創(chuàng)傷性軸索損傷合并低氧血癥大鼠通氣復蘇效果的研究
發(fā)布時間：2018-09-09 17:10
【摘要】：研究目的1.在創(chuàng)傷性軸索損傷(TAI)合并低氧血癥性二次腦損傷(SBI)大鼠模型基礎上進行不同氧濃度復蘇,分析比較不同氧濃度復蘇之間大鼠腦組織勻漿氧自由基指標的變化規(guī)律,探討不同氧濃度復蘇對脂質過氧化的影響。2.在創(chuàng)傷性軸索損傷(TAI)合并低氧血癥性二次腦損傷(SBI)大鼠模型基礎上進行不同氧濃度復蘇,分析比較不同氧濃度復蘇之間大鼠腦組織病理學的變化規(guī)律,探討不同氧濃度復蘇對軸索損傷的影響。3.在創(chuàng)傷性軸索損傷(TAI)合并低氧血癥性二次腦損傷(SBI)大鼠模型基礎上進行不同氧濃度復蘇,探究此模型的適宜的通氣復蘇策略。研究方法1.采用自制聯(lián)合損傷裝置制作TAI模型,經小動物呼吸機吸入低濃度氧構建低氧血癥,從而形成TAI合并低氧血癥的大鼠模型。2.在此模型基礎上,對氧復蘇后大鼠腦組織勻漿進行檢測,研究不同氧濃度復蘇對大鼠脂質過氧化的影響。3.在此模型基礎上,對氧復蘇后大鼠腦組織NF200免疫組化染色,研究不同氧濃度復蘇對大鼠腦組織病理變化的影響。研究結果1.與Sham組相比,TAI+H組及各復蘇組24h時MDA含量明顯升高,1w后恢復至正常水平;各復蘇組與TAI+H組比較,給予21%、50%氧復蘇時MDA下降,差異有統(tǒng)計學意義(P0.05);各復蘇組隨氧濃度升高MDA值逐漸升高。TAI+H組及各復蘇組24h時SOD含量明顯下降,與Sham組比較有統(tǒng)計學意義(P0.05),1w時仍低于正常水平;各復蘇組與TAI+H組比較,給予21%氧復蘇時SOD值明顯升高(P0.05),給予50%、75%氧復蘇時SOD差異無統(tǒng)計學意義(P0.05),給予100%氧復蘇時SOD值明顯降低(P0.05);各復蘇組隨氧濃度升高SOD值逐漸降低。2.腦干部位NF200免疫組化染色顯示,24h時TAI合并缺氧組的陽性染色明顯強于各復蘇組,21%氧濃度時的陽性染色最弱;各組陽性染色隨時間而減弱,1w時各組陽性染色明顯弱于24h,此時TAI合并缺氧組的陽性染色仍明顯強于各復蘇組,21%氧濃度時的陽性染色仍最弱。染色陽性的神經軸索半定量分析顯示,24h時TAI合并缺氧組明顯高于各復蘇組,21%氧濃度時的值最低;各組數值隨時間而降低,1w時各組值明顯低于24h,此時TAI合并缺氧組的值仍高于各復蘇組,21%氧濃度的值仍為最低。研究結論1、給氧治療可以改善創(chuàng)傷后缺氧大鼠的低氧血癥,減輕脂質過氧化導致的損傷,但不同復蘇氧濃度的治療效果差異較大。2、適當濃度給氧治療可以顯著減低軸索損傷的程度,起到神經保護作用。3、對于此TAI合并缺氧動物模型的急性期短時間氧復蘇濃度不能超過50%。
[Abstract]:Objective 1. On the basis of (SBI) rat model of traumatic axonal injury (TAI) with hypoxia-induced secondary brain injury (SBI), different oxygen concentrations were resuscitated, and the changes of oxygen free radical index in brain homogenate of rats were analyzed and compared during different oxygen concentration resuscitation. To explore the effect of different oxygen concentration resuscitation on lipid peroxidation. On the basis of (SBI) rat model of traumatic axonal injury (TAI) combined with hypoxia-induced secondary brain injury (SBI), different oxygen concentration resuscitation was carried out, and the pathological changes of brain tissue were analyzed and compared between different oxygen concentration resuscitation. To investigate the effect of oxygen resuscitation on axonal damage. Different oxygen concentration resuscitation was carried out on the basis of traumatic axonal injury (TAI) combined with hypoxia-induced secondary brain injury (SBI) in rats, and the appropriate ventilation resuscitation strategy was explored. Method 1. The TAI model was made by using a self-made combined injury device. Hypoxemia was constructed by inhaling low concentration oxygen through a small animal ventilator, and then the rat model of TAI combined with hypoxemia was established. 2. On the basis of this model, the brain homogenate of rats after oxygen resuscitation was detected to study the effect of different oxygen concentration resuscitation on lipid peroxidation in rats. On the basis of this model, the effects of different oxygen concentration resuscitation on the pathological changes of rat brain tissue were studied by immunohistochemical staining of NF200 in brain tissue after oxygen resuscitation. Results 1. Compared with Sham group, MDA content in Tai H group and resuscitation group increased significantly at 24 h, and then returned to normal level at 1 week after resuscitation. Compared with TAI H group, MDA decreased during 50% oxygen resuscitation in each resuscitation group. There was significant difference between the two groups (P0.05), the SOD content in the resuscitation group gradually increased with the increase of oxygen concentration. The SOD content in the Tai H group and the resuscitation group decreased significantly at 24 hours, which was significantly lower than that in the Sham group (P0.05) at 1 week, and the SOD content in each resuscitation group was lower than that in the TAI H group at 1 week. The SOD value of 21% oxygen resuscitation was significantly higher than that of 50% 75% oxygen resuscitation (P0.05), and the SOD value of 100% oxygen resuscitation was significantly lower (P0.05). The SOD value of each resuscitation group decreased gradually with the increase of oxygen concentration. NF200 immunohistochemical staining in brain stem showed that the positive staining of TAI combined with hypoxia was significantly stronger than that of resuscitation group with 21% oxygen concentration at 24 h. The positive staining of TAI combined with hypoxia was significantly stronger than that of the resuscitation group at 21% oxygen concentration. Semi-quantitative analysis of staining positive axons showed that TAI combined with hypoxia at 24 h was significantly higher than the lowest at 21% oxygen concentration in each resuscitation group. The values of each group were significantly lower than that of the control group for 24 hours, and the value of TAI combined with hypoxia was still higher than that of the resuscitation group at 21% oxygen concentration. Conclusion 1. Oxygen administration can ameliorate hypoxemia and reduce the damage caused by lipid peroxidation in hypoxic rats after trauma. However, the therapeutic effects of different resuscitation oxygen concentrations were significantly different. The appropriate concentration of oxygen administration could significantly reduce the degree of axonal injury. Neuroprotective effect. 3. For this TAI combined with anoxic animal model, the concentration of short term oxygen resuscitation can not exceed 50%.
