缺氧誘導(dǎo)因子-1α在腦缺血中的作用及其機(jī)制研究
發(fā)布時(shí)間：2019-03-08 15:19
【摘要】：目的研究在腦缺血過(guò)程中HIF-1α、VEGF因子的表達(dá)及其作用機(jī)制。方法選取60只成年健康雄性SD大鼠,體重250~300g,清潔級(jí)。將大鼠隨機(jī)分為兩組:實(shí)驗(yàn)組和對(duì)照組,每組各30只。造模取得成功2h后,實(shí)驗(yàn)組注射HIF-1α質(zhì)粒液,對(duì)照組注射同等量的PBS緩沖液,按照注射后時(shí)間不同分為6h,24h,48h,72h,7d五個(gè)時(shí)間點(diǎn),每個(gè)時(shí)間點(diǎn)亞組為6只大鼠。采用TTC染色法觀察各組大鼠腦梗塞面積,來(lái)判斷腦組織缺血程度;采用免疫印跡試驗(yàn)檢測(cè)腦組織梗塞區(qū)中HIF-1α和VEGF的蛋白表達(dá)水平。結(jié)果1 TTC染色法觀察各組大鼠腦梗塞面積與對(duì)照組相比,實(shí)驗(yàn)組6h、24h、48h時(shí)間點(diǎn)上梗塞面積無(wú)明顯差異(P0.05),而在72h、7d時(shí)間點(diǎn)上有差異,其中7d時(shí)差異更為顯著(P0.01)。2注射HIF-1α質(zhì)粒液對(duì)大鼠死亡率的影響大鼠在缺血再灌注后注射HIF-1α質(zhì)粒液,在4h~60h之間死亡率最高,若存活時(shí)間超過(guò)72h死亡率低(P0.01)。3免疫印跡試驗(yàn)檢測(cè)腦組織梗死區(qū)中HIF-1α和VEGF兩種因子其蛋白的表達(dá)結(jié)果對(duì)照組和實(shí)驗(yàn)組中兩種蛋白表達(dá)量在6h、24h逐漸增高(P0.01),24h時(shí)表達(dá)量最高,48h、72h、7d時(shí)逐漸下降,7d時(shí)表達(dá)量最低(P0.01)。與對(duì)照組相比,實(shí)驗(yàn)組各個(gè)時(shí)間點(diǎn)HIF-1α和VEGF蛋白表達(dá)量均升高,具有統(tǒng)計(jì)學(xué)意義(P0.05)。對(duì)HIF-1α、VEGF兩種蛋白表達(dá)水平進(jìn)行相關(guān)性分析,結(jié)果表明VEGF和HIF-1α的表達(dá)具有正相關(guān)性。結(jié)論1 HIF-1α質(zhì)粒在大鼠腦缺血再灌注損傷中,有助于改善腦組織缺血梗死區(qū),使其縮小。2在腦缺血再灌注損傷中,HIF-1α、VEGF因子在此調(diào)控過(guò)程中起著非常重要的作用。
[Abstract]:Objective to study the expression and mechanism of HIF-1 偽 and VEGF during cerebral ischemia. Methods 60 adult healthy male SD rats weighing 250 g and 300 g were selected. Rats were randomly divided into two groups: experimental group and control group, 30 rats in each group. Two hours after the establishment of the model, the experimental group was injected with HIF-1 偽 granule solution and the control group was injected with the same amount of PBS buffer. According to the different time after injection, the rats were divided into 6 hours, 24 hours, 48 hours, 72 hours. At 7 days, 6 rats in each subgroup were divided into five time points. The cerebral infarction area of each group was observed by TTC staining to determine the degree of cerebral ischemia, and the protein expression of HIF-1 偽 and VEGF in cerebral infarction area was detected by immunoblotting test. Results 1Compared with the control group, there was no significant difference in the infarct area between the experimental group and the control group at 6 h, 24 h, 48 h (P0.05), but at 72 h and 7 d, there was no significant difference in the infarct area between the experimental group and the control group by TTC staining. The effect of injection of HIF-1 偽 granule solution on the mortality of rats was more significant at the 7th day (P0.01). (2) the death rate of rats injected with HIF-1 偽 granule solution after ischemia reperfusion was the highest among 4h~60h. If the survival time was longer than 72 hours, the mortality was low (P0.01). 3 the expression of HIF-1 偽 and VEGF in cerebral infarction area was detected by immunoblotting assay. The expression of two proteins in the control group and the experimental group was 6 hours. The expression level increased gradually at 24 h (P0.01), the highest at 24 h, the highest at 48 h, at 72 h, gradually decreased at 7 d, and the lowest at 7 d (P 0.01). Compared with the control group, the expression of HIF-1 偽 and VEGF protein in the experimental group was significantly higher than that in the control group (P0.05). The expression levels of HIF-1 偽 and VEGF were analyzed. The results showed that there was a positive correlation between the expression of VEGF 偽 and HIF-1 偽. Conclusion (1) HIF-1 偽 plasmid can improve the ischemic infarct area and reduce it in cerebral ischemia reperfusion injury in rats. 2 in cerebral ischemia reperfusion injury, HIF-1 偽 and VEGF factor play a very important role in the regulation of cerebral ischemia reperfusion injury. [WT5HZ] conclusion\?
