【摘要】：目的：在316L不銹鋼材料表面制備納米結構，改善316L不銹鋼表面疏水、疏油性能，觀察銀納米鍍層對血液相容性及細胞相容性的影響，并在316L不銹鋼冠脈支架表面制備銀納米鍍層，利用動物實驗探討其在生物體內抗支架內血栓及支架內再狹窄的能力，為冠狀動脈支架表面改性研究提供依據。 方法：本研究采用恒電位電沉積的方法在316L不銹鋼表面沉積銀納米鍍層，并將鋼板置于全氟硅烷溶液中浸泡，達到改變鋼板表面微觀形貌，改善鋼板疏水、疏油性能的目的。電鏡及紅外光譜檢測均證實銀納米涂層成功沉積在316L不銹鋼表面，且測試疏水角、疏油角和表面銀納米顆粒直徑。通過動態(tài)凝血實驗、抗凝血時間測定、血小板粘附實驗、溶血實驗、蛋白吸附實驗等手段，測試銀鍍膜全氟硅烷浸泡316L不銹鋼的血液相容性。并通過細胞粘附實驗、MTT法及流式細胞儀檢測細胞周期的方法測試細胞相容性。將銀鍍膜全氟硅烷浸泡316L不銹鋼具有不同疏水疏油性能的冠脈支架置入兔腹主動脈內，通過比較置入時和1個月后的造影及血管內超聲結果，并對比病理切片結果，判斷體內環(huán)境下，納米結構及疏水疏油性能改變對內皮化、血栓形成及再狹窄的影響。 結果：316L不銹鋼利用恒電位電沉積法進行表面沉積銀納米鍍層，并以全氟硅烷浸泡后，具備了一定的微相粗糙度，并改善了疏水、疏油性能，血液相容性的很多指標較裸鋼板明顯改善。比如血小板粘附數(shù)量、激活程度、白蛋白吸附量，而抗凝血性能、溶血率及纖維蛋白吸附量至少不次于裸鋼板。其中，800s和1600s電鍍處理組的抗凝血性能及抗血小板吸附激活能力好于2000s及4000s組。細胞粘附試驗，各組全氟硅烷浸泡銀納米鍍層疏水、疏油性能改善鋼板結果明顯好于裸鋼板，MTT與流式細胞儀檢測顯示，疏水、疏油性能改善對細胞相容性無明顯影響。動物實驗部分，疏水疏油性能改善的316L不銹鋼制成的支架置入動物體內后較裸支架不增加置入當時及1個月時血栓發(fā)生率，支架具有良好的組織相容性，在支架周圍組織沒有明顯的炎癥反應，各組全氟硅烷浸泡的納米銀鍍膜316L不銹鋼支架內膜厚度都較裸支架薄，內膜面積較裸支架小，其中800s，1600s，4000s均有顯著統(tǒng)計學意義（P0.05）；而中膜厚度、中膜面積，800s，1600s，，2000s較裸支架薄，具有統(tǒng)計學意義（P0.05）,4000s與裸支架類似；管腔面積方面，各組之間無統(tǒng)計學意義的差別，但是800s，1600s，2000s組較裸支架組增大。 結論：在316L不銹鋼表面進行銀鍍膜并在氟化硅烷中浸泡后，可以形成表面納米結構，可改善316L不銹鋼的疏水疏油性能，并具有良好地血液相容性及細胞相容性。銀鍍膜全氟硅烷浸泡316L不銹鋼支架不影響急性或亞急性血栓形成，并可以加速血管內皮化過程，減少支架內再狹窄的發(fā)生。
[Abstract]:Objective: to prepare nanostructures on the surface of 316L stainless steel, to improve the hydrophobic and oil-repellent properties of 316L stainless steel, and to observe the effect of silver nano-coating on blood compatibility and cytocompatibility. Silver nano-coating was prepared on the surface of 316L stainless steel coronary stent, and its ability to resist stent thrombosis and stent restenosis in vivo was investigated by animal experiments, which provided the basis for the study of surface modification of coronary stent. Methods: silver nanocrystalline coating was deposited on 316L stainless steel by potentiostatic electrodeposition, and the steel plate was immersed in perfluorosilane solution to change the surface morphology and improve the hydrophobic and oil-repellent properties of steel plate. The silver nano-coating was successfully deposited on the surface of 316L stainless steel by electron microscope and infrared spectroscopy. The hydrophobic angle, oil thinning angle and the diameter of silver nanoparticles on the surface were measured. The blood compatibility of 316L stainless steel immersed in silver coated perfluorosilane was tested by means of dynamic coagulation test, anticoagulant time measurement, platelet adhesion test, hemolysis test and protein adsorption test. Cell compatibility was tested by MTT assay and flow cytometry. Ag-coated perfluorosilane immersion 316L stainless steel coronary stents with different hydrophobic and oil-repellent properties were implanted into the abdominal aorta of rabbits. The results of angiography and intravascular ultrasound were compared at the time of implantation and 1 month later, and the results of pathological sections were compared. The effects of changes of nanostructure and hydrophobicity on endothelialization thrombosis and restenosis were evaluated in vivo. Results the silver nanocrystalline coating was deposited on the surface of W316L stainless steel by potentiostatic electrodeposition. After immersion in perfluorosilane, the microphase roughness was obtained, and the hydrophobic and oil-repellent properties were improved. Many indexes of blood compatibility were improved obviously compared with bare plate. For example, platelet adhesion, activation, albumin adsorption, anticoagulant properties, hemolysis and fibrin adsorption are at least as good as bare plates. The anticoagulant properties and the anti-platelet adsorption and activation ability were better in the electroplating groups of 800s and 1600s than those in the 200s and 400s groups. The results of cell adhesion test showed that the hydrophobicity of silver nano-coating immersed in perfluorosilane in each group was better than that of naked plate by MTT and flow cytometry. The improvement of hydrophobic and oil-repellent properties had no significant effect on the cell compatibility. In the animal experiment, the 316L stainless steel stent with improved hydrophobicity and oil repellency was put into the animal body without increasing the incidence of thrombus at the time of implantation and one month after the stent was implanted, so the stent had good histocompatibility. There was no obvious inflammatory reaction in the tissue around the scaffold. The thickness of 316L stainless steel stent immersed in perfluorosilane was thinner than that of bare stent, and the intimal area was smaller than that of bare stent, among which 800s / 1600s / 4000s was statistically significant (P0.05). However, the thickness of the middle membrane, the area of the middle membrane of 1600s / 2000s was thinner than that of the bare scaffold, which was similar to that of the bare scaffold (P0.05), but there was no significant difference in the lumen area between the groups, but it was larger in the 800s / 1600s / 2000s group than in the bare scaffold group. Conclusion: after silver plating on 316L stainless steel surface and immersion in fluorinated silane, the surface nanostructures can be formed, and the hydrophobic and oil hydrophobic properties of 316L stainless steel can be improved, with good blood compatibility and cytocompatibility. Silver coated perfluorosilane immersion 316L stainless steel stent does not affect acute or subacute thrombosis and can accelerate the process of vascular endothelialization and reduce the incidence of restenosis in the stent.
【學位授予單位】：吉林大學
【學位級別】：博士
【學位授予年份】：2013
【分類號】：R318.08

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