本文選題：細(xì)粒棘球蚴 + 體外實(shí)驗(yàn)��； 參考：《石河子大學(xué)》2015年碩士論文

【摘要】：目的:目前對(duì)包蟲(chóng)病的治療,手術(shù)仍然是主要的手段,但術(shù)中易造成包蟲(chóng)囊腫囊液外溢,以及未殺死的原頭節(jié)殘留于手術(shù)創(chuàng)面或腹腔,這是導(dǎo)致手術(shù)后復(fù)發(fā)最主要的原因,因此術(shù)中、術(shù)后應(yīng)用短時(shí)間內(nèi)殺死頭節(jié)又對(duì)周?chē)＝M織損傷小的藥物是阻止該病復(fù)發(fā)最常用的方法。本研究以體外培養(yǎng)的細(xì)粒棘球蚴原頭節(jié)為研究對(duì)象,觀察三氧化二砷對(duì)綿羊細(xì)粒棘球蚴原頭節(jié)生長(zhǎng)的影響,進(jìn)一步評(píng)價(jià)三氧化二砷用于臨床抗包蟲(chóng)治療的可能性,為三氧化二砷在肝包蟲(chóng)病治療的臨床應(yīng)用提供依據(jù)。方法:將新鮮的自然感染細(xì)粒棘球蚴的綿羊肝臟帶回實(shí)驗(yàn)室,用無(wú)菌方式抽取囊內(nèi)容物置培養(yǎng)瓶中,然后體外培養(yǎng)原頭節(jié)3天,待適應(yīng)體外環(huán)境后分別用含2μmol/L、4μmol/L、6μmol/L、8μmol/L濃度的三氧化二砷作用。用0.1%的伊紅溶液染色,在光學(xué)顯微鏡下觀察三氧化二砷不同時(shí)間、不同濃度對(duì)原頭節(jié)的活力的影響(死的原頭節(jié)會(huì)被染成紅色),實(shí)驗(yàn)單獨(dú)重復(fù)三次,根據(jù)數(shù)值繪制活力曲線。SEM觀察原頭節(jié)表面超微結(jié)構(gòu);TEM觀察原頭節(jié)內(nèi)部超微結(jié)構(gòu)的改變;用Caspase-3試劑盒檢測(cè)原頭節(jié)內(nèi)Caspase-3酶活性;Western blot檢測(cè)原頭節(jié)p-Akt蛋白表達(dá)水平。結(jié)果:體外孵育14d后,空白1640對(duì)照組原頭節(jié)的活力幾乎沒(méi)有改變。三氧化二砷各濃度組均對(duì)原頭節(jié)有殺傷作用,8μmol/L三氧化二砷濃度組殺傷作用強(qiáng)于其他濃度組。表面超微結(jié)構(gòu)出現(xiàn)凹陷,原頭節(jié)呈外翻,頂突破壞,內(nèi)部超微結(jié)構(gòu)變化有合胞體帶變薄、結(jié)構(gòu)松散,出現(xiàn)少量脂滴,微絨毛減少。各藥物作用組Caspase-3活性較正常對(duì)照組活性增加。Western blot結(jié)果顯示P-Akt的表達(dá)量隨藥物濃度及時(shí)間的增加而呈明顯下降趨勢(shì)。結(jié)論:1.三氧化二砷對(duì)細(xì)粒棘球蚴原頭節(jié)有殺傷作用。2.其作用機(jī)制可能是抑制了細(xì)粒棘球蚴原頭節(jié)中p-Akt的表達(dá),并可上調(diào)Caspase-3的表達(dá)而抑制生長(zhǎng)、促進(jìn)凋亡。
[Abstract]:Objective: at present, the treatment of hydatid disease is still the main method, but it is easy to cause hydatid cyst fluid spillover, and the non killed original joint is residual in the surgical wound or abdominal cavity, which is the main cause of recurrence after operation. Therefore, during the operation, the operation of the head joint in short time after operation is small in the surrounding normal tissue injury. The drug is the most commonly used method to prevent the recurrence of the disease. In this study, the effect of arsenic trioxide on the growth of echinococcosis in sheep was observed in vitro, and the possibility of arsenic trioxide used in the treatment of echinococcosis was further evaluated, and the treatment of arsenic trioxide in the liver hydatid disease was in the face of the treatment of liver hydatid disease. Methods: the sheep liver of the fresh and natural Echinococcus granulosus was brought back to the laboratory, and the capsule content was extracted with aseptic method, and then the original joint was cultured in vitro for 3 days. After the environment was adapted to the environment, the arsenic trioxide containing 2 mol/L, 4, mol/L, 6 u mol/L and 8 mu mol/L was used respectively. 0.1% Yi Hong was used. The effect of arsenic trioxide at different time and different concentration on the vitality of the original joint was observed under the optical microscope (the original joint was dyed red). The experiment was repeated three times alone, and the surface ultrastructure of the original joint was observed by the numerical activity curve.SEM. The changes of the inner ultrastructure of the original joint were observed by TEM; Caspase-3 The Caspase-3 enzyme activity in the original joint was detected by the kit and the expression level of the p-Akt protein was detected by Western blot. Results: after incubating for 14d in vitro, the activity of the original joint in the blank 1640 control groups was almost unchanged. The concentrations of arsenic trioxide all had a killing effect on the original joint, and the killing effect of the 8 micron arsenic trioxide concentration group was stronger than the other concentration. The surface ultrastructure appeared sunken, the original joint was ectropion, the apical process was destroyed, the inner ultrastructure changed with the syncytial body thinning, the structure loosened, a small amount of lipid droplets appeared, and the microvilli decreased. The activity of Caspase-3 in each drug group was increased by.Western blot results, which showed that the expression of P-Akt was increased with the increase of drug concentration and time. Conclusion: 1. arsenic trioxide has a killing effect on Echinococcus granulosus (Echinococcus granulosus).2., which may inhibit the expression of p-Akt in Echinococcus granulosus, and increase the expression of Caspase-3 to inhibit growth and promote apoptosis.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：R657.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 周慧娟;一組新的有機(jī)砷劑對(duì)彭亨絲蟲(chóng)的殺蟲(chóng)作用[J];國(guó)外醫(yī)學(xué)(寄生蟲(chóng)病分冊(cè));1987年04期

