本文選題：吡咯烷二硫代氨基甲酯(PDTC)　切入點(diǎn)：頸椎間盤(pán)退變　出處：《中國(guó)藥理學(xué)通報(bào)》2017年10期 　論文類(lèi)型：期刊論文

【摘要】：目的觀(guān)察吡咯烷二硫代氨基甲酯(PDTC)對(duì)大鼠頸椎動(dòng)力失衡模型頸椎間盤(pán)組織形態(tài)及腫瘤壞死因子α(TNF-α)、基質(zhì)金屬蛋白酶9(MMP-9)表達(dá)的影響,探討PDTC抑制頸椎間盤(pán)退變作用及其機(jī)制。方法 54只SD大鼠隨機(jī)分為3組,切斷大鼠頸后部淺、深肌群構(gòu)建頸椎間盤(pán)退變動(dòng)物模型。PDTC給藥組(A組):造模術(shù)后腹腔每日注射PDTC溶液。模型組(B組):造模術(shù)后腹腔每日注射生理鹽水。假手術(shù)組(C組):手術(shù)切開(kāi)大鼠頸后部皮膚后立即縫合并每日腹腔注射生理鹽水。術(shù)后10、12、16周分批處死動(dòng)物,獲取C5/6椎間盤(pán)組織,光鏡下觀(guān)察椎間盤(pán)形態(tài)改變,qPCR檢測(cè)椎間盤(pán)組織中TNF-αmRNA、MMP-9 mRNA表達(dá);Western blot檢測(cè)椎間盤(pán)組織中TNF-α、MMP-9蛋白表達(dá)變化。結(jié)果 PDTC給藥組(A組)椎間盤(pán)結(jié)構(gòu)破壞減輕,纖維環(huán)結(jié)構(gòu)排列有序。模型組(B組)TNF-α、MMP-9基因表達(dá)量隨實(shí)驗(yàn)時(shí)間的延長(zhǎng)而增多,早期增速較快,后期逐漸緩慢,在12周達(dá)高峰,而蛋白表達(dá)量在術(shù)后10周即達(dá)到較高水平,與12周、16周時(shí)間點(diǎn)比較無(wú)明顯差異(P0.05)。各時(shí)間點(diǎn)PDTC給藥組TNF-αmRNA、MMP-9 mRNA及蛋白表達(dá)量較模型組(B組)明顯降低(P0.01),但明顯高于假手術(shù)組(P0.01)。結(jié)論 TNF-α、MMP-9是參與大鼠頸椎間盤(pán)退變過(guò)程的重要炎性因子,TNF-α、MMP-9可能通過(guò)NF-κB信號(hào)通路參與大鼠頸椎間盤(pán)退變,PDTC有望成為頸椎間盤(pán)退變的防治藥物。
[Abstract]:Objective to observe the effect of pyrrolidine dithiocarbamate (PDTCN) on the morphology of cervical intervertebral disc and the expression of matrix metalloproteinase 9 (MMP-9) in cervical disc of rats with cervical dynamic imbalance. To investigate the inhibitory effect of PDTC on cervical disc degeneration and its mechanism, 54 SD rats were randomly divided into 3 groups. Animal model of cervical intervertebral disc degeneration. Group A: intraperitoneal injection of PDTC solution daily after modeling. Group B: intraperitoneal injection of normal saline daily after modeling. The skin of the posterior part of the neck was sutured immediately and intraperitoneally injected with saline daily. The animals were executed in batches 10 weeks and 1216 weeks after operation. C5 / 6 intervertebral disc tissue was obtained. The morphological changes of intervertebral disc were observed under light microscope. The expression of TNF- 偽 mRNA-MMP-9 mRNA in intervertebral disc tissue was detected by qPCR and the expression of TNF- 偽 -mRNA-MMP-9 protein in intervertebral disc tissue was detected by Western blot. Results the structural damage of intervertebral disc was alleviated in group A (treated with PDTC). In group B, the expression of TNF- 偽 MMP-9 gene increased with the prolongation of experimental time. The expression of TNF- 偽 MMP-9 gene increased rapidly in the early stage, slowly in the later stage, and reached its peak at 12 weeks, while the protein expression reached a higher level at 10 weeks after operation. TNF- 偽 mRNA-MMP-9 mRNA and protein expression in PDTC group were significantly lower than those in model group B (P 0.01), but significantly higher than that in sham operation group (P 0.01). Conclusion TNF- 偽 MMP-9 is involved in cervical disc degeneration in rats. TNF- 偽 -MMP-9 may be involved in the degeneration of rat cervical intervertebral disc through NF- 魏 B signaling pathway, and PDTC may be a drug to prevent and treat the degeneration of cervical intervertebral disc.
【作者單位】： 江西省人民醫(yī)院骨二科;
【基金】：江西省科技支撐計(jì)劃資助項(xiàng)目(No 20122BBG70118,20151BBG70119)
【分類(lèi)號(hào)】：R681.5

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