【摘要】：目的觀察微小RNA-200c(miR-200c)與酪氨酸激酶受體B(Trk B)腰椎間盤髓核組織中的表達(dá)變化,并探討其與腰椎間盤髓核退變程度的相關(guān)性。方法收集腰椎間盤髓核組織標(biāo)本60例,用Pfirmlann分級對髓核退變程度進(jìn)行分級,Ⅱ、Ⅲ、Ⅳ級各20例。采用細(xì)胞衰老β-半乳糖苷酶實(shí)驗(yàn)檢測不同分級髓核組織中衰老髓核細(xì)胞百分比,RT-PCR技術(shù)檢測髓核組織中miR-200c、Trk B mRNA表達(dá),Western blotting技術(shù)檢測髓核組織中Trk B蛋白表達(dá),采用Pearson相關(guān)分析miR-200c、Trk B與衰老髓核細(xì)胞的關(guān)系。結(jié)果 PfirmlannⅡ、Ⅲ、Ⅳ級髓核組織中衰老髓核細(xì)胞百分比、miR-200c相對表達(dá)量、Trk B mRNA及蛋白相對表達(dá)量兩兩比較,P均0.05。miR-200c mRNA與Trk B mRNA相對表達(dá)量呈負(fù)相關(guān)(r=-0.71,P0.05),miR-200c mRNA相對表達(dá)量與衰老髓核細(xì)胞百分比呈正相關(guān)(r=0.75,P0.05),Trk B mRNA相對表達(dá)量與衰老髓核細(xì)胞百分比呈負(fù)相關(guān)(r=-0.70,P0.05)。結(jié)論腰椎間盤髓核退變程度越高,miR-200c表達(dá)越高,Trk B mRNA表達(dá)越低;miR-200c可能通過下調(diào)Trk B表達(dá)參與腰椎間盤髓核組織的退變過程。
[Abstract]:Objective to observe the expression of small RNA-200c (miR-200c) and tyrosine kinase receptor (B (Trk B) in nucleus pulposus of lumbar disc and to explore the relationship between miR-200c and degeneration of nucleus pulposus of lumbar intervertebral disc. Methods 60 specimens of nucleus pulposus of lumbar intervertebral disc were collected. The grade of degeneration of nucleus pulposus was graded by Pfirmlann classification, including 20 cases of grade 鈪，

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