本文選題：脊髓損傷 + 認知障礙��； 參考：《第三軍醫(yī)大學學報》2017年01期

【摘要】：目的探究脊髓損傷后的認知、情緒改變以及腦內(nèi)相關區(qū)域病理變化和內(nèi)質(zhì)網(wǎng)應激相關分子表達情況。方法 120只成年SD雌性大鼠分為損傷組和對照組(每組60只)。制備脊髓T10節(jié)段的Allen法打擊損傷模型(打擊力度20 g×25 mm),對照組只接受椎板切除術。BBB評分觀察損傷后運動功能恢復情況,Morris水迷宮和高架十字迷宮分別檢測大鼠學習記憶功能和情緒改變。尼氏染色觀察大腦神經(jīng)細胞形態(tài)數(shù)量,快速高爾基體染色檢測海馬區(qū)域樹突棘密度。Western blot檢測GRP78和Caspase-12蛋白在海馬區(qū)域的表達變化。結果高架十字迷宮檢測損傷大鼠進入開臂次數(shù)明顯減少,水迷宮空間探索測試結果顯示損傷動物跨越平臺次數(shù)明顯下降(P0.05,P0.01)。尼氏染色和快速高爾基體染色結果顯示大鼠皮質(zhì)和海馬區(qū)域神經(jīng)元形態(tài)和數(shù)量異常,海馬區(qū)域樹突棘密度下降(P0.05,P0.01)。Western blot檢測結果顯示脊髓損傷后內(nèi)質(zhì)網(wǎng)應激相關蛋白GRP78表達上調(diào)(P0.05,P0.01)。結論脊髓損傷后成年大鼠產(chǎn)生持續(xù)學習記憶能力下降,同時出現(xiàn)焦慮的情緒變化。這可能與脊髓損傷后海馬和皮質(zhì)區(qū)域發(fā)生的內(nèi)質(zhì)網(wǎng)應激引起海馬與皮質(zhì)區(qū)域神經(jīng)細胞受損有關。
[Abstract]:Objective to investigate the changes of cognition, emotion, pathological changes and ER stress related molecules in brain after spinal cord injury (sci). Methods 120 adult SD female rats were divided into injury group and control group (60 rats in each group). Establishment of spinal cord T10 injury model with Allen method (20 g 脳 25 mm). The control group only received laminectomy. BBB score was used to observe the recovery of motor function after injury. Morris water maze and elevated cross maze were used to detect the learning of rats. Memory function and emotional changes. The morphological number of neural cells was observed by Nissl's staining and the expression of GRP78 and Caspase-12 proteins in the hippocampus was detected by rapid Golgi body staining and the density of dendritic spine in the hippocampal region. Western blot was used to detect the expression of GRP78 and Caspase-12 protein in the hippocampus. Results the number of entry into the open arm of the injured rats was significantly reduced by elevated cross maze test. The results of space exploration of water maze showed that the number of cross platform crossing of the injured animals was significantly decreased (P 0.05) and P 0.01 (P 0.01). The results of Nissl staining and rapid Golgi body staining showed that the morphology and number of neurons in cortex and hippocampus of rats were abnormal, and the density of dendritic spine in hippocampus was decreased. Western blot analysis showed that the expression of ERS-associated protein GRP78 up-regulated P0.05 and P0.01a after spinal cord injury. Conclusion after spinal cord injury, adult rats have decreased ability of continuous learning and memory and anxiety. This may be related to endoplasmic reticulum stress in hippocampus and cortex after spinal cord injury.
【作者單位】： 重慶醫(yī)科大學附屬第二醫(yī)院康復醫(yī)學科;第三軍醫(yī)大學大坪醫(yī)院野戰(zhàn)外科研究所第三研究室創(chuàng)傷與燒傷國家重點實驗室;
【基金】：國家自然科學基金面上項目(81272159) 重慶市教委科研項目(KJ1500237)~~
【分類號】：R651.2

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