2 李淑紅;王京仁;黃春紅;;復(fù)方中藥油劑治療野豬疥螨病的療效試驗(yàn)[J];黑龍江畜牧獸醫(yī);2005年12期

3 趙艷宇;朱楊;李德超;李京玲;梁香花;王玉龍;龐靖;周彥鵬;;三氧化二砷納米粒對(duì)舌鱗癌細(xì)胞PI3K/AKT通路的影響[J];口腔醫(yī)學(xué)研究;2014年07期

4 李長(zhǎng)明;;砷劑的藥理作用及其臨床應(yīng)用[J];遼寧畜牧獸醫(yī);1979年01期

5 姚秀娟;王米;江善祥;薛飛群;;有機(jī)胂制劑在畜禽生產(chǎn)中的作用、毒性及環(huán)境行為[J];獸藥與飼料添加劑;2009年06期

6 徐三元，徐仁達(dá)，陳仁爾，陳衛(wèi)星;經(jīng)濟(jì)高效多功能飼料添加劑──洛克沙生[J];飼料博覽;1996年04期

7 汪志錚;;火雞黑頭病的防治[J];飼料研究;1986年01期

8 趙雪強(qiáng);王績(jī)英;王昌明;莫碧文;蔣明;陳峰;;三氧化二砷增強(qiáng)TRAIL殺傷人A549細(xì)胞的作用及機(jī)制研究[J];中國(guó)藥理學(xué)通報(bào);2011年03期

9 操繼躍;;抗球蟲(chóng)藥物研究進(jìn)展與應(yīng)用技術(shù)[J];中國(guó)家禽;2009年14期

10 梁國(guó)剛,張啟偉;朱砂、雄黃中各成分的溶解度對(duì)其藥效、毒副作用的影響[J];中國(guó)中藥雜志;2002年05期

相關(guān)博士學(xué)位論文 前1條

1 盧建軍;納米級(jí)對(duì)氨基苯胂酸微粒對(duì)斷奶仔豬的抗菌促生長(zhǎng)效果及其機(jī)理研究[D];浙江大學(xué);2006年

相關(guān)碩士學(xué)位論文 前1條

1 周春景;日本血吸蟲(chóng)miR-2a靶基因的鑒定及信號(hào)分子Akt和GSK3的初步研究[D];中國(guó)農(nóng)業(yè)科學(xué)院;2012年

，

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