本文選題：重水　切入點(diǎn)：骨骼肌　出處：《生理學(xué)報(bào)》2017年03期

【摘要】：本研究旨在以重水(~2H_2O)為示蹤劑,測(cè)定大鼠骨骼肌蛋白合成動(dòng)力學(xué)相關(guān)參數(shù)。將20只Sprague Dawley(SD)大鼠以腹腔注射、日常飲水的形式標(biāo)記~2H_2O,分別在首次標(biāo)記后的第1、3、5、6和10周,各取4只大鼠心臟穿刺取血漿,分離股四頭肌。提取骨骼肌蛋白和血漿游離氨基酸,用鹽酸水解,衍生后用氣相色譜-質(zhì)譜儀(gas chromatography-mass spectrometry,GC-MS)檢測(cè)骨骼肌蛋白2~H-丙氨酰基和血漿游離丙氨酸中~2H的豐度,計(jì)算骨骼肌蛋白的分?jǐn)?shù)合成率、合成動(dòng)力學(xué)方程。結(jié)果顯示,骨骼肌蛋白的分?jǐn)?shù)合成率為12.8%/周,合成動(dòng)力學(xué)方程為f_t=0.158×(1-e~(-0.128t))。以上結(jié)果提示,重水標(biāo)記法能有效測(cè)定骨骼肌蛋白的合成動(dòng)力學(xué)參數(shù),適用于長(zhǎng)期標(biāo)記實(shí)驗(yàn)。
[Abstract]:The purpose of this study was to determine the parameters related to the synthesis kinetics of skeletal muscle protein in rats using 2H _ 2O as tracer. Twenty Sprague Dawley Dawley SD rats were injected intraperitoneally and labeled with 2H _ 2O in the form of daily drinking water. Plasma was extracted from each of 4 rats by heart puncture, and quadriceps femoris was isolated. Skeletal muscle protein and plasma free amino acids were extracted and hydrolyzed with hydrochloric acid. After derivation, the abundance of 2H-alanine in skeletal muscle protein and free alanine in plasma were detected by gas chromatography-mass spectrometer (chromatography-mass). The fractional synthesis rate and synthesis kinetics equation of skeletal muscle protein were calculated. The fraction synthesis rate of skeletal muscle protein was 12.8g / week, and the synthesis kinetics equation was f_t=0.158 脳 1-eosin -0.128tl. These results suggest that the heavy water labeling method can effectively determine the synthesis kinetic parameters of skeletal muscle protein, and it is suitable for long-term labeling experiment.
【作者單位】： 揚(yáng)州大學(xué)旅游烹飪學(xué)院營(yíng)養(yǎng)系;
【基金】：supported by the National Natural Science Foundation of China(No.81472963)
【分類號(hào)】：R68

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