本文關(guān)鍵詞： 脊髓損傷 臍血干細(xì)胞 電針 運(yùn)動(dòng)誘發(fā)電位 髓鞘　出處：《濱州醫(yī)學(xué)院》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：觀察移植臍血干細(xì)胞(human umbilical cord blood stem cell, hUCBSC)聯(lián)合電針治療(Electro-acupuncture, EA)對(duì)脊髓全橫斷大鼠的神經(jīng)功能的恢復(fù)作用。方法：本次實(shí)驗(yàn)采用原代離心培養(yǎng)的方式收集培養(yǎng)臍血干細(xì)胞,調(diào)整細(xì)胞濃度為1.0×107/μL,移植備用。挑選雌性健康成年SD大鼠72只,重量220-240 g,隨機(jī)共分為4組：損傷模型組(SCI)、臍血干細(xì)胞移植組(hUCBSC)、督脈電針治療組(EA)、臍血干細(xì)胞移植聯(lián)合督脈電針治療組(hUCBSC+EA),每組18只。各組分脊髓橫斷損傷后7d、14d、28d三個(gè)監(jiān)測(cè)時(shí)間點(diǎn)。制作大鼠脊髓全橫斷模型。術(shù)后,hUCBSC籠給予一次干細(xì)胞移植,細(xì)胞濃度為1.0×107/μJ L。EA籠和EA+hUCBSC籠每天電針治療一次,電針治療的時(shí)間和頻率設(shè)置為30min、5hz,直至取材。首先用Basso-Beaie-Bresnehan scale(BBB)運(yùn)動(dòng)評(píng)分評(píng)價(jià)大鼠SCI后神經(jīng)修情況,然后運(yùn)用PowerLab數(shù)據(jù)記錄系統(tǒng)檢測(cè)不同時(shí)間點(diǎn)大鼠運(yùn)動(dòng)誘發(fā)電位的傳導(dǎo),最后行電鏡觀察,分析各組治療后脊髓修復(fù)情況在形態(tài)結(jié)構(gòu)方面的改變。結(jié)果：1、大鼠脊髓全橫斷后的,各組大鼠隨著時(shí)間的延長(zhǎng),后肢活動(dòng)均呈階梯式的恢復(fù)且聯(lián)合組與另外三組存在差異(P0.05),聯(lián)合組動(dòng)物肢體功能恢復(fù)顯著。2、電鏡觀察后發(fā)現(xiàn)時(shí)間越長(zhǎng),髓鞘的形態(tài)結(jié)構(gòu)恢復(fù)越明顯,空泡越少,腫脹越輕,且聯(lián)合治療組的新生髓鞘最多,形態(tài)規(guī)整。3、運(yùn)動(dòng)誘發(fā)電位檢測(cè)顯示,電針治療組檢測(cè)結(jié)果與臍血干細(xì)胞移植組差異不明顯(P0.05),且聯(lián)合組結(jié)果與另外三組存在差異(P0.05)。結(jié)論：脊髓損傷(SCI)后,臍血干細(xì)胞能分化遷移并作用于脊髓橫斷部位；電針刺激能夠作用于神經(jīng)損傷部位,臍血干細(xì)胞與電針刺激聯(lián)合治療能促進(jìn)神經(jīng)髓鞘再生,使大鼠脊髓損傷后的運(yùn)動(dòng)功能明顯恢復(fù)。
[Abstract]:Objective: to observe the effect of umbilical cord blood stem cell (hUCBSC) combined with electroacupuncture on the recovery of neural function in rats with spinal cord transection. Methods: umbilical cord blood stem cells were collected and cultured by primary centrifugation. The cell concentration was adjusted to 1.0 脳 107 / 渭 L. 72 adult female SD rats were selected. Weighing 220-240 g, randomly divided into 4 groups: injury model group, cord blood stem cell transplantation group, umbilical cord blood stem cell transplantation group, du vein electroacupuncture group, umbilical cord blood stem cell transplantation combined with du vein electroacupuncture treatment group, hUCBSC EAA group, 18 rats in each group. The rat spinal cord transection model was made at a time point of monitoring. After operation, the hUCBSC cage was given a stem cell transplantation. The cell concentration was 1.0 脳 10 7 / 渭 J L.EA cage and EA hUCBSC cage once a day. The time and frequency of electroacupuncture treatment was set at 30 min or 5 h, until the material was taken. The nerve repair after SCI was evaluated by Basso-Beaie-Bresnehan scale-BBB) score. Then PowerLab data recording system was used to detect the conduction of motor evoked potential in rats at different time points. Finally, electron microscopy was used to analyze the morphological and structural changes of spinal cord repair after treatment in each group. With the extension of time, the hind limb activity of each group showed a stepwise recovery, and there was a difference between the combined group and the other three groups (P 0.05). The recovery of limb function in the combined group was significant. The longer the time was, the more obvious the morphological structure of the myelin sheath was in the combined group, the longer the time was, the more obvious the recovery of the myelin structure was. The fewer vacuoles, the lighter the swelling, and the combined treatment group had the most new myelin sheath, shape. 3, motor evoked potential test showed, The results of electroacupuncture treatment group were not significantly different from those of cord blood stem cell transplantation group (P 0.05), and the results of combined group were different from those of the other three groups (P 0.05). Conclusion: after spinal cord injury (SCI), cord blood stem cells can differentiate and migrate and act on spinal cord transection. The combined treatment of umbilical cord blood stem cells and electroacupuncture stimulation can promote the regeneration of nerve myelin sheath and recover the motor function after spinal cord injury in rats.
【學(xué)位授予單位】：濱州醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R651.2

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