【摘要】：實(shí)驗(yàn)?zāi)康模簭?fù)合C3S和磷酸鈣系(磷酸氫鈣DCPA+磷酸四鈣TTCP)骨水泥，以期能改善骨水泥的降解性，提高其抗壓強(qiáng)度和力學(xué)性能，調(diào)節(jié)固化時(shí)間，提高骨水泥的生物相容性。 實(shí)驗(yàn)方法：(1)用吉爾摩(Gilmore)雙針法測(cè)定復(fù)合骨水泥的固化時(shí)間；在Instron5567型萬能材料實(shí)驗(yàn)機(jī)上測(cè)試抗壓強(qiáng)度；模擬體液(SBF)中浸泡后，骨水泥浸提液的pH值及其降解率隨時(shí)間的變化，并結(jié)合SEM和XRD微觀結(jié)構(gòu)的觀察，對(duì)骨水泥理化性質(zhì)的動(dòng)態(tài)變化規(guī)律進(jìn)行描述；(2)MTT實(shí)驗(yàn)結(jié)合實(shí)時(shí)定量研究復(fù)合骨水泥的生物相容性。 實(shí)驗(yàn)結(jié)果：(1)隨著C3S百分含量的增大，固化時(shí)間先增加后減少然后又增加。(2)隨著C3S百分含量的增加，骨水泥的抗壓強(qiáng)度先升高后降低，在C3S的含量為40%時(shí)，骨水泥的抗壓強(qiáng)度達(dá)到最大值21.6Mpa，比磷酸鈣系(TTCP+DCPA)骨水泥的強(qiáng)度稍高。(3)復(fù)合骨水泥具有較好降解性，而且隨著C3S百分含量的增加，骨水泥的降解率增大，在C3S的含量為40%時(shí)，骨水泥的降解率達(dá)到最大值10.26%，浸泡完成后復(fù)合骨水泥維持較高的抗壓強(qiáng)度，在浸泡到第7d時(shí)，抗壓強(qiáng)度達(dá)到最大值27.84MPa。復(fù)合骨水泥浸泡7d后浸泡液的pH值接近中性。(4)添加一定量的C3S骨水泥能刺激細(xì)胞的增殖，并提高CyclinD1、PCNA、SDH基因的表達(dá)。 實(shí)驗(yàn)結(jié)論：C3S的引入延長(zhǎng)了磷酸鈣系(TTCP+DCPA)骨水泥的固化時(shí)間，提高了骨水泥的降解性，在一定程度上提高了磷酸鈣系骨水泥的抗壓強(qiáng)度。制備的復(fù)合骨水泥克服了磷酸鈣系(TTCP+DCPA)骨水泥降解率低的缺點(diǎn)，，而且具有適宜的固化時(shí)間，較高的抗壓強(qiáng)度和良好的生物相容性和安全性，可作為一種潛在的骨水泥修復(fù)材料。
[Abstract]:Objective: to combine C3s with calcium phosphate (DCPA) tetracalcium phosphate (TTCP) bone cement in order to improve the degradation of bone cement, improve its compressive strength and mechanical properties, adjust the curing time, and improve the biocompatibility of bone cement. Methods: (1) the curing time of composite bone cement was determined by Gilmore (Gilmore) double needle method, and the compressive strength was measured on Instron5567 universal material experiment machine. After soaking in simulated body fluid (SBF), the pH value and degradation rate of bone cement extract changed with time. The dynamic changes of physical and chemical properties of bone cement were described by observing the microstructure of SEM and XRD. (2) the biocompatibility of composite bone cement was studied by MTT experiment and real time quantitative analysis. The results were as follows: (1) with the increase of C3s content, the curing time increased first, then decreased, and then increased. (2) with the increase of C3S content, the compressive strength of bone cement increased first and then decreased, when the content of C3S was 40%, The compressive strength of bone cement reached the maximum value of 21.6 Mpaa, which was slightly higher than that of calcium phosphate (TTCP DCPA) cement. (3) the composite bone cement had better degradability, and the degradation rate of bone cement increased with the increase of C3s content. The degradation rate of bone cement reached the maximum value of 10.26 when the content of C _ 3s was 40 and the compressive strength of composite bone cement was maintained after immersion. The compressive strength reached the maximum of 27.84 MPA at the 7th day after immersion. (4) adding a certain amount of C3s bone cement could stimulate cell proliferation and increase the expression of CyclinD1,PCNA,SDH gene. Conclusion: the introduction of C3S can prolong the curing time of calcium phosphate (TTCP DCPA) cement, improve the degradation of bone cement, and improve the compressive strength of calcium phosphate bone cement to some extent. The prepared composite bone cement overcomes the shortcoming of low degradation rate of calcium phosphate (TTCP DCPA) cement, and has the advantages of suitable curing time, high compressive strength, good biocompatibility and safety. It can be used as a potential bone cement repair material.
【學(xué)位授予單位】：河南師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.08

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