本文選題：地塞米松　切入點：肌肉衰減綜合征　出處：《錦州醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的通過研究地塞米松誘導(dǎo)C57BL/6小鼠產(chǎn)生肌肉質(zhì)量和肌肉功能的變化,并分析小鼠骨骼肌中相關(guān)分子表達(dá)水平的變化,探究得到理想的肌肉衰減綜合征小鼠的建模方法。方法將35只6~7月齡C57BL/6雄性小鼠分為3組:C組(對照組:0.9%生理鹽水)、L組(低劑量組:5 mg/kg地塞米松磷酸鈉注射液),H組(高劑量組:10 mg/kg地塞米松磷酸鈉注射液),連續(xù)皮下注射6周。前2周每3 d以及后4周每7 d測量一次小鼠攝食量、體重和體成分。最后一次給藥24 h后,利用水迷宮測量小鼠的游泳速度和輪式跑臺測量小鼠的掉落次數(shù),綜合評價小鼠的肌肉功能。肌肉功能測試結(jié)束后取小鼠腓腸肌,利用熒光定量PCR的方法檢測三組小鼠骨骼肌中Atrogin-1、Mu RF1、Myo D和myogenin m RNA的表達(dá)水平的差異。結(jié)果(1)從造模第1天開始,L組和H組平均每日攝食量較對照組顯著增加(P0.005);(2)L組造模第28天和H組造模第21天出現(xiàn)體重較C組顯著增加(P0.005)。(3)體成分方面,L組和H組造模第28天出現(xiàn)肌肉質(zhì)量較C組顯著降低(P0.005);L組和H組造模第6天出現(xiàn)脂肪含量較C組顯著增加(P0.005)。(4)肌肉功能方面,L組和H組平均游泳速度較C組明顯下降(P0.05),平均輪式跑臺掉落次數(shù)較C組明顯增多(P0.05)。(5)L組和H組骨骼肌中Atrogin-1和Mu RF1 m RNA表達(dá)較C組均明顯升高(P0.05);L組骨骼肌Myo D和Myogenin m RNA表達(dá)較C組沒有差異,H組骨骼肌中Myo D和Myogenin m RNA表達(dá)較C組明顯升高(P0.05)。結(jié)論通過皮下注射地塞米松磷酸鈉,能夠建立理想的肌肉衰減綜合征小鼠模型。
[Abstract]:Objective to study the changes of muscle mass and muscle function induced by dexamethasone in C57BL / 6 mice and to analyze the expression level of related molecules in skeletal muscle of mice. To explore an ideal method for modeling muscle attenuation syndrome mice, 35 C57BL / 6 male mice aged 6 to 7 months were divided into three groups: group C (control group: 0.9% saline) (low dose group: 5 mg/kg dexamethasone sodium phosphate injection). Group A (high dose group: 10 mg/kg dexamethasone sodium phosphate injection) was injected subcutaneously for 6 weeks, and the food intake of mice was measured every 3 days in the first 2 weeks and every 7 days in the last 4 weeks. Body weight and body composition. After the last administration for 24 hours, the swimming speed of mice was measured by water maze and the falling times of mice were measured by wheeled running platform, and the muscle function of mice was evaluated synthetically. The gastrocnemius muscle of mice was taken after the muscle function test. Fluorescence quantitative PCR was used to detect the expression of Atrogin-1Mu RF1D and myogenin m RNA in skeletal muscle of three groups of mice. Results from the first day of modeling, the average daily intake of Atrogin-1Mu RF1D and myogenin m RNA in group L and group H was significantly higher than that in group B (P 0.005) and group H was significantly higher than that in control group (P 0.005). Body mass in group L and group H on day 28 and group H were significantly lower than those in group C on day 28 and group H were significantly lower than those in group C and group H on day 6. The average swimming speed of group L and group H was significantly lower than that of group C (P 0.05), and the fall times of average wheel running platform were significantly higher than that of group C (P 0.05). The expression of Atrogin-1 and Mu RF1 m RNA in skeletal muscle of group L and H were significantly higher than that of group C. There was no difference in the expression of Myo D and Myogenin m RNA between the two groups. Conclusion the expression of Myo D and Myogenin m RNA in skeletal muscle of H group was significantly higher than that of C group. Conclusion Dexamethasone sodium phosphate can be injected subcutaneously into the skeletal muscle. An ideal mouse model of muscle attenuation syndrome can be established.
【學(xué)位授予單位】：錦州醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R685;R-332

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本文編號：1629431