本文選題：脊髓損傷 + 星形膠質(zhì)細(xì)胞��； 參考：《杭州師范大學(xué)》2015年碩士論文

【摘要】：在中樞神經(jīng)系統(tǒng)中,少突膠質(zhì)細(xì)胞是一類(lèi)包裹在神經(jīng)元軸突形成髓鞘化的神經(jīng)細(xì)胞,在脊髓損傷以后,大部分的少突膠質(zhì)細(xì)胞細(xì)胞和神經(jīng)元都?jí)乃?接著星形膠質(zhì)細(xì)胞大量增殖形成膠質(zhì)疤痕。膠質(zhì)疤痕會(huì)阻礙神經(jīng)元的再修復(fù),以及髓鞘的修復(fù)。我們想研究OPC在脊髓損傷后,如何參與脊髓損傷后的自我修復(fù)。并且在以往的研究中,通過(guò)移植少突膠質(zhì)細(xì)胞細(xì)胞前體來(lái)治療脊髓損傷時(shí),發(fā)現(xiàn)大部分的少突膠質(zhì)細(xì)胞前體在體內(nèi)分化成為星形膠質(zhì)細(xì)胞。我們推測(cè)在脊髓損傷以后,體內(nèi)微環(huán)境發(fā)生了改變,可能產(chǎn)生了部分因子,影響少突膠質(zhì)細(xì)胞前體分化成為星形膠質(zhì)細(xì)胞。我們通過(guò)PDGFRa-creER/Rosa-td-tTomato小鼠為研究對(duì)象,PDGFRa為少突膠質(zhì)細(xì)胞前體的標(biāo)志蛋白,通過(guò)tomato標(biāo)記后,研究脊髓損傷以后,對(duì)少突膠質(zhì)細(xì)胞前體發(fā)育的影響。我們發(fā)現(xiàn)在脊髓損傷后,被Tomato信號(hào)標(biāo)記的細(xì)胞,一部分表達(dá)cc1陽(yáng)性,然后有一小部分細(xì)胞表達(dá)GFAP陽(yáng)性。表明大部分OPC參與形成髓鞘的修復(fù),但是有一小部分OPC分化成為星形膠質(zhì)細(xì)胞形成膠質(zhì)疤痕。
[Abstract]:In the central nervous system, oligodendrocytes are a group of nerve cells that form myelination around the axons of neurons. After spinal cord injury, most of the oligodendrocytes and neurons are necrotic. Astrocytes then proliferate in large numbers to form glial scars. Glial scars block the rerepair of neurons and the repair of myelin sheath. We want to study how OPC participates in self-repair after spinal cord injury. In previous studies, it was found that most of the oligodendrocyte precursors differentiated into astrocytes in vivo after transplantation of oligodendrocyte precursors to treat spinal cord injury. We speculate that the microenvironment changes after spinal cord injury, which may produce some factors and influence the differentiation of oligodendrocytes into astrocytes. In this study, PDGFRa-creER/Rosa-td-tTomato mouse was used as a marker protein of oligodendrocyte precursor. The effects of tomato labeling on the development of oligodendrocyte precursor were studied after spinal cord injury. We found that after spinal cord injury, some cells labeled with Tomato signal expressed cc1 positive, and then a small number of cells expressed GFAP positive. It was suggested that most of OPC were involved in the repair of myelin sheath, but a small number of OPC differentiated into astrocytes to form glial scars.
【學(xué)位授予單位】：杭州師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：R651.2

【共引文獻(xiàn)】

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1 涂雪松;;神經(jīng)干細(xì)胞移植治療神經(jīng)疾病機(jī)制的基礎(chǔ)研究[J];神經(jīng)損傷與功能重建;2014年06期

2 Justin A.Beller;Diane M.Snow;;Proteoglycans: Road Signs for Neurite Outgrowth[J];Neural Regeneration Research;2014年04期

3 Zoe Zhang;Yi Ping Zhang;Lisa B.E.Shields;Christopher B.Shields;;Technical comments on rodent spinal cord injuries models[J];Neural Regeneration Research;2014年05期

4 Miranda Mladinic;Andrea Nistri;;Dynamic expression of ATF3 as a novel tool to study activation and migration of endogenous spinal stem cells and their role in neural repair[J];Neural Regeneration Research;2015年05期

相關(guān)博士學(xué)位論文 前1條

1 刁永鵬;神經(jīng)因素對(duì)缺血肢體血管生成和骨骼肌纖維重塑影響的研究[D];北京協(xié)和醫(yī)學(xué)院;2015年

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本文編號(hào)：1966100