【摘要】：毛囊來源的神經嵴干細胞(Epidermal Neural Crest Stem Cell,EPI-NCSC)由于取材方便,具有多種分化潛能,是一種具有良好應用前景的組織工程種子細胞。目前,在神經損傷修復領域中,EPI-NCSC主要被應用于脊髓損傷的修復。為了探討EPI-NCSC對周圍神經缺損的修復作用,對原代培養(yǎng)的GFP-SD大鼠來源的EPI-NCSC的體外性質進行了考察,并以其為種子細胞,將其等量與細胞外基質(Extracellular matrix,ECM)混合后,預置入聚乳酸-聚羥基乙酸共聚物(Poly lactic acid co glycolic acid copolymer,PLGA)導管中,同時,以等量的達爾伯克(氏)改良伊格爾(氏)培養(yǎng)基(Dulbecco's Modified Eagle's medium,DMEM)代替EPI-NCSC作為對照,以用于修復大鼠坐骨神經10 mm距離的缺失。噻唑藍(Methyl thiazolyl tetrazolium,MTT)比色分析結果顯示,EPI-NCSC在PLGA膜上的初期粘附率為89.7%。在第1、3、5、7天細胞相對增殖率分別為89.3%、87.6%、85.6%和96.6%。細胞周期與DNA倍體分析表明,與PLGA共培養(yǎng)的EPI-NCSC與單獨培養(yǎng)的EPI-NCSC相比較,二者的細胞周期變化趨勢相同,增殖指數(shù)變化趨勢也相同。在神經導管移植4周,術部實現(xiàn)了組織學水平的修復。大鼠手術一側后肢感覺功能有所恢復,坐骨神經指數(shù)有所提高。研究結果表明,在PLGA導管中預置EPI-NCSC,有望實現(xiàn)較好的周圍神經缺損的修復效果。
[Abstract]:The neural cristal stem cell (Epidermal Neural Crest Stem Cell-EPI-NCSC) derived from hair follicle is a kind of tissue engineering seed cell with good application prospect because of its convenient material collection and multiple differentiation potential. At present, EPI-NCSC is mainly used in the repair of spinal cord injury in the field of nerve injury repair. In order to investigate the effect of EPI-NCSC on repairing peripheral nerve defects, the in vitro properties of EPI-NCSC derived from primary cultured GFP-SD rats were investigated. The same amount of EPI-NCSC was mixed with the extracellular matrix (Extracellular) as seed cells. A polylactic acid-poly-glycolic acid copolymer (Poly lactic acid co glycolic acid copolymerium PLGA) catheter was prepositioned. At the same time, EPI-NCSC was replaced by a modified (Dulbecco's Modified Eagle's medium (DMEM) of the same amount as Darbek's medium. To repair the loss of 10 mm distance of sciatic nerve in rats. The results of (Methyl thiazolyl tetrazolium MTT colorimetric analysis showed that the initial adhesion rate of EPI-NCSC on PLGA membrane was 89.7%. On the 7th day, the relative proliferation rate of the cells was 87.6% and 96.6%, respectively. Cell cycle and DNA ploidy analysis showed that compared with EPI-NCSC co-cultured with PLGA and EPI-NCSC cultured alone, the cell cycle change trend was the same and proliferation index change trend was the same. After 4 weeks of nerve conduit transplantation, the histological level was restored in the surgical department. The sensory function and sciatic nerve index of one hind limb of the rats were recovered and the sciatic nerve index was improved. The results show that EPI-NCSC-preimplantation in PLGA catheter is expected to achieve a better repair effect of peripheral nerve defect.
【作者單位】： 西南大學生命科學學院;淡水魚類資源與生殖發(fā)育教育部重點實驗室;第三軍醫(yī)大學大坪醫(yī)院野戰(zhàn)外科研究所創(chuàng)傷、燒傷與復合傷國家重點實驗室;重慶醫(yī)科大學生理學教研室神經科學研究中心;
【基金】：國家重點基礎研究發(fā)展計劃(973計劃)(No.2012CB518106) 創(chuàng)傷、燒傷與復合傷國家重點實驗室Ⅰ類研究課題(No.SKLZZ201003) 國家自然科學基金(Nos.31271036,81371341)資助~~
【分類號】：R318;Q813

【共引文獻】

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