【摘要】：隨著現(xiàn)代交通、建筑、工業(yè)的迅猛發(fā)展,高能量損傷患者數(shù)量在外傷患者中所占比例逐年增加,脊髓損傷(Spinal Cord Injury,SCI)的發(fā)生率呈逐年上升趨勢。脊髓損傷是骨科領(lǐng)域常見的疾病之一,且具有高發(fā)性,高致殘率和多損傷嚴重等特點,所帶來的后果可能是災(zāi)難性的,輕者喪失勞動能力,重者不能活動、小便失禁、喪失生活自理能力。據(jù)統(tǒng)計分析發(fā)達國家的SCI發(fā)病率為28.3～45人/百萬人/年,我國發(fā)病率6.7人/百萬人/年。損傷發(fā)生后患者的神經(jīng)功能恢復(fù)率極低,因此對于脊髓損傷的后續(xù)治療,如何提高治愈率是現(xiàn)在醫(yī)學界面臨的難題之一,也是重點研究領(lǐng)域之一。目前針對脊髓損傷已開展大量的研究,但其修復(fù)主要面臨的問題是:神經(jīng)元的凋亡,軸突再生能力差以及損傷所造成的抑制性微環(huán)境。而隨著研究的深入,尤其是細胞移植研究結(jié)果表明,細胞移植可以促進軸突的再生和髓鞘化,進而替代凋亡的神經(jīng)細胞,從而促進脊髓損傷的修復(fù),為感覺及運動功能的恢復(fù)創(chuàng)造條件。對于細胞移植治療脊髓損傷而言,如何提高細胞的活力和分化能力是移植成功的關(guān)鍵。而現(xiàn)有的研究表明,低強度脈沖超聲波(low intensity pulsed ultrasound stimulation, LIPUS)干預(yù)種子細胞能夠明顯的提高細胞的增殖和分化能力。然而怎樣選取合適的干預(yù)參數(shù)使得治療效果最佳是難題之一。對于多目標尋優(yōu),基于遺傳算法改進的BP神經(jīng)網(wǎng)絡(luò)能夠通過全局搜索,得出最優(yōu)解。本實驗首先搭建一套超聲激勵系統(tǒng),包括信號發(fā)生器、放大器、電源和檢測模塊。其中放大器模塊采用THS4062芯片搭建而成的。然后通過醫(yī)學實驗得出實驗結(jié)果。將BP神經(jīng)網(wǎng)絡(luò)與遺傳算法相結(jié)合,利用MATLAB軟件對實驗結(jié)果進行預(yù)測仿真分析,得出最佳實驗條件,即對BMSCs的增殖、活性影響最佳激勵參數(shù)的組合。且經(jīng)過驗證實驗,證明預(yù)測結(jié)果的正確性,為后期的移植實驗做準備。本文基于低強度脈沖超聲波對骨髓間充質(zhì)干細胞的生物學效應(yīng),利用不同參數(shù)組合的LIPUS刺激細胞,并通過采取基于遺傳算法改進的BP網(wǎng)絡(luò)模型進行預(yù)測。通過對神經(jīng)網(wǎng)絡(luò)計算出的實驗條件進行驗證實驗,得出此時的細胞活性值與比對照組提高了 24.9%,效果顯著,且預(yù)測的最佳條件下得到的細胞活性值優(yōu)于其他預(yù)設(shè)實驗條件下所得到的。
[Abstract]:With the rapid development of modern traffic, construction and industry, the proportion of high-energy injury patients in trauma patients increased year by year, and the incidence of spinal cord injury (Spinal Cord Injury,SCI) increased year by year. Spinal cord injury is one of the most common diseases in orthopedic field, and it has the characteristics of high incidence, high disability rate and severe injury. The result may be disastrous, the light person is incapacitated to work, the heavy person is unable to move, the urine is incontinence, and so on. Loss of ability to take care of oneself. According to statistics and analysis, the incidence of SCI in developed countries is 28.30.45 people / million people / year, and the incidence rate in China is 6.7 people / million people / year. The recovery rate of neurological function in patients with spinal cord injury is very low. Therefore, how to improve the cure rate of spinal cord injury is one of the difficult problems in the medical field and one of the key research fields. At present, a lot of researches have been carried out on spinal cord injury, but the main problems in repairing spinal cord injury are neuron apoptosis, poor axon regeneration ability and the inhibitory microenvironment caused by injury. With the development of research, especially the results of cell transplantation, cell transplantation can promote axon regeneration and myelination, and then replace apoptotic nerve cells, thus promoting the repair of spinal cord injury. To create conditions for the recovery of sensory and motor functions. For the treatment of spinal cord injury by cell transplantation, how to improve cell viability and differentiation ability is the key to successful transplantation. However, current studies have shown that low intensity pulsed ultrasound (low intensity pulsed ultrasound stimulation, LIPUS) can significantly improve the proliferation and differentiation of seed cells. However, how to select the appropriate intervention parameters to make the best treatment is one of the problems. For multi-objective optimization, the improved BP neural network based on genetic algorithm can obtain the optimal solution by global search. In this experiment, a set of ultrasonic excitation system including signal generator, amplifier, power supply and detection module is built. The amplifier module is made of THS4062 chip. Then the experimental results are obtained through medical experiments. By combining BP neural network with genetic algorithm, the experimental results are predicted and simulated by MATLAB software, and the optimal experimental conditions are obtained, that is, the combination of the best excitation parameters affecting the proliferation of BMSCs and the activity of BMSCs. The experimental results proved the correctness of the prediction, and prepared for the later transplantation experiment. Based on the biological effects of low-intensity pulsed ultrasound on bone marrow mesenchymal stem cells (BMSCs), different parameter combinations of LIPUS were used to stimulate the cells, and the improved BP neural network model based on genetic algorithm was adopted to predict the effects. According to the experimental conditions calculated by neural network, the results show that the cell activity at this time is 24. 9% higher than that of the control group, and the effect is remarkable. The predicted values of cell activity under the optimal conditions are better than those obtained under other preset experimental conditions.
【學位授予單位】：天津科技大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R651.2