【學(xué)位授予單位】：華北理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：R614
本文編號(hào)：2436938
[Abstract]:Objective to study the expression and mechanism of HIF-1 偽 and VEGF during cerebral ischemia. Methods 60 adult healthy male SD rats weighing 250 g and 300 g were selected. Rats were randomly divided into two groups: experimental group and control group, 30 rats in each group. Two hours after the establishment of the model, the experimental group was injected with HIF-1 偽 granule solution and the control group was injected with the same amount of PBS buffer. According to the different time after injection, the rats were divided into 6 hours, 24 hours, 48 hours, 72 hours. At 7 days, 6 rats in each subgroup were divided into five time points. The cerebral infarction area of each group was observed by TTC staining to determine the degree of cerebral ischemia, and the protein expression of HIF-1 偽 and VEGF in cerebral infarction area was detected by immunoblotting test. Results 1Compared with the control group, there was no significant difference in the infarct area between the experimental group and the control group at 6 h, 24 h, 48 h (P0.05), but at 72 h and 7 d, there was no significant difference in the infarct area between the experimental group and the control group by TTC staining. The effect of injection of HIF-1 偽 granule solution on the mortality of rats was more significant at the 7th day (P0.01). (2) the death rate of rats injected with HIF-1 偽 granule solution after ischemia reperfusion was the highest among 4h~60h. If the survival time was longer than 72 hours, the mortality was low (P0.01). 3 the expression of HIF-1 偽 and VEGF in cerebral infarction area was detected by immunoblotting assay. The expression of two proteins in the control group and the experimental group was 6 hours. The expression level increased gradually at 24 h (P0.01), the highest at 24 h, the highest at 48 h, at 72 h, gradually decreased at 7 d, and the lowest at 7 d (P 0.01). Compared with the control group, the expression of HIF-1 偽 and VEGF protein in the experimental group was significantly higher than that in the control group (P0.05). The expression levels of HIF-1 偽 and VEGF were analyzed. The results showed that there was a positive correlation between the expression of VEGF 偽 and HIF-1 偽. Conclusion (1) HIF-1 偽 plasmid can improve the ischemic infarct area and reduce it in cerebral ischemia reperfusion injury in rats. 2 in cerebral ischemia reperfusion injury, HIF-1 偽 and VEGF factor play a very important role in the regulation of cerebral ischemia reperfusion injury. [WT5HZ] conclusion\?
【學(xué)位授予單位】：華北理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：R614
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