【摘要】：為了改善聚氨酯的血液相容性能，本文主要研究了利用微波輔助表面接枝改性的方法，對聚氨酯的表面進行活化并接枝上不同分子量的聚乙二醇（PEG/MPEG/APEG）以及肝素，利用傅里葉變換衰減全反射紅外光譜法、表面水接觸角、掃描電鏡和原子力顯微鏡對聚氨酯表面特征進行分析，并通過溶血率、凝血酶原時間、補體激活等測試考察了聚氨酯表面的血液相容性能。 采用微波輔助技術將聚氨酯膜的表面進行活化。結果表明：微波輔助反應能明顯加快聚氨酯表面活化的速度，，其接枝效果受微波功率、反應時間以及反應物體積質量分數(shù)的影響。在本實驗條件下，微波功率為100W，反應物的質量分數(shù)為5%，反應時間為10分鐘時，微波輔助表面活化的效果以及效益最好。SEM表面分析表明，與傳統(tǒng)加熱反應方法相比，微波輔助反應對膜表面的破壞程度更小。 采用微波輔助技術在聚氨酯膜的表面接枝不同分子量的PEG/MPEG，并與傳統(tǒng)加熱接枝PEG/MPEG的方法進行對比。通過ATR-FTIR、接觸角分析不同方法改性后聚氨酯表面特性的變化，通過SEM、AFM觀察不同改性方法對聚氨酯表面形貌的影響，通過溶血率、血小板黏附測試、凝血時間以及補體激活測試考察不同改性方法對聚氨酯的血液相容性能的影響。結果表明：微波輔助反應能明顯加快聚氨酯表面接枝PEG/MPEG的速度，但對接枝量沒有太大的影響。SEM和AFM表面分析表明，與傳統(tǒng)加熱反應方法相比，微波輔助反應對膜表面的破壞程度更小，膜表面更加光滑平整。水接觸角的結果也表明，微波輔助反應接枝PEG/MPEG所得膜表面具有良好的親水性能。血液相容性試驗證實，利用微波輔助改性的聚氨酯表面呈現(xiàn)出更加優(yōu)異的血液相容性。 采用微波輔助技術將聚氨酯表面活化后，再將APEG接枝到聚氨酯上，最后利用EDC·HCl和NHS活化接枝上肝素。通過聚氨酯表面肝素化后性能的改變以及血液相容性能的改善來研究肝素對聚氨酯表面性能的影響。血液相容性測試結果證實肝素對材料血液相容性有明顯的改善作用。
[Abstract]:In order to improve the blood compatibility of polyurethane, the surface of polyurethane was activated and grafted with poly (ethylene glycol) (PEG/MPEG/APEG) and heparin (heparin) with different molecular weight by microwave assisted surface grafting. Fourier transform attenuated total reflectance infrared spectroscopy (FTIR), surface water contact angle, scanning electron microscope (SEM) and atomic force microscope (AFM) were used to analyze the surface characteristics of polyurethane. The blood compatibility of polyurethane surface was investigated by complement activation test. The surface of polyurethane film was activated by microwave assisted technique. The results showed that microwave-assisted reaction could accelerate the activation of polyurethane surface, and the grafting effect was influenced by microwave power, reaction time and mass fraction of reactants. Under the experimental conditions, microwave power is 100 W, mass fraction of reactant is 5, reaction time is 10 minutes, the effect and benefit of microwave assisted surface activation is the best. SEM surface analysis shows that compared with traditional heating reaction method, microwave assisted surface activation is better than conventional heating reaction method. The microwave assisted reaction has less damage to the film surface. The microwave-assisted technique was used to graft PEG- / MPEGon with different molecular weights on the surface of polyurethane membrane and compared with the conventional heating grafted PEG/MPEG. The surface properties of polyurethane modified by different methods were analyzed by ATR-FTIR.The effects of different modification methods on the surface morphology of polyurethane were observed by SEMAFM. The hemolysis rate and platelet adhesion were measured. The effects of different modification methods on the blood compatibility of polyurethane were investigated by coagulation time and complement activation test. The results show that the microwave-assisted reaction can obviously accelerate the grafting rate of PEG/MPEG on polyurethane surface, but the grafting amount has no great influence on AFM surface analysis. The microwave assisted reaction has less damage to the film surface, and the film surface is more smooth and smooth. The results of water contact angle also showed that the surface of the membrane prepared by microwave-assisted reaction grafted with PEG/MPEG had good hydrophilicity. Hemocompatibility test confirmed that the surface of polyurethane modified by microwave showed better blood compatibility. The surface of polyurethane was activated by microwave-assisted technique, then APEG was grafted onto polyurethane. Finally, EDC HCl and NHS were used to activate the grafted heparin. The effects of heparin on the surface properties of polyurethane were studied through the changes of its properties after heparinization and the improvement of its blood compatibility. The results of blood compatibility test confirmed that heparin had a significant effect on improving the blood compatibility of the materials.
【學位授予單位】：暨南大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R318.08

【參考文獻】

相關期刊論文 前10條

1 胡衛(wèi)建,葉長寧,李良忠,朱明華,曾怡,蔣麗;生物材料靜態(tài)血清接觸對總補體溶血活性(CH_(50))影響的實驗研究[J];北京生物醫(yī)學工程;2001年03期

2 熊軍;孫芳;杜洪光;;丙酮-二正丁胺滴定法測定聚氨酯中的異氰酸酯基[J];分析試驗室;2007年08期

3 胡玉才,林潔,李敏,呂菊波,劉淑芬;微波輻射對有機合成反應的促進作用[J];輻射研究與輻射工藝學報;2005年03期

4 謝興益,鐘銀屏,劉日方,何成生,樊翠蓉,樂以倫;生物穩(wěn)定聚氨酯材料研究進展[J];高分子材料科學與工程;2000年04期

5 甘慧,楊軍,孫萍,王全立;人類補體C3研究進展[J];國外醫(yī)學.臨床生物化學與檢驗學分冊;2004年06期

6 王慶霞,陳虹;微波在液相有機合成中的應用[J];能源技術與管理;2004年05期

7 王園園;宋華;孫興龍;柳艷修;宋春紅;;微波輻射技術在有機合成中的應用[J];精細石油化工進展;2008年08期

8 張承焱;漫談醫(yī)用聚氨酯[J];世界橡膠工業(yè);2003年05期

9 楊立峰;許建霞;奚廷斐;;生物材料血液相容性的研究與評價[J];生物醫(yī)學工程學雜志;2009年05期

10 朱明華，吳增樹，曾怡，黃聘和;生物材料靜態(tài)血清接觸對免疫球蛋白及C_3活化的實驗研究[J];透析與人工器官;1996年01期

本文編號：2169607