慢性帕瑞昔布鈉處理對小鼠學(xué)習(xí)記憶、焦慮及抑郁行為的影響
[Abstract]:Objective: to investigate the role of cyclooxygenase-2 (COX-2) in learning and memory. Parracoxib sodium, an inhibitor of COX-2, can improve learning and memory impairment under pathological conditions, but its effect on learning and memory function under normal physiological condition is still unclear. In addition, the effect of paracoxib sodium on anxiety and depression behavior remains to be clarified. In this study, the effects of chronic paracoxib sodium treatment on learning and memory, anxiety and depression in mice were investigated. The effects of chronic paracoxib sodium on the expression of synaptophysin in the amygdala and hippocampus of mice were examined in order to explore the possible neuromechanism. Methods: 1. Chronic paracoxib sodium treatment: ICR mice were intraperitoneally injected with paracoxib sodium (2.5, 5.0 and 10 mg/kg) for 28 days, and control mice were injected with equal volume of saline. Novel object recognition (NOR) test: after 8 days of continuous injection of Parracoxib sodium, a novel object recognition test was performed. In the short-term NOR test, the time interval between the training phase and the detection phase is 4 hours, and in the long-term NOR test, the time interval between the training phase and the detection phase is 24 hours. The total inquiring time of the two bodies in the training phase and the discrimination index in the test phase were recorded and analyzed. 3. Y maze test: after 11 days of continuous injection of Parracoxib sodium, the Y labyrinth test was performed. The total number of entrances and the ratio of arm alternation in 8 minutes were recorded in the mice. 4. Elevated cross labyrinth test: after 17 days of continuous injection of Parracoxib sodium, the mice were recorded and analyzed the number of closed arms in 5 minutes, the percentage of open arm entry times and the percentage of open arm stay time. 5. Tail suspension test: after 18 days of continuous injection of Parracoxib sodium, the tail suspension test was performed to record the immobility time of mice within 5 minutes. 6. Forced swimming test: after 19 days of continuous injection of Parracoxib sodium, forced swimming test was performed to record the immobility time of the mice within 5 minutes. 7. Western blot test: after the behavioral test was completed, the head was decapitated and the brain was removed. The expression of synaptophysin in hippocampus and amygdaloid nucleus was detected by. Western blot isolation from hippocampus and amygdala. Results: 1. In the short-term and long-term NOR tests, there was no significant difference in the total inquiry time of the two objects between the four groups in the training stage; in the detection stage, the discrimination index of the 10-mg/kg Parracoxib sodium group was significantly higher than that of the physiological saline group. 2. In the Y labyrinth test, there was no significant difference in the total frequency of the arm between the four groups, and the ratio of the arm alternation in the 10 mg/kg paracoxib sodium group was significantly higher than that in the physiological saline group. 3. In the elevated maze test, there was no significant difference in the number of closed arms between the four groups, 5 and 10 mg/kg paracoxib sodium group were significantly higher than the physiological saline group in the percentage of open arm entry times and the percentage of open arm residence time. 4. The results showed that there was no significant difference between the four groups in the number of open arm entrances and the percentage of open arm residence time. In the tail suspension test, there was no significant difference in immobility time among the four groups. 5. In the forced swimming test, there was no significant difference in the immobility time between the four groups. 6. The expression level of synaptophysin in hippocampus and amygdala in 10mg/kg group was significantly higher than that in saline group. Conclusions: 1. Chronic paracoxib sodium treatment can enhance the learning and memory function of mice, and its mechanism may be related to the up-regulation of synaptophysin expression in hippocampus and amygdala by Parracoxib sodium. 2. Chronic paracoxib sodium treatment could improve the anxiety behavior of mice, but had no effect on the depression behavior of mice.
【學(xué)位授予單位】:南華大學(xué)
【學(xué)位級別】:碩士
【學(xué)位授予年份】:2015
【分類號】:R614
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