮層 小清蛋白 γ-氨基丁酸

【摘要】：目的觀察噪聲暴露對大鼠聽覺腦性誘發(fā)反應(Auditory brainstem response,ABR)的影響以及暴露后聽皮層中鈣結合蛋白中小清蛋白Parvalbumin(PV)表達水平的變化,研究噪聲暴露對大鼠聽皮層中PV表達量的影響,探討噪聲引起的聽力損失、耳鳴及其他聽覺異常等疾病的可能機制。方法將31只雄性SD大鼠隨機分為實驗組16只以及對照組15只。將實驗組大鼠左耳暴露于帶寬為一個倍頻程,中心頻率為16 k Hz,聲音強度為116d B聲強級的噪聲中,暴露時間為1小時。分別于暴露前及暴露后第七天測定所有大鼠的雙耳聽性腦干誘發(fā)反應,評估其聽力損失程度,應用免疫組化及蛋白質印記(Western Blot)方法檢測噪聲暴露后第七天大鼠聽皮層中小清蛋白PV的變化,推測噪聲暴露后大鼠聽皮層中PV變化可能的原因及機制。結果(1)聽力變化:暴露前實驗組大鼠左右耳聽閾均為20.00±5.0d B SPL,暴露后第七天左耳的聽閾為75.0±5.0d B SPL,右耳聽閾為18.3±2.9d B SPL,相較于暴露前,實驗組大鼠左耳聽閾明顯提高。暴露后左右耳比較,左耳聽閾明顯較右耳高,差異具有統(tǒng)計學意義(P0.05);右耳聽閾暴露前后無明顯改變,差異無統(tǒng)計學意義(P0.05)。實驗組右耳同對照組比較無明顯改變,差異無統(tǒng)計學意義(P0.05)。(2)聽皮層中PV表達水平的變化:噪聲暴露后第七天,免疫組化及Western Blot檢測結果均顯示實驗組大鼠左側聽皮層中PV的表達量較右側高,同時實驗組大鼠左側大腦皮層中PV的表達量較對照組大鼠表達量高,差異均具有統(tǒng)計學意義(P0.05);實驗組大鼠右側聽皮層中PV表達量同對照組比較,差異具有統(tǒng)計學意義(P0.05)。結論單耳噪聲暴露后第7天,大鼠的暴露耳聽閾明顯提高,同時,在噪聲暴露后,免疫組化及Western Blot結果均顯示實驗組大鼠聽皮層PV升高,左側表達量較右側升高明顯,這可能中樞神經系統(tǒng)抑制性降低,與對聲音的過度刺激的一種保護作用、聲音刺激后細胞代謝增強及興奮性信號輸入的增多有關,聽皮層PV的變化可能與耳鳴及其它聽覺功能改變有關。
[Abstract]:Objective to observe the auditory brain evoked response to noise exposure in rats. The effect of ABR and the changes of parvalbumin PVs expression level in auditory cortex after exposure. To study the effect of noise exposure on the expression of PV in the auditory cortex of rats and to explore the hearing loss caused by noise. Methods 31 male SD rats were randomly divided into experimental group (n = 16) and control group (n = 15). The center frequency is 16 kHz and the sound intensity is 116 dB. The exposure time was 1 hour. The auditory brainstem responses of all rats were measured before and 7th days after exposure to assess the degree of hearing loss. The changes of small and medium albumin PV in auditory cortex of rats 7th days after noise exposure were detected by immunohistochemistry and Western blot method. The possible causes and mechanism of PV changes in the auditory cortex of rats after noise exposure were speculated. Results 1) hearing changes: the hearing thresholds of the left and right ears of the experimental group were 20.00 鹵5.0 dB SPL before exposure. The hearing threshold of left ear and right ear was 75.0 鹵5.0d and 18.3 鹵2.9d respectively, which was higher than that before exposure. The hearing threshold of the left ear in the experimental group was significantly higher than that in the right ear after exposure, and the difference was statistically significant (P 0.05). There was no significant change in the right ear hearing threshold before and after exposure, and the difference was not statistically significant (P 0.05). There was no significant change in the right ear in the experimental group compared with the control group. There was no significant difference in the expression of PV in the auditory cortex: 7th days after noise exposure. The results of immunohistochemistry and Western Blot showed that the expression of PV in the left auditory cortex of the experimental group was higher than that in the right. At the same time, the expression of PV in the left cerebral cortex of the experimental group was higher than that of the control group, and the difference was statistically significant (P 0.05). Compared with the control group, the expression of PV in the right auditory cortex of the experimental group was significantly higher than that in the control group (P 0.05). Conclusion on the 7th day after monaural noise exposure, the hearing threshold of exposed ears in the experimental group was significantly higher than that in the control group. After noise exposure, the results of immunohistochemistry and Western Blot showed that PV in auditory cortex of experimental group was increased, and the expression of left side was significantly higher than that of right, which may decrease the inhibition of central nervous system. The changes of PV in auditory cortex may be related to tinnitus and other auditory function changes.
【學位授予單位】：安徽醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R764

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本文編號：1401375