本文選題：腫瘤壞死因子-α + 骨性關(guān)節(jié)炎�。� 參考：《中國(guó)骨質(zhì)疏松雜志》2017年10期

【摘要】：目的觀察不同劑量腫瘤壞死因子-α(tumor necrosis factor,TNF-α)在構(gòu)建大鼠骨性關(guān)節(jié)炎(osteoarthritis,OA)模型中對(duì)內(nèi)質(zhì)網(wǎng)應(yīng)激的影響,初步探討大鼠骨性關(guān)節(jié)炎病理過(guò)程中內(nèi)質(zhì)網(wǎng)應(yīng)激的作用。方法將20只SPF級(jí)SD雄性大鼠隨機(jī)分為健康對(duì)照組、TNF-α1ng/m L干預(yù)組、TNF-α5ng/m L干預(yù)組和TNF-α10ng/m L干預(yù)組,分別給予不同劑量TNF-α處理,4周后取材,培養(yǎng)提取的各組原代軟骨細(xì)胞,采用MTT法檢測(cè)不同劑量TNF-α對(duì)軟骨細(xì)胞活力的影響;ELISA法檢測(cè)內(nèi)皮細(xì)胞粘附分子-1(endothelial cell adhesion molecule-1,s ICAM-1)含量變化,TUNEL法檢測(cè)各組軟骨細(xì)胞凋亡率,蛋白印跡法(Western blot)檢測(cè)炎癥因子MMP9、ICAM1的表達(dá)以及內(nèi)質(zhì)網(wǎng)應(yīng)激指標(biāo)IRE1、p-IRE1、GRP78的水平變化。結(jié)果 MTT法檢測(cè)提示加入不同劑量的TNF-α后軟骨細(xì)胞活力未見(jiàn)明顯改變;TUNEL法檢測(cè)結(jié)果提示,與對(duì)照組相比,TNF-α干預(yù)組軟骨細(xì)胞凋亡率較高(P0.01),并呈濃度依賴(lài)性促進(jìn)細(xì)胞凋亡;Western blot結(jié)果表明,與對(duì)照組相比,軟骨細(xì)胞的p-IRE1、IRE1、GRP78、MMP9及ICAM1的表達(dá)水平呈TNF-α濃度依賴(lài)性升高(P0.01)。結(jié)論在TNF-α構(gòu)建大鼠骨性關(guān)節(jié)炎模型中,炎癥因子與內(nèi)質(zhì)網(wǎng)應(yīng)激指標(biāo)均發(fā)生顯著改變,內(nèi)質(zhì)網(wǎng)應(yīng)激可能在大鼠骨性關(guān)節(jié)炎的發(fā)生發(fā)展過(guò)程中發(fā)揮重要影響,且當(dāng)TNF-α濃度為10 ng/m L時(shí),其對(duì)炎性因子及內(nèi)質(zhì)網(wǎng)指標(biāo)的作用效果最為顯著。
[Abstract]:Objective to observe the effects of different doses of tumor necrosis factor- 偽 on endoplasmic reticulum stress in rat osteoarthritis model, and to explore the role of endoplasmic reticulum stress in the pathological process of rat osteoarthritis. Methods Twenty Sprague-SPF male rats were randomly divided into two groups: TNF- 偽 1ng/m L intervention group and TNF- 偽 10ng/m L intervention group. The primary chondrocytes of each group were cultured after 4 weeks of treatment with different doses of TNF- 偽 10ng/m. The effect of different doses of TNF- 偽 on chondrocyte viability was detected by MTT assay. The changes of the content of endothelial cell adhesion molecule cell adhesion molecule-1and ICAM-1 were detected by Elisa. The apoptosis rate of chondrocytes in each group was detected by Tunel method. Western blot was used to detect the expression of ICAM1 and the level of endoplasmic reticulum stress (IRE1) -IRE1 / GRP78. Results MTT assay showed that the activity of chondrocytes did not change after adding different doses of TNF- 偽. Compared with the control group, the apoptotic rate of chondrocytes in the TNF- 偽 intervention group was higher than that in the control group. The results showed that the expression of p-IRE1, IRE1, GRP78, MMP9 and ICAM1 in chondrocytes was increased in a concentration-dependent manner in a concentration dependent manner (P0.01a), and the results showed that the expression levels of p-IRE1, IRE1, GRP78, MMP9 and ICAM1 in chondrocytes were increased in a concentration-dependent manner. Conclusion the inflammatory factors and endoplasmic reticulum stress were significantly changed in rat osteoarthritis induced by TNF- 偽. Endoplasmic reticulum stress may play an important role in the development of osteoarthritis in rats. When the concentration of TNF- 偽 was 10 ng/m / L, the effect of TNF- 偽 on inflammatory factors and endoplasmic reticulum was the most significant.
【作者單位】： 蘭州軍區(qū)總醫(yī)院全軍骨科中心關(guān)節(jié)外科;寧夏醫(yī)科大學(xué);第四軍醫(yī)大學(xué)西京醫(yī)院老年病科;
【基金】：國(guó)家自然科學(xué)基金(81371983) 甘肅省青年科技基金(1606RJYA300) 甘肅省自然科學(xué)基金(1606RJZA208) 甘肅省科技支撐計(jì)劃(S04671)
【分類(lèi)號(hào)】：R-332;R684.3

【相似文獻(xiàn)】

相關(guān)期刊論文 前1條

1 葛廣勇;趙建寧;劉剛;;膝骨性關(guān)節(jié)炎模型的分期特征[J];中國(guó)臨床康復(fù);2006年04期

，

本文編號(hào)：2010299