本文選題：體外灌注 + 去細(xì)胞化　； 參考：《中國生物醫(yī)學(xué)工程學(xué)報》2017年03期

【摘要】：研究采用體外灌注法制備腎和肺去細(xì)胞器官支架的最優(yōu)方法。將10只成年SD雄鼠作為器官支架供體,進(jìn)行去細(xì)胞器官支架制備,不同器官采用不同血液循環(huán)方法進(jìn)行體外大氣壓灌注(基于大氣壓力往循環(huán)體系中注入)0.75%SDS、1%Triton X-100、0.1%H_2O_2制備器官去細(xì)胞支架,通過HE染色、DAPI熒光染色方法檢測去細(xì)胞程度,通過掃描電鏡觀察其結(jié)構(gòu)脈絡(luò)網(wǎng)和去細(xì)胞程度,通過阿利新藍(lán)-過碘酸雪夫氏染法(AB-PAS)、Masson染色、免疫組化染色方法檢測去細(xì)胞器官支架的成分保留。結(jié)果表明,采用體外大氣壓灌注法制備的去細(xì)胞器官支架能有效去除細(xì)胞,且糖原、膠原、纖連蛋白(Fibronectin)、彈性蛋白(Elastin)、層粘連蛋白(Laminin)、IV型膠原(Collagen IV)等有效保留,同時支架能保持良好的結(jié)構(gòu)脈絡(luò)網(wǎng)。通過大氣壓注入去細(xì)胞化試劑,能有效保留良好的結(jié)構(gòu)脈絡(luò)網(wǎng),同時達(dá)到有效的去細(xì)胞程度(去細(xì)胞程度大于99%),且營養(yǎng)成分能有效保留。
[Abstract]:To study the optimal method of preparing renal and lung acellular organ scaffolds by in vitro perfusion. Ten adult Sprague-Dawley rats were used as scaffolds for the preparation of acellular organ scaffolds. Different organs used different blood circulation methods for extracorporeal atmospheric pressure perfusion (based on atmospheric pressure, 0.75 was injected into the circulation system, and one Triton X-1000.1H _ 2O _ 2 was used to prepare organ removal scaffolds, and the degree of acellular cells was detected by HE staining and DAPI fluorescence staining. The structural choroid network and acellular degree were observed by scanning electron microscope. The components of the scaffold were detected by immunohistochemical staining and AB-PASA Masson staining. The results showed that acellular organ scaffolds prepared by in vitro atmospheric pressure perfusion could effectively remove cells, and glycogen, collagen, fibronectinine, elastin, lamininine IV collagen and laminin IV collagen were effectively retained. At the same time, the scaffold can maintain a good structure of the network. Through atmospheric pressure injection of decellular reagents, good structural choroid net can be effectively retained, and the effective degree of acellular (the degree of acellular is greater than 99%), and the nutrient components can be effectively retained at the same time.
【作者單位】： 溫州醫(yī)科大學(xué)藥學(xué)院;
【基金】：國家自然科學(xué)基金(81571392) 浙江省衛(wèi)生高層次創(chuàng)新人才 溫州市551高層次人才創(chuàng)新技術(shù)項(xiàng)目
【分類號】：R318.08;R617

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