【摘要】：背景:艾瑞金膠原蛋白海綿理化性能穩(wěn)定,并已通過國(guó)家食品藥品監(jiān)督管理局的理化性能和生物相容性評(píng)價(jià)檢測(cè)。目的:觀察艾瑞金膠原蛋白海綿的止血效果。方法:取21只SD大鼠,建立肝臟出血?jiǎng)?chuàng)面,隨機(jī)分3組干預(yù):在實(shí)驗(yàn)組(n=7)肝臟創(chuàng)口內(nèi)部植入艾瑞金膠原蛋白海綿,同時(shí)在肝臟切口表面外敷艾瑞金膠原蛋白海綿;在陽性對(duì)照組(n=7)肝臟創(chuàng)口內(nèi)部植入醫(yī)用膠原蛋白海綿,同時(shí)在肝臟切口表面外敷醫(yī)用金膠原蛋白海綿;在空白對(duì)照組(n=7)肝臟切口表面外敷醫(yī)用紗布,記錄出血量及止血時(shí)間,干預(yù)后7,14,28 d進(jìn)行肝臟創(chuàng)面組織學(xué)觀察。結(jié)果與結(jié)論:①出血量及止血時(shí)間:3組出血量比較無差異;實(shí)驗(yàn)組、陽性對(duì)照組止血時(shí)間與空白對(duì)照組比較差異無顯著性意義,實(shí)驗(yàn)組止血時(shí)間短于陽性對(duì)照組(P≤0.05);②肝臟創(chuàng)面組織學(xué)觀察:實(shí)驗(yàn)組干預(yù)后7 d膠原材料被纖維結(jié)締組織完全包裹,炎性細(xì)胞浸潤(rùn)以中性粒細(xì)胞為主,膠原材料開始降解,周邊結(jié)締組織內(nèi)有新生毛細(xì)血管;干預(yù)后14 d,包裹膠原材料的纖維結(jié)締組織明顯增厚,中性粒細(xì)胞減少,巨噬細(xì)胞增多;干預(yù)后28 d,膠原材料完全降解,大部肝組織恢復(fù)正常,部分肝組織旁的炎性結(jié)締組織中可看到巨噬細(xì)胞、單核細(xì)胞、成纖維細(xì)胞和毛細(xì)血管。陽性對(duì)照組情況類似于實(shí)驗(yàn)組�？瞻讓�(duì)照組干預(yù)后14 d創(chuàng)傷處結(jié)締組織明顯,肝竇含有紅細(xì)胞,偶見肝組織內(nèi)出血,空泡變性;干預(yù)后28 d,創(chuàng)口處具有較厚結(jié)締組織,肝竇含有紅細(xì)胞,被復(fù)肝星狀細(xì)胞;③結(jié)果表明:艾瑞金膠原蛋白海綿對(duì)肝臟創(chuàng)傷止血效果明顯,組織相容性好。
[Abstract]:Background: the physical and chemical properties of Irikin collagen sponge are stable and have been evaluated by the State Food and Drug Administration (SFDA). Objective: to observe the hemostatic effect of irizine collagen sponge. Methods: 21 SD rats were randomly divided into 3 groups: in the experimental group (n = 7), intrahepatic collagen sponge was implanted into the liver wound, and the surface of the hepatic incision was coated with the iritin collagen sponge. Medical collagen sponge was implanted into the hepatic wound of the positive control group (nong7), and the medical gold collagen sponge was applied on the surface of the hepatic incision at the same time. Medical gauze was applied on the surface of the hepatic incision in the blank control group (nong7). The amount of bleeding and the hemostatic time were recorded. The histological observation of the liver wound was carried out on the 28th day after intervention. Results and conclusion: 1 bleeding volume and hemostatic time: there was no difference in bleeding volume among the three groups. There was no significant difference in hemostatic time between the positive control group and the blank control group. The hemostasis time in the experimental group was shorter than that in the positive control group (P 鈮，

本文編號(hào)：2385011