本文選題：棕櫚酸　切入點(diǎn)：骨骼肌干細(xì)胞　出處：《南京醫(yī)科大學(xué)學(xué)報(bào)(自然科學(xué)版)》2017年05期 　論文類型：期刊論文

【摘要】：目的 :觀察棕櫚酸(palmitic acid,PA)對(duì)小鼠骨骼肌干細(xì)胞分化及功能的影響,為治療衰老相關(guān)的肌少癥尋找新思路。方法:對(duì)小鼠骨骼肌干細(xì)胞誘導(dǎo)分化后,同時(shí)予PA(100μmol/L)干預(yù),用熒光染色法、熒光定量PCR測(cè)定成肌基因肌球蛋白重鏈(myosin heavy chains,MyHC)表達(dá);用seahorse能量代謝儀檢測(cè)細(xì)胞的氧耗增加率,熒光定量PCR檢測(cè)線粒體融合基因線粒體融合素基因1(mitofusin1,Mfn1)、視神經(jīng)萎縮癥蛋白基因(opticatrophy 1,Opa1)和裂解基因發(fā)動(dòng)相關(guān)因子基因(dynamin 1-like,Drp1)、線粒體分裂素基因1(fission 1,Fis1)的相對(duì)表達(dá)量。結(jié)果 :PA刺激后,MyHC熒光表達(dá)量下降,肌小管融合率減低,同時(shí)seahorse能量代謝儀檢測(cè)細(xì)胞的氧耗增加率(oxygen consumption rate,OCR)降低(P0.05);線粒體融合基因無(wú)明顯異常,但裂解基因表達(dá)增加,融合/裂解比值下降(P0.05)。結(jié)論:游離脂肪酸抑制骨骼肌干細(xì)胞分化、影響線粒體氧耗功能,這可能是通過(guò)影響線粒體Drp1基因,增加線粒體裂解實(shí)現(xiàn)的。
[Abstract]:Objective: To observe the effect of palmitic acid (palmitic, acid, PA) on mice skeletal muscle stem cell differentiation and function, for the treatment of age-related sarcopenia in search of new ideas. Methods: mouse skeletal muscle stem cells after differentiation, and treated with PA (100 mol/L) intervention, using fluorescence staining, fluorescence quantitative determination of PCR muscle myosin heavy chain gene (myosin heavy chains, MyHC) expression; energy metabolism of seahorse cells was assessed with the increase in oxygen consumption rate, fluorescence quantitative PCR detection of mitochondrial fusion gene mitofusin 1 genes (mitofusin1, Mfn1), optic nerve atrophy protein gene (opticatrophy 1, Opa1) and a lytic gene related genes (dynamin, 1-like, Drp1) mitochondrial fission gene 1 (fission 1, Fis1). Results: the relative expression of PA after stimulation with MyHC fluorescence expression decreased, myotubule fusion rate reduce, and energy metabolism of seahorse detected by fine Cellular oxygen consumption rate of increase (oxygen consumption rate, OCR) decreased (P0.05); mitochondrial fusion gene had no obvious abnormalities, but lytic gene expression increased, fusion / cracking ratio decreased (P0.05). Conclusion: free fatty acids inhibit skeletal muscle stem cell differentiation, affecting mitochondrial oxygen consumption function, which may be affected by mitochondria Drp1 gene, increased mitochondrial fission is achieved.

【作者單位】： 南京醫(yī)科大學(xué)第一附屬醫(yī)院老年醫(yī)學(xué)科;
【基金】：國(guó)家自然科學(xué)基金面上項(xiàng)目(81370950)
【分類號(hào)】：R685

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本文編號(hào)：1581068