【學位授予單位】：上海交通大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R651.15
本文編號：2233042
[Abstract]:Objective 1. On the basis of (SBI) rat model of traumatic axonal injury (TAI) with hypoxia-induced secondary brain injury (SBI), different oxygen concentrations were resuscitated, and the changes of oxygen free radical index in brain homogenate of rats were analyzed and compared during different oxygen concentration resuscitation. To explore the effect of different oxygen concentration resuscitation on lipid peroxidation. On the basis of (SBI) rat model of traumatic axonal injury (TAI) combined with hypoxia-induced secondary brain injury (SBI), different oxygen concentration resuscitation was carried out, and the pathological changes of brain tissue were analyzed and compared between different oxygen concentration resuscitation. To investigate the effect of oxygen resuscitation on axonal damage. Different oxygen concentration resuscitation was carried out on the basis of traumatic axonal injury (TAI) combined with hypoxia-induced secondary brain injury (SBI) in rats, and the appropriate ventilation resuscitation strategy was explored. Method 1. The TAI model was made by using a self-made combined injury device. Hypoxemia was constructed by inhaling low concentration oxygen through a small animal ventilator, and then the rat model of TAI combined with hypoxemia was established. 2. On the basis of this model, the brain homogenate of rats after oxygen resuscitation was detected to study the effect of different oxygen concentration resuscitation on lipid peroxidation in rats. On the basis of this model, the effects of different oxygen concentration resuscitation on the pathological changes of rat brain tissue were studied by immunohistochemical staining of NF200 in brain tissue after oxygen resuscitation. Results 1. Compared with Sham group, MDA content in Tai H group and resuscitation group increased significantly at 24 h, and then returned to normal level at 1 week after resuscitation. Compared with TAI H group, MDA decreased during 50% oxygen resuscitation in each resuscitation group. There was significant difference between the two groups (P0.05), the SOD content in the resuscitation group gradually increased with the increase of oxygen concentration. The SOD content in the Tai H group and the resuscitation group decreased significantly at 24 hours, which was significantly lower than that in the Sham group (P0.05) at 1 week, and the SOD content in each resuscitation group was lower than that in the TAI H group at 1 week. The SOD value of 21% oxygen resuscitation was significantly higher than that of 50% 75% oxygen resuscitation (P0.05), and the SOD value of 100% oxygen resuscitation was significantly lower (P0.05). The SOD value of each resuscitation group decreased gradually with the increase of oxygen concentration. NF200 immunohistochemical staining in brain stem showed that the positive staining of TAI combined with hypoxia was significantly stronger than that of resuscitation group with 21% oxygen concentration at 24 h. The positive staining of TAI combined with hypoxia was significantly stronger than that of the resuscitation group at 21% oxygen concentration. Semi-quantitative analysis of staining positive axons showed that TAI combined with hypoxia at 24 h was significantly higher than the lowest at 21% oxygen concentration in each resuscitation group. The values of each group were significantly lower than that of the control group for 24 hours, and the value of TAI combined with hypoxia was still higher than that of the resuscitation group at 21% oxygen concentration. Conclusion 1. Oxygen administration can ameliorate hypoxemia and reduce the damage caused by lipid peroxidation in hypoxic rats after trauma. However, the therapeutic effects of different resuscitation oxygen concentrations were significantly different. The appropriate concentration of oxygen administration could significantly reduce the degree of axonal injury. Neuroprotective effect. 3. For this TAI combined with anoxic animal model, the concentration of short term oxygen resuscitation can not exceed 50%.
【學位授予單位】：上海交通大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R651.15
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