發(fā)布時(shí)間：2018-09-11 06:09

【摘要】：隨著科學(xué)技術(shù)的發(fā)展,各種家用電器及通信設(shè)備在人們的生活中日益普及,磁場(chǎng)在環(huán)境中的分布越來(lái)越廣泛。磁場(chǎng)的生物效應(yīng)備受人們的關(guān)注,但是磁場(chǎng)的作用機(jī)制尚無(wú)定論,大量的實(shí)驗(yàn)數(shù)據(jù)積累以及理論分析是非常有必要的。本研究選用1 mT、5 mT和10 mT的工頻磁場(chǎng)照射急性分離的小鼠皮層神經(jīng)元(15 min),應(yīng)用全細(xì)胞膜片鉗技術(shù)記錄電壓門控鈉、鉀離子通道電流,從細(xì)胞電生理學(xué)的角度研究工頻磁場(chǎng)對(duì)離子通道特性的影響,包括工頻磁場(chǎng)對(duì)電壓門控鈉、鉀通道電流的電壓、磁場(chǎng)強(qiáng)度的依賴性以及工頻磁場(chǎng)對(duì)通道穩(wěn)態(tài)激活和失活動(dòng)力學(xué)特征的影響。 本論文的研究結(jié)果包括:(1)1 mT、5 mT和10 mT工頻磁場(chǎng)影響小鼠皮層神經(jīng)元鈉通道電流,1 mT和10 mT工頻磁場(chǎng)暴露增大鈉通道電流而5 mT工頻磁場(chǎng)抑制鈉通道電流。另外,不同強(qiáng)度的工頻磁場(chǎng)影響鈉通道的激活和失活特性,但激活和失活參數(shù)的改變程度不同。(2)不同強(qiáng)度工頻磁場(chǎng)對(duì)瞬時(shí)外向鉀通道電流均有顯著的抑制作用,磁場(chǎng)強(qiáng)度不同抑制率也不同;另外,與對(duì)照組相比,不同強(qiáng)度的工頻磁場(chǎng)對(duì)瞬時(shí)外向鉀通道的激活和失活特性均有一定的影響。(3)不同強(qiáng)度的工頻磁場(chǎng)抑制延遲整流鉀通道電流,但隨著去極化電壓的增加,不同強(qiáng)度工頻磁場(chǎng)對(duì)通道電流密度的抑制率曲線的走勢(shì)不同;另外,1 mT和5 mT工頻磁場(chǎng)改變了延遲整流鉀通道的激活特性,而10 mT工頻磁場(chǎng)不改變通道的激活特性。 本課題應(yīng)用全細(xì)胞膜片鉗技術(shù),從電生理學(xué)的角度研究了不同強(qiáng)度工頻磁場(chǎng)的生物效應(yīng)。研究結(jié)果不僅為磁場(chǎng)生物效應(yīng)的研究積累了數(shù)據(jù),而且也為揭示磁場(chǎng)的作用機(jī)制奠定了數(shù)據(jù)基礎(chǔ)。
[Abstract]:With the development of science and technology, all kinds of household appliances and communication equipments are becoming more and more popular in our daily life, and the magnetic field is more and more widely distributed in the environment. The biological effect of magnetic field has attracted much attention, but the mechanism of magnetic field action has not been decided. A large amount of experimental data accumulation and theoretical analysis are very necessary. In this study, 1 mT,5 mT and 10 mT power frequency magnetic fields were used to irradiate acutely isolated mouse cortical neurons (15 min), whole-cell patch-clamp technique was used to record voltage-gated sodium and potassium channel currents. The influence of power frequency magnetic field on the characteristics of ion channel was studied from the point of view of cell electrophysiology, including the voltage of power frequency magnetic field on voltage-gated sodium and potassium channel current. The dependence of magnetic field intensity and the influence of power frequency magnetic field on the dynamic characteristics of steady state activation and inactivation of channels. The results are as follows: (1) 1 mT,5 mT and 10 mT power frequency magnetic fields affect the sodium channel currents of cortical neurons in mice (1 mT and 10 mT), and 5 mT power frequency magnetic field inhibits sodium channel currents. In addition, different intensity of power frequency magnetic field affects the activation and inactivation of sodium channel, but the change of activation and inactivation parameters is different. (2) different intensity of power frequency magnetic field has significant inhibitory effect on transient outward potassium channel current. In addition, compared with the control group, different intensity of power frequency magnetic field has certain influence on the activation and inactivation of transient outward potassium channel. (3) different intensity of power frequency magnetic field inhibits the current of delayed rectifier potassium channel. However, with the increase of depolarization voltage, the inhibition rate curves of current density of different intensity power frequency magnetic fields are different, in addition, 1 mT and 5 mT power frequency magnetic fields change the activation characteristics of delayed rectifier potassium channels. However, 10 mT power frequency magnetic field does not change the activation characteristics of the channel. In this paper, the biological effects of different intensity power frequency magnetic fields were studied by using whole-cell patch clamp technique from the electrophysiological point of view. The results not only accumulate the data for the study of the biological effect of magnetic field, but also lay the data foundation for revealing the mechanism of the magnetic field.
【學(xué)位授予單位】：天津大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.0