【參考文獻】

相關(guān)期刊論文 前10條

1 劉劍鋒;朱平;馬守原;陳海旭;王曙霞;李珊;;姜黃素通過血紅素加氧酶-1信號通路促進脂肪源干細胞增殖和旁分泌研究[J];中國心血管雜志;2015年03期

2 李宇博;丁立祥;;脊髓損傷的近期研究進展[J];中國矯形外科雜志;2014年22期

3 趙鵬程;呂永鋼;鄒楊;張小梅;陳國寶;楊力;;電刺激聯(lián)合干細胞修復(fù)周圍神經(jīng)損傷的研究進展[J];生物化學與生物物理進展;2012年06期

4 馬永杰;云文霞;;遺傳算法研究進展[J];計算機應(yīng)用研究;2012年04期

5 曹中華;王金光;;脊髓損傷治療進展[J];海南醫(yī)學;2012年06期

6 陳紅梅;朱若寒;;遺傳算法研究現(xiàn)狀與應(yīng)用[J];科技信息;2011年18期

7 趙華;陳允震;劉海春;謝斌;陳劍;;自體骨髓基質(zhì)干細胞移植聯(lián)合電刺激治療脊髓損傷的實驗研究[J];中國矯形外科雜志;2010年12期

8 劉然;范洪學;劉世文;;物理治療因子對干細胞調(diào)控機制的影響[J];中國組織工程研究與臨床康復(fù);2010年23期

9 薛玉仙;高劍峰;劉軻;;電針干預(yù)對老齡大鼠腦缺血再灌注海馬齒狀回內(nèi)源性神經(jīng)干細胞增殖分化的影響[J];北京中醫(yī)藥大學學報(中醫(yī)臨床版);2009年05期

10 邵陽;肖波;楊曉蘇;趙敏;楊期東;;電磁場在組織工程修復(fù)脊髓損傷中應(yīng)用[J];中國醫(yī)學物理學雜志;2008年06期

相關(guān)博士學位論文 前3條

1 曲揚;聯(lián)合應(yīng)用姜黃素與神經(jīng)干細胞治療脊髓損傷的實驗研究[D];吉林大學;2014年

2 寧廣智;天津市脊髓損傷流行病學調(diào)查研究[D];天津醫(yī)科大學;2012年

3 李貴濤;骨髓基質(zhì)細胞源性神經(jīng)干細胞坐骨神經(jīng)和脊髓移植實驗[D];第一軍醫(yī)大學;2006年

相關(guān)碩士學位論文 前4條

1 井樂樂;毫瓦級標準壓電超聲換能器的研制與性能測試[D];鄭州大學;2014年

2 趙娜;基于BP神經(jīng)網(wǎng)絡(luò)的汽車動態(tài)稱重系統(tǒng)數(shù)據(jù)處理研究[D];太原理工大學;2012年

3 韓建莉;基于脈沖耦合神經(jīng)網(wǎng)絡(luò)的圖像處理[D];西安電子科技大學;2010年

4 蔣葵;醫(yī)學超聲圖像的計算機輔助分析系統(tǒng)[D];蘇州大學;2007年

，

本文編號：2244213