本文選題：miR-a　切入點：認知功能障礙　出處：《臨床麻醉學雜志》2017年10期

【摘要】：目的觀察術(shù)后認知功能障礙(postoperative cognitive dysfunction,POCD)小鼠術(shù)后血清、海馬及前額葉皮質(zhì)中miR-146a表達的變化。方法雄性C57BL/6小鼠50只,12~14周齡,體重25~30g,采用隨機數(shù)字表法將其分為兩組:對照組(C組)和麻醉手術(shù)組(AS組),每組25只。AS組在2.1%異氟醚吸入麻醉下行脛骨骨折開放復(fù)位內(nèi)固定術(shù),C組不行麻醉和手術(shù)處理。于術(shù)前24h及術(shù)后6、12、24、48h每組分別取3只小鼠進行摘眼球取血,處死后取海馬和前額葉皮質(zhì),采用RT-PCR法測小鼠血清、海馬和前額葉皮質(zhì)中miR-146a表達量。于術(shù)后第1、3、7天每組各取10只小鼠,進行場景恐懼記憶實驗和聲音提示恐懼記憶實驗,記錄僵直時間百分比。結(jié)果與C組比較,AS組場景恐懼記憶實驗中僵直時間百分比明顯降低(P0.05),聲音提示恐懼記憶實驗中兩組僵直時間百分比差異無統(tǒng)計學意義。在血清中,與C組比較,術(shù)后6、12、24、48h AS組miR-146a表達量明顯升高(P0.05);與術(shù)后12h比較,術(shù)后6、24、48hAS組miR-146a表達量明顯降低(P0.05)。在海馬中,與C組比較,術(shù)后6、12、24、48h AS組miR-146a表達量明顯升高(P0.05);與術(shù)后12h比較,術(shù)后6、48h AS組miR-146a表達量明顯降低(P0.05)。在前額葉皮質(zhì)中,與C組比較,AS組術(shù)后24、48h miR-146a表達量明顯升高(P0.05);與術(shù)后24h比較,術(shù)后48h AS組miR-146a表達量明顯升高(P0.05)。結(jié)論 POCD小鼠血清、海馬及前額葉皮質(zhì)中miR-146a表達升高,miR-146a表達量的變化可能與POCD的發(fā)病機制相關(guān)。
[Abstract]:Objective to observe the changes of miR-146a expression in serum, hippocampus and prefrontal cortex of postoperative cognitive dysfunctional POCD mice after operation. The body weight was 2530g. It was randomly divided into two groups: control group (C group) and anaesthesia operation group (as group). 25 rats in each group were treated with open reduction and internal fixation of tibial fracture under 2.1% isoflurane inhalation anesthesia. After 24 hours of operation, 3 mice in each group were taken for eyeball extraction, and blood was taken from each group 24 hours after operation. The expression of miR-146a in serum, hippocampus and prefrontal cortex of mice was measured by RT-PCR method. Ten mice in each group were collected on the 1st day after operation. Results compared with group C, the percentage of stiffness time in as group was significantly lower than that in group C (P 0.05). The results showed that there was no significant difference in percentage of stiffness time between the two groups in fear memory experiment. Compared with group C, the expression of miR-146a in as group was significantly higher than that in group C, and the expression of miR-146a in group as was significantly lower than that in group C at 12 h after operation. In hippocampus and group C, the expression of miR-146a in group as was significantly higher than that in group C, and the expression of miR-146a in group as was significantly higher than that in group C at 12 h after operation, and was significantly higher than that in group C at 12 h after operation, and at 12 h after operation, the expression of miR-146a in as group was significantly lower than that in group C at 12 h after operation, and compared with that in group C at 12 h after operation. In the prefrontal cortex, the expression of miR-146a in the prefrontal cortex was significantly increased at 24 hours after operation in as group, and the expression of miR-146a in as group was significantly higher than that in 24 h after operation at 24 h after operation. Conclusion the expression of miR-146a in as group was significantly higher than that in control group at 24 h after operation. Conclusion the expression of miR-146a in the serum of POCD mice is significantly higher than that in the control group. The increase of miR-146a expression in hippocampus and prefrontal cortex may be related to the pathogenesis of POCD.
【作者單位】： 武漢大學中南醫(yī)院麻醉科;
【基金】：國家自然科學基金(81371195) 武漢市中青年醫(yī)學骨干人才培養(yǎng)工程項目(2014年)
【分類號】：R614

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本文編號：1685217