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉童童;王生;何麗華;葉康平;許迎春;張菲若;;工頻磁場(chǎng)慢性暴露對(duì)大鼠神經(jīng)行為的影響[J];北京大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2010年03期

2 李剛;程立君;林凌;喬曉艷;曾銳利;;30mT恒定磁場(chǎng)對(duì)神經(jīng)細(xì)胞Na~+通道特性的影響[J];光電子.激光;2009年12期

3 喬曉艷;李剛;賀秉軍;林凌;;弱激光對(duì)神經(jīng)元瞬時(shí)外向鉀通道電流特性的影響[J];光學(xué)精密工程;2007年03期

4 林凌;賈方榮;李剛;程立君;喬曉艷;;脈沖磁場(chǎng)對(duì)神經(jīng)元瞬時(shí)外向鉀電流的影響[J];光學(xué)精密工程;2008年09期

5 李怡,趙侖,邢萱,婁淑杰,何成,路長(zhǎng)林;5Hz和20Hz磁場(chǎng)對(duì)中腦神經(jīng)干細(xì)胞分化的影響[J];航天醫(yī)學(xué)與醫(yī)學(xué)工程;2002年05期

6 郭紅梅 ,郭鳳彩 ,彭燕華 ,陳秋霞;磁場(chǎng)作用對(duì)小鼠學(xué)習(xí)記憶的影響[J];生物磁學(xué);2004年03期

7 趙文春,馬偉明,趙治華,方真華,吳華;工頻磁場(chǎng)刺激小鼠MSCs體外增殖與分化的初步研究[J];生物醫(yī)學(xué)工程學(xué)雜志;2005年03期

8 黃志剛;李鋒;游洪波;陳安民;;不同強(qiáng)度工頻電磁場(chǎng)對(duì)大鼠前軟骨干細(xì)胞增殖的影響[J];實(shí)用醫(yī)學(xué)雜志;2008年20期

9 李剛;程立君;林凌;;恒定磁場(chǎng)對(duì)神經(jīng)元延遲整流鉀通道特性的影響[J];天津大學(xué)學(xué)報(bào);2009年10期

10 董良;黃玲珍;孫海英;陳彥田;沈陽(yáng);肖登明;齊瀚實(shí);;極低頻磁場(chǎng)誘導(dǎo)人肝癌細(xì)胞的凋亡(英文)[J];中國(guó)臨床康復(fù);2006年25期

相關(guān)博士學(xué)位論文 前5條

1 程立君;中等強(qiáng)度磁場(chǎng)對(duì)神經(jīng)元離子通道特性的影響及其機(jī)制探討[D];天津大學(xué);2010年

2 李飛;恒磁場(chǎng)對(duì)血管內(nèi)皮細(xì)胞生物學(xué)效應(yīng)的研究[D];第四軍醫(yī)大學(xué);2002年

3 王海燕;1Gs恒磁場(chǎng)對(duì)共培養(yǎng)內(nèi)皮細(xì)胞增殖活性和膜離子通道的影響[D];第四軍醫(yī)大學(xué);2004年

4 沈頡飛;牙科磁性附著體模擬靜磁場(chǎng)對(duì)離子通道電生理特性的影響[D];四川大學(xué);2007年

5 成波;極低頻磁場(chǎng)發(fā)生器的開發(fā)及其在神經(jīng)細(xì)胞電生理特性研究中的應(yīng)用[D];華中科技大學(xué);2007年

，

本文編號(hào)：